02935nas a2200253 4500008004100000022001400041245022400055210006900279260007300348300001000421490000800431520192100439653001702360653001002377653001402387653002302401653002102424653002402445653001002469100002102479700002202500700002802522856013102550 2008 eng d a1570-023200aDevelopment and application of a method for analysis of lufenuron in wheat flour by gas chromatography-mass spectrometry and confirmation of bio-efficacy against Tribolium castaneum (Herbst) (Coleoptera : Tenebrionidae)0 aDevelopment and application of a method for analysis of lufenuro aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJAN a16-210 v8613 a

A new analytical method using gas chromatography with mass spectrometry (GC-MS) for the quantitative determination of lufenuron, a benzoylphenylurea (BPU) class of insecticide, from wheat flour has been developed and applied for time-dependant residue monitoring in treated wheat flour. The analyte was extracted from wheat flour by a single step solid-liquid extraction by using ethyl acetate and subsequently cleaned up using the Primary Secondary Amine as a sorbent prior to GC-MS analysis. The present method provides sufficient sensitivity as reflected by the values of limit of detection (LOD) and limit of quantification (LOQ), 5 ng/mL (S/N similar to 3) and 50 ng/mL (the lowest validation point on the calibration curve), respectively. The calibration curve showed an excellent linearity in the concentration range of 50-1000 ng/mL (r(2) = 0.998). The average recovery for spiked samples at three concentrations (150, 300, and 450 ng/g) was 98.23 +/- 2.52% R.S.D. The method was applied for the determination of lufenuron residues in treated wheat flour samples. Simultaneous determination of bio-efficacy of lufenuron residues was also carried out against the red flour beetle, Tribolium castaneum to correlate the actual residual effect of lufenuron as detected by the analytical method, over a period of 3 months. The findings revealed that the residual concentration of lufenuron were neither uniform nor in descending order over a period of 3 months in wheat flour, possibly because of an uneven dispersal in the treated wheat which was subsequently milled into flour, as confirmed by GC-MS analysis. However, the residues of lufenuron were sufficient to produce 100% mortality of T. castaneum larvae up to 3 months. The results have been discussed in view of the potential of lufenuron as a candidate molecule for the control of stored product pests. (c) 2007 Elsevier B.V. All rights reserved.

10abio-efficacy10aGC-MS10alufenuron10amethod development10aresidue analysis10aTribolium castaneum10aWheat1 aAhire, Kedar, C.1 aArora, Manjit, S.1 aMukherjee, Samindra, N. uhttps://library.ncl.res.in/content/development-and-application-method-analysis-lufenuron-wheat-flour-gas-chromatography-mass-002695nas a2200265 4500008004100000022001400041245011400055210006900169260007400238300001400312490000700326520177900333653002302112653001202135653001402147653000902161653001002170100002202180700002302202700002102225700001802246700001702264700002502281856012302306 2012 eng d a1380-374300aMicrosatellite mapping identifies TTKST-effective stem rust resistance gene in wheat cultivars VL404 and Janz0 aMicrosatellite mapping identifies TTKSTeffective stem rust resis aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcDEC a1757-17650 v303 a

Wheat cultivar VL404 carries seedling resistance to Puccinia graminis f. sp. tritici pathotype TTKST. Monogenic segregation for seedling resistance was observed in a VL404/WL711 recombinant inbred line population and the resistance locus was temporarily designated SrVL. Bulked segregant analysis using Diversity Arrays Technology markers located SrVL on chromosome 2BL. Detailed simple sequence repeat mapping placed SrVL between gwm120 and wmc175, both at genetic distances of 3.3 cM. Based on adult plant responses of Janz and VL404 in India and Kenya, we expected these cultivars to carry the same gene against TTKST. A subset of Diamondbird/Janz doubled haploid (DH) population showed monogenic segregation, when tested against TTKST and the locus was temporarily named SrJNZ. SrVL-linked markers gwm120 and wmc175 flanked SrJNZ at a similar genetic distance, thereby confirming our hypothesis. Chromosome 2BL carries Sr9, Sr16 and Sr28. Sr9 is a multi-allelic locus and all known alleles of Sr9 and Sr16 are ineffective against TTKSK and its derivatives. A recombination value of 16.7 cM between Sr9g-linked stripe rust resistance gene Yr7 and SrJNZ in Diamondbird/Janz DH population suggested that SrJNZ is not an allele at the Sr9 locus. Based on comparison of published genetic distances between Lr13, Sr9, Sr28 and Sr16 with that observed in this study, we concluded SrVL and SrJNZ to be Sr28. This gene was contributed by a common parent Gabo, which also exhibited resistance against TTKST. Sr28-linked markers gwm120 and wmc175 confirmed the presence of this gene in a high proportion of Australian cultivars that showed stem rust resistance in Kenya. These markers can be used for marker-assisted pyramiding of Sr28 with other stem rust resistance genes.

10aDisease resistance10aMarkers10aStem rust10aUg9910aWheat1 aBansal, Urmil, K.1 aZwart, Rebecca, S.1 aBhavani, Sridhar1 aWanyera, Ruth1 aGupta, Vidya1 aBariana, Harbans, S. uhttps://library.ncl.res.in/content/microsatellite-mapping-identifies-ttkst-effective-stem-rust-resistance-gene-wheat-002233nas a2200313 4500008004100000022001400041245007500055210006900130260007400199300001200273490000700285520122400292653002301516653001401539653003001553653002701583653001001610100002601620700002001646700001601666700001701682700001501699700002101714700002001735700002201755700001801777700001801795856010601813 2013 eng d a1380-374300aMolecular mapping of leaf rust resistance gene Lr15 in hexaploid wheat0 aMolecular mapping of leaf rust resistance gene Lr15 in hexaploid aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcMAR a743-7470 v313 a

Leaf rust is a widespread and commonly occurring rust disease of wheat. Genetic resistance is the most economical method of reducing losses due to leaf rust. Lr15 has been shown to be present on wheat chromosome 2D and is reported to be a seedling resistance gene. However, tightly linked markers associated with Lr15 have not been reported to date. To identify molecular markers linked to Lr15, an F-2 mapping population of Thatcher x Thatcher-Lr15 was generated. Available wheat simple sequence repeat markers were utilized in parental screening and polymorphic markers were used to analyze the entire population of 221 plants. Phenotypic evaluations of the F-2-derived F-3 progenies with Puccinia triticina Eriks. pathotype 162A (93R15) confirmed the monogenic inheritance of Lr15. The linkage group representing chromosome 2DS was constructed at LOD 4.0 which revealed the closest flanking markers Xgwm4562 and Xgwm102 at a distance of 3.1 and 9.3 cM, respectively. Furthermore, utilization of these flanking markers in combination has successfully identified wheat lines with or without Lr15. These markers could potentially be useful in gene pyramiding with other genes to enhance rust resistance in wheat.

10aDisease resistance10aLeaf rust10aMarker-assisted selection10aSimple sequence repeat10aWheat1 aDholakia, Bhushan, B.1 aRajwade, A., V.1 aHosmani, P.1 aKhan, R., R.1 aChavan, S.1 aReddy, D., M. R.1 aLagu, Meena, D.1 aBansal, Urmil, K.1 aSaini, R., G.1 aGupta, V., S. uhttps://library.ncl.res.in/content/molecular-mapping-leaf-rust-resistance-gene-lr15-hexaploid-wheat-002060nas a2200253 4500008004100000022001400041245016900055210006900224260000800293300001400301490000700315520121400322653001601536653001201552653001201564653001701576653001001593100001401603700001401617700001401631700001301645700001701658856013101675 2022 eng d a0266-825400aScreening of osmotic stress-tolerant bacteria for plant growth promotion in wheat (Triticum aestivum L.) and brinjal (Solanum melongena L.) under drought conditions0 aScreening of osmotic stresstolerant bacteria for plant growth pr cNOV a1286-12920 v753 a

Drought stress adversely affects plant growth and productivity. Therefore, the application of plant growth-promoting bacteria is a viable option for combating drought resistance in crops. In this study, 144 bacteria were isolated from the Kutch desert soil in Gujarat. Based on osmotic stress tolerance and PGP properties, two strains, Bacillus tequilensis (KS5B) and Pseudomonas stutzeri (KS5C) were tested for their effect on wheat (Triticum aestivum L.) and brinjal (Solanum melongena L.) under drought stress conditions. Inoculation with osmotic stress-tolerant bacteria showed 15 center dot 15-29 center dot 27% enhancement in root length of wheat and 15 center dot 27-32 center dot 59% in brinjal plants. Similarly, the enhancement of shoot length ranged from 14 center dot 72 to 37 center dot 70% for wheat and 59 center dot 39-95 center dot 94% for brinjal plants. Furthermore, the inoculated plants showed significant improvement in chlorophyll content and antioxidant properties such as proline, peroxidase and polyphenol oxidase activity compared to the control. Therefore, the bacterial strains identified in this study can be used to mitigate drought stress and enhance plant biomass.

10aAntioxidant10abrinjal10adrought10aplant growth10aWheat1 aPatel, P.1 aPatil, T.1 aMaiti, S.1 aPaul, D.1 aAmaresan, N. uhttps://library.ncl.res.in/content/screening-osmotic-stress-tolerant-bacteria-plant-growth-promotion-wheat-triticum-aestivum-l