01996nas a2200241   4500008004100000022001400041245019800055210006900253260009500322300001200417490000800429520105500437653001401492653000801506653000901514653002301523653002401546653001001570653001301580100002101593700001101614856012901625       2005                        eng d  a0006-291X00aPurification and properties of a low molecular weight 1,4-beta-D-glucan glucohydrolase having one active site for carboxymethyl cellulose and xylan from an alkalothermophilic Thermomonospora sp0 aPurification and properties of a low molecular weight 14betaDglu  a525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USAbACADEMIC PRESS INC ELSEVIER SCIENCEcAPR  a111-1160 v3293 a<p>A low molecular weight 1,4-beta-D-glucan glucohydrolase from an extracellular culture filtrate of Thermomonospora sp. was purified to homogeneity. The molecular weight of the purified enzyme was 14.2 kDa by MALDI-TOF analysis and is in agreement with SDS-PAGE and gel filtration chromatography. The purified enzyme exhibited both endocarboxymethyl cellulase and endoxylanase activities. A kinetic method was employed to study the active site of the enzyme that hydrolyzes both carboxymethyl cellulose and xylan. The experimental data coincide well with the theoretical values calculated for the case of a single active site. Conformation and microenvironment at the active site was probed with fluorescent chemo-affinity labeling using o-phthalaidehyde as the chemical initiator. Formation of isoindole derivative resulted in complete inactivation of the enzyme to hydrolyze both xylan and CMC as judged by fluorescence studies corroborating a single active site for the hydrolysis of xylan and CMC. (C) 2005 Elsevier Inc. All rights reserved.</p>10aCellulase10aCMC10aOPTA10asingle active site10aThermomonospora sp.10axylan10aXylanase1 aJagtap, Sharmili1 aRao, M  uhttps://library.ncl.res.in/content/purification-and-properties-low-molecular-weight-14-beta-d-glucan-glucohydrolase-having-002528nas a2200241   4500008004100000022001400041245015000055210006900205260008000274300001200354490000700366520158700373653001901960653002201979653000802001653002602009653001802035653002002053100002902073700002302102700003102125856013002156       2013                        eng d  a1097-395800aMicrobial oxidation of medium chain fatty alcohol in the synthesis of sophorolipids by candida bombicola and its physicochemical characterization0 aMicrobial oxidation of medium chain fatty alcohol in the synthes  aTIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANYbSPRINGER HEIDELBERGcMAR  a173-1810 v163 a<p>Sophorolipids (SL) are glycolipid biosurfactants abundantly produced from different feedstocks by yeasts and have been widely developed for various applications. The amphipathic structures of sophorolipids imparts to them surfactant type properties. These biosurfactants are readily isolated in high yield and are nontoxic and biodegradable, which make them industrially interesting as surfactants or emulsifiers. Sophorolipid production was carried out using glucose as a hydrophilic source and lauryl alcohol C12-14, as a hydrophobic source by Candida bombicola (ATCC 22214). Process economics and environmental attractiveness was increased by using a low cost substrate. Optimization studies were carried out where the effect of glucose concentration, pH, temperature and metal ions on sophorolipid production was studied. Optimum production of sophorolipid obtained using lauryl alcohol (SLLA) was achieved after 96 h, the initial pH of the medium was 6.0 with 10 % glucose at 30 A degrees C. Primary characterization of SLLA was done by FTIR. The SLLA showed high physicochemical properties in terms of the surface activities when compared with synthetic surfactants including dodecyl tetraethylene glycol ether and sodium lauryl sulfate. The surface tension of SLLA thus obtained was found to be 24 mN/m with a critical micellar concentration (CMC) value of 0.68 mg/L. Antimicrobial testing showed that SLLA have potent antimicrobial activity against both gram negative bacteria, Escherichia coli (ATCC 8739) and gram positive cocci, Staphylococcus aureuss (ATCC 2079).</p>10aBiosurfactants10aCandida bombicola10aCMC10aLauryl alcohol C12-1410asophorolipids10aSurface tension1 aPulate, Vrushali, Dengle1 aBhagwat, Sunil, S.1 aPrabhune, Asmita, Ashutosh  uhttps://library.ncl.res.in/content/microbial-oxidation-medium-chain-fatty-alcohol-synthesis-sophorolipids-candida-bombicola-0