02695nas a2200265 4500008004100000022001400041245011400055210006900169260007400238300001400312490000700326520177900333653002302112653001202135653001402147653000902161653001002170100002202180700002302202700002102225700001802246700001702264700002502281856012302306 2012 eng d a1380-374300aMicrosatellite mapping identifies TTKST-effective stem rust resistance gene in wheat cultivars VL404 and Janz0 aMicrosatellite mapping identifies TTKSTeffective stem rust resis aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcDEC a1757-17650 v303 a

Wheat cultivar VL404 carries seedling resistance to Puccinia graminis f. sp. tritici pathotype TTKST. Monogenic segregation for seedling resistance was observed in a VL404/WL711 recombinant inbred line population and the resistance locus was temporarily designated SrVL. Bulked segregant analysis using Diversity Arrays Technology markers located SrVL on chromosome 2BL. Detailed simple sequence repeat mapping placed SrVL between gwm120 and wmc175, both at genetic distances of 3.3 cM. Based on adult plant responses of Janz and VL404 in India and Kenya, we expected these cultivars to carry the same gene against TTKST. A subset of Diamondbird/Janz doubled haploid (DH) population showed monogenic segregation, when tested against TTKST and the locus was temporarily named SrJNZ. SrVL-linked markers gwm120 and wmc175 flanked SrJNZ at a similar genetic distance, thereby confirming our hypothesis. Chromosome 2BL carries Sr9, Sr16 and Sr28. Sr9 is a multi-allelic locus and all known alleles of Sr9 and Sr16 are ineffective against TTKSK and its derivatives. A recombination value of 16.7 cM between Sr9g-linked stripe rust resistance gene Yr7 and SrJNZ in Diamondbird/Janz DH population suggested that SrJNZ is not an allele at the Sr9 locus. Based on comparison of published genetic distances between Lr13, Sr9, Sr28 and Sr16 with that observed in this study, we concluded SrVL and SrJNZ to be Sr28. This gene was contributed by a common parent Gabo, which also exhibited resistance against TTKST. Sr28-linked markers gwm120 and wmc175 confirmed the presence of this gene in a high proportion of Australian cultivars that showed stem rust resistance in Kenya. These markers can be used for marker-assisted pyramiding of Sr28 with other stem rust resistance genes.

10aDisease resistance10aMarkers10aStem rust10aUg9910aWheat1 aBansal, Urmil, K.1 aZwart, Rebecca, S.1 aBhavani, Sridhar1 aWanyera, Ruth1 aGupta, Vidya1 aBariana, Harbans, S. uhttps://library.ncl.res.in/content/microsatellite-mapping-identifies-ttkst-effective-stem-rust-resistance-gene-wheat-002839nas a2200325 4500008004100000245014800041210006900189260000800258490000700266520174000273653001002013653002202023653001902045653002602064653001402090653002202104653002202126653002702148653001202175653001402187653000802201653001002209653004502219653002702264100002802291700001902319700001902338700002602357856013002383 2017 eng d00aGenetic variability in Indian populations of banana corm weevil [Cosmopolites sordidus (Coleoptera: Curculionidae)] assessed by RAPDs and AFLPs0 aGenetic variability in Indian populations of banana corm weevil  cSEP0 v373 a

Cosmopolites sordidus (Germar), commonly known as banana corm weevil, is an important economic pest in Asia that can cause severe yield loss depending upon the stage at which infestation occurs. In spite of its economic importance, little is known about the population structure of this pest in India. Random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) were used to characterize the population genetic structure of C. sordidus collected from five hot spot locations in India. Nineteen RAPD primers and five selective AFLP primer combinations generated 147 and 304 amplification products, respectively. UPGMA dendrograms generated with both marker systems failed to reveal populations clustered based on geographic distance, which was confirmed by the Mantel test, which did not show a strong correlation between genetic distance and geographic distance. Values of indices of genetic identity showed that the populations were closely related. Though the gene flow estimate (Nm) between the populations was 0.469, suggesting restricted gene flow, the populations are not genetically distinct. These observations suggest that the range expansion of this banana pest in India has taken place through transport of infested corms and plant material, resulting in genetically close populations that are geographically distinct. These results provide important information on the population structure of this pest in India, which will aid in designing suitable strategies for its control and management, especially with respect to insecticide resistance.

10aAflps10aArbitrary Primers10aBemisia-tabaci10aCosmopolites Sordidus10adiversity10aGenetic diversity10aGermar Coleoptera10ainsecticide resistance10aMarkers10aMusa Spp.10aPcr10aRapds10aRestricted Gene Flow Natural-populations10aScirpophaga-incertulas1 aYadav, Sweta, Kumari U.1 aSingh, Jyotsna1 aPadmanaban, B.1 aKumar, Lalitha, Sunil uhttps://library.ncl.res.in/content/genetic-variability-indian-populations-banana-corm-weevil-cosmopolites-sordidus-coleoptera