01557nas a2200217 4500008004100000022001400041245012600055210006900181260011700250300001000367490000600377520065400383653002701037653001801064653001701082653003701099653002001136100002301156700003301179856012701212 2010 eng d a0973-626300aStudies of antimicrobial activity of turmeric (Curcuma Longa L.) leaves and rhizomes collected during plant growth phases0 aStudies of antimicrobial activity of turmeric Curcuma Longa L le aSECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIAbRESEARCH JOURNAL BIOTECHNOLOGYcMAY a24-280 v53 a

Turmeric is the processed underground rhizome used as spice, herbal medicines, dying agent and cosmetics. The leaves and rhizomes of this plant were collected at periodic intervals. These samples were air dried, powdered and extracted with MeOH and EtOH. Antibacterial activity of these extracts was determined against three different index microbes, gram-positive cocci, gram-negative rod and cocci using well diffusion method with appropriate controls. Antimicrobial activity of the 4-month-old leaves was found to be the maximum while that of mature and fresh rhizomes was the maximum against both gram-positive and Gram-negative bacteria.

10aantibacterial activity10aCurcuma longa10aLeaf extract10aMinimum inhibitory concentration10arhizome extract1 aShirgurkar, M., V.1 aThengane, Shubhada, Ratnakar uhttps://library.ncl.res.in/content/studies-antimicrobial-activity-turmeric-curcuma-longa-l-leaves-and-rhizomes-collected-002105nas a2200253 4500008004100000022001400041245016500055210006900220260009900289300001400388490000800402520111200410653000901522653001801531653002901549653001301578653002001591100002101611700002401632700002101656700002401677700002201701856012801723 2012 eng d a0308-814600aDiscovering bisdemethoxycurcumin from curcuma longa rhizome as a potent small molecule inhibitor of human pancreatic alpha-amylase, a target for type-2 diabetes0 aDiscovering bisdemethoxycurcumin from curcuma longa rhizome as a aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcDEC a2638-26420 v1353 a

Curcuma longa rhizome is used extensively in culinary preparations in Far East and South-East Asia. Health benefits of curcuminoids from C. longa as antioxidants, anti-cancer and anti-inflammatory molecules have been well documented. We report here for the first time that Bisdemethoxycurcumin (BDMC) from C. longa, acts as an inhibitor to inactivate human pancreatic alpha-amylase, a therapeutic target for oral hypoglycemic agents in type-2 diabetes. Bioactivity guided isolation of rhizome isopropanol extract led to the identification by HPLC and NMR of BDMC as a lead small molecule inhibitor of porcine and human pancreatic alpha-amylase with an IC50 value of 0.026 and 0.025 mM, respectively. Kinetic analysis revealed that using starch as the substrate, HPA exhibited an uncompetitive mode of inhibition with an apparent K-i of 3.0 mu M. The study gains importance as BDMC could be a good drug candidate in development of new inhibitors of HPA and of functional foods for controlling starch digestion in order to reduce post-prandial hyperglycemia. (C) 2012 Elsevier Ltd. All rights reserved.

10aBDMC10aCurcuma longa10aHuman pancreatic amylase10akinetics10aType-2 diabetes1 aPonnusamy, Sudha1 aZinjarde, Smita, S.1 aBhargava, Shobha1 aRajamohanan, P., R.1 aRaviKumar, Ameeta uhttps://library.ncl.res.in/content/discovering-bisdemethoxycurcumin-curcuma-longa-rhizome-potent-small-molecule-inhibitor-003123nas a2200265 4500008004100000022001400041245017300055210006900228260000800297300001100305490000700316520221700323653001202540653001102552653002302563653002302586653000902609653001802618653002402636653001402660100002002674700002302694700002202717856011802739 2021 eng d a0145-888400aImprovement in serum amylase and glucose levels in diabetic rats on oral administration of bisdemethoxycurcumin from Curcuma longa and limonoids from Azadirachta indica0 aImprovement in serum amylase and glucose levels in diabetic rats cAPR ae136740 v453 a

Curcuma longa and Azadirachta indica are traditionally used in Indian cuisine and Ayurvedic medicine as nutraceuticals against diabetes. The crude C. longa isopropanol extract, bisdemethoxycurcumin (BDMC), the purified bioactive component from C. longa, and limonoids azadiradione, gedunin from A. indica, are able to inhibit in vitro the antidiabetic target human pancreatic alpha-amylase independently. However, no reports on their in vivo efficacy in animal models exist. Thus, the antidiabetic effect of these orally administered human pancreatic alpha-amylase inhibitors was performed on streptozotocin-induced Sprague-Dawley rats. Initially, the normal rats were treated with test compounds (10-100 mg/kg of body weight) in corn oil (5 ml/kg), and as no lethality was observed in these doses, further studies were carried out with lowest concentration of 10 mg/kg of body weight. A reduction in area under curve (AUC) suggested glucose-lowering effect of these compounds in starch fed diabetic rats. The efficacy study showed a significant improvement in body weight, blood glucose levels, serum amylase, and fructosamine levels as well in other serum parameters associated with diabetes with respect to liver and renal functions. Hence, under in vivo conditions, inhibition of alpha-amylase activity by BDMC and limonoids affirms it as one of the mechanisms of action resulting in reduction of blood glucose levels. Practical applications Bisdemethoxycurcumin from C. longa and limonoids, namely, azadiradione and gedunin, from A. indica are potent inhibitors of the antidiabetic target human pancreatic alpha-amylase. Oral Starch Tolerance Test (OSTT) and 28-day efficacy study to check the effect of these orally administered inhibitors in diabetic rat models showed significant improvements in serum blood glucose and amylase levels as well as in other diabetes related serum parameters, namely, bilirubin, lipids, lactate dehydrogenase, alkaline phosphatase, and urea. The study contributes to understanding the action and efficacy of these pancreatic alpha-amylase inhibitors and suggests a potential role for them as nutraceuticals/therapeutics in management of post-prandial hyperglycemia.

10a&\#820810a&\#94510aAmylase inhibitors10aAzadiractha indica10aBDMC10aCurcuma longa10ain vivo rat studies10aLimonoids1 aRamkumar, Sudha1 aThulasiram, V., H.1 aRaviKumar, Ameeta uhttps://library.ncl.res.in/content/improvement-serum-amylase-and-glucose-levels-diabetic-rats-oral-administration