02563nas a2200241   4500008004100000022001400041245012300055210006900178260009500247300001200342490000800354520164900362653001802011653001402029653002102043653001602064653002802080653001402108100002202122700002502144700002402169856012802193       2012                        eng d  a0006-291X00aTrehalose as an indicator of desiccation stress in Drosophila melanogaster larvae: a potential marker of anhydrobiosis0 aTrehalose as an indicator of desiccation stress in Drosophila me  a525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USAbACADEMIC PRESS INC ELSEVIER SCIENCEcMAR  a638-6420 v4193 a<p>In the current scenario of global climate change, desiccation is considered as one of the major environmental stressors for the biota exposed to altered levels of ambient temperature and humidity. Drosophila melanogaster, a cosmopolitan terrestrial insect has been chosen as a humidity-sensitive bioindicator model for the present study since its habitat undergoes frequent stochastic and/or seasonally aggravated dehydration regimes. We report here for the first time the occurrence of anhydrobiosis in D. melanogaster larvae by subjecting them to desiccation stress under laboratory conditions. Larvae desiccated for ten hours at &lt;5% relative humidity could enter anhydrobiosis and could revive upon rehydration followed by resumption of active metabolism. As revealed by FIR and HPLC analyzes, our findings strongly indicated the synthesis and accumulation of trehalose in the desiccating larvae. Biochemical measurements pointed out the desiccation-responsive trehalose metabolic pathway that was found to be coordinated in concert with the enzymes trehalose 6-phosphate synthase and trehalase. Further, an inhibitor-based experimental approach using deoxynojirimycin, a specific trehalase inhibitor, demonstrated the pivotal role of trehalose in larval anhydrobiosis of D. melanogaster. We therefore propose trehalose as a potential marker for the assessment of anhydrobiosis in Drosophila. The present findings thus add to the growing list of novel biochemical markers in specific bioindicator organisms for fulfilling the urgent need of environmental biomonitoring of climate change. (C) 2012 Elsevier Inc. All rights reserved.</p>10aAnhydrobiosis10abiomarker10aDeoxynojirimycin10aDesiccation10aDrosophila melanogaster10atrehalose1 aThorat, Leena, J.1 aGaikwad, Sushama, M.1 aNath, Bimalendu, B.  uhttps://library.ncl.res.in/content/trehalose-indicator-desiccation-stress-drosophila-melanogaster-larvae-potential-marker-201879nas a2200253   4500008004100000022001400041245011900055210006900174260006300243300001200306490000600318520096300324653001401287653001001301653001501311100002501326700002301351700002401374700002101398700002201419700002701441700002501468856013201493       2013                        eng d  a1791-299700aProteomic study reveals downregulation of apolipoprotein A1 in plasma of poorly controlled diabetes: a pilot study0 aProteomic study reveals downregulation of apolipoprotein A1 in p  aPOB 18179, ATHENS, 116 10, GREECEbSPANDIDOS PUBL LTDcFEB  a495-4980 v73 a<p>Proteomic approaches aid in gaining a better understanding of the pathophysiology of diabetic complications. In view of this, differential protein expression in diabetic plasma samples was studied by a combination of proteomic and western blot analyses. Diabetic plasma samples were categorized based on glycated haemoglobin levels as controlled diabetes (CD; 7-8%), poorly controlled diabetes (PCD; &gt;8%) and non-diabetic control (ND;&lt;6.4%). Two-dimensional electrophoresis and liquid chromatography-mass spectrometry revealed differential expression of proteins including upregulation of fibrinogen and haptoglobin and downregulation of vitamin D binding protein, alpha-1-antitrypsin, transthyretin and apolipoprotein A1 (Apo A1) in diabetic compared with non-diabetic plasma samples. Amongst these proteins, Apo A1 downregulation was prominent in PCD. Downregulation of Apo A1 may serve as an early predictive marker of diabetic complications.</p>10abiomarker10aHbA1c10aproteomics1 aBhonsle, Hemangi, S.1 aKorwar, Arvind, M.1 aChougale, Ashok, D.1 aKote, Sachin, S.1 aDhande, Nitin, L.1 aShelgikar, Kishore, M.1 aKulkarni, Mahesh, J.  uhttps://library.ncl.res.in/content/proteomic-study-reveals-downregulation-apolipoprotein-a1-plasma-poorly-controlled-diabetes-001253nas a2200361   4500008004100000022001400041245014600055210006900201260000800270300001400278490000700292653002300299653001800322653001400340653001500354653003000369100001800399700004000417700002000457700001700477700002600494700002300520700002200543700002100565700002400586700002600610700003200636700002300668700001900691700003100710700002700741856012300768       2024                        eng d  a0939-475300aCirculatory levels of lysophosphatidylcholine species in obese adolescents: Findings from cross-sectional and prospective lipidomics analyses0 aCirculatory levels of lysophosphatidylcholine species in obese a  cJUL  a1807-18160 v3410aAdolescent obesity10aAsian Indians10abiomarker10aLipidomics10aLysophosphatidyl- choline1 aSharma, Sapna1 aSubrahmanyam, Yalamanchili, Venkata1 aRanjani, Harish1 aSidra, Sidra1 aParmar, Dharmeshkumar1 aVadivel, Sangeetha1 aKannan, Shanthini1 aGrallert, Harald1 aUsharani, Dandamudi1 aAnjana, Ranjit, Mohan1 aBalasubramanyam, Muthuswamy1 aMohan, Viswanathan1 aJerzy, Adamski1 aPanchagnula, Venkateswarlu1 aGokulakrishnan, Kuppan  uhttps://library.ncl.res.in/content/circulatory-levels-lysophosphatidylcholine-species-obese-adolescents-findings-cross