02581nas a2200241 4500008004100000022001400041245014000055210006900195260007400264300001200338490000800350520166100358653002202019653002302041653001602064653002602080653002202106653001902128100002302147700002302170700001802193856012802211 2012 eng d a0014-233600aInheritance of resistance to root-lesion nematodes (Pratylenchus thornei and P. neglectus) in five doubled-haploid populations of wheat0 aInheritance of resistance to rootlesion nematodes Pratylenchus t aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcNOV a209-2190 v1883 a

Nematode species Pratylenchus thornei and P. neglectus are the two most important root-lesion nematodes affecting wheat (Triticum aestivum L.) and other grain crops in Australia. For practical plant breeding, it will be valuable to know the mode of inheritance of resistance and whether the same set of genes confer resistance to both species. We evaluated reactions to P. thornei and P. neglectus of glasshouse-inoculated plants of five doubled-haploid populations derived from five resistant synthetic hexpaloid wheat lines, each crossed to the susceptible Australian wheat cultivar Janz. For each cross we determined genetic variance, heritability and minimum number of effective resistance genes for each nematode species. Distributions of nematode numbers for both species were continuous for all doubled-haploid populations. Heritabilities were high and the resistances were controlled by 4-7 genes. There was no genetic correlation between resistance to P. thornei and to P. neglectus in four of the populations and a significant but low correlation in one. Therefore, resistances to P. thornei and to P. neglectus are probably inherited quantitatively and independently in four of these synthetic hexaploid wheat populations, with the possibility of at least one genetic factor contributing to resistance to both species in one of the populations. Parents with the greatest level of resistance will be the best to use as donor parents to adapted cultivars, and selection of resistance to both species in early generations will be optimal to carry resistance through successive cycles of inbreeding to produce resistant cultivars for release.

10aAegilops tauschii10aDisease resistance10aDurum wheat10aGermplasm development10aTriticum aestivum10aWheat breeding1 aThompson, John, P.1 aZwart, Rebecca, S.1 aButler, David uhttps://library.ncl.res.in/content/inheritance-resistance-root-lesion-nematodes-pratylenchus-thornei-and-p-neglectus-five-002695nas a2200265 4500008004100000022001400041245011400055210006900169260007400238300001400312490000700326520177900333653002302112653001202135653001402147653000902161653001002170100002202180700002302202700002102225700001802246700001702264700002502281856012302306 2012 eng d a1380-374300aMicrosatellite mapping identifies TTKST-effective stem rust resistance gene in wheat cultivars VL404 and Janz0 aMicrosatellite mapping identifies TTKSTeffective stem rust resis aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcDEC a1757-17650 v303 a

Wheat cultivar VL404 carries seedling resistance to Puccinia graminis f. sp. tritici pathotype TTKST. Monogenic segregation for seedling resistance was observed in a VL404/WL711 recombinant inbred line population and the resistance locus was temporarily designated SrVL. Bulked segregant analysis using Diversity Arrays Technology markers located SrVL on chromosome 2BL. Detailed simple sequence repeat mapping placed SrVL between gwm120 and wmc175, both at genetic distances of 3.3 cM. Based on adult plant responses of Janz and VL404 in India and Kenya, we expected these cultivars to carry the same gene against TTKST. A subset of Diamondbird/Janz doubled haploid (DH) population showed monogenic segregation, when tested against TTKST and the locus was temporarily named SrJNZ. SrVL-linked markers gwm120 and wmc175 flanked SrJNZ at a similar genetic distance, thereby confirming our hypothesis. Chromosome 2BL carries Sr9, Sr16 and Sr28. Sr9 is a multi-allelic locus and all known alleles of Sr9 and Sr16 are ineffective against TTKSK and its derivatives. A recombination value of 16.7 cM between Sr9g-linked stripe rust resistance gene Yr7 and SrJNZ in Diamondbird/Janz DH population suggested that SrJNZ is not an allele at the Sr9 locus. Based on comparison of published genetic distances between Lr13, Sr9, Sr28 and Sr16 with that observed in this study, we concluded SrVL and SrJNZ to be Sr28. This gene was contributed by a common parent Gabo, which also exhibited resistance against TTKST. Sr28-linked markers gwm120 and wmc175 confirmed the presence of this gene in a high proportion of Australian cultivars that showed stem rust resistance in Kenya. These markers can be used for marker-assisted pyramiding of Sr28 with other stem rust resistance genes.

10aDisease resistance10aMarkers10aStem rust10aUg9910aWheat1 aBansal, Urmil, K.1 aZwart, Rebecca, S.1 aBhavani, Sridhar1 aWanyera, Ruth1 aGupta, Vidya1 aBariana, Harbans, S. uhttps://library.ncl.res.in/content/microsatellite-mapping-identifies-ttkst-effective-stem-rust-resistance-gene-wheat-002233nas a2200313 4500008004100000022001400041245007500055210006900130260007400199300001200273490000700285520122400292653002301516653001401539653003001553653002701583653001001610100002601620700002001646700001601666700001701682700001501699700002101714700002001735700002201755700001801777700001801795856010601813 2013 eng d a1380-374300aMolecular mapping of leaf rust resistance gene Lr15 in hexaploid wheat0 aMolecular mapping of leaf rust resistance gene Lr15 in hexaploid aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcMAR a743-7470 v313 a

Leaf rust is a widespread and commonly occurring rust disease of wheat. Genetic resistance is the most economical method of reducing losses due to leaf rust. Lr15 has been shown to be present on wheat chromosome 2D and is reported to be a seedling resistance gene. However, tightly linked markers associated with Lr15 have not been reported to date. To identify molecular markers linked to Lr15, an F-2 mapping population of Thatcher x Thatcher-Lr15 was generated. Available wheat simple sequence repeat markers were utilized in parental screening and polymorphic markers were used to analyze the entire population of 221 plants. Phenotypic evaluations of the F-2-derived F-3 progenies with Puccinia triticina Eriks. pathotype 162A (93R15) confirmed the monogenic inheritance of Lr15. The linkage group representing chromosome 2DS was constructed at LOD 4.0 which revealed the closest flanking markers Xgwm4562 and Xgwm102 at a distance of 3.1 and 9.3 cM, respectively. Furthermore, utilization of these flanking markers in combination has successfully identified wheat lines with or without Lr15. These markers could potentially be useful in gene pyramiding with other genes to enhance rust resistance in wheat.

10aDisease resistance10aLeaf rust10aMarker-assisted selection10aSimple sequence repeat10aWheat1 aDholakia, Bhushan, B.1 aRajwade, A., V.1 aHosmani, P.1 aKhan, R., R.1 aChavan, S.1 aReddy, D., M. R.1 aLagu, Meena, D.1 aBansal, Urmil, K.1 aSaini, R., G.1 aGupta, V., S. uhttps://library.ncl.res.in/content/molecular-mapping-leaf-rust-resistance-gene-lr15-hexaploid-wheat-001867nas a2200241 4500008004100000022001400041245011200055210006900167260000800236300001400244490000800258520105500266653002301321653001801344653001201362653002101374653002201395653002001417100001901437700001801456700002001474856013101494 2020 eng d a0888-754300aPlant-pathogen interactions: microRNA-mediated trans-kingdom gene regulation in fungi and their host plants0 aPlantpathogen interactions microRNAmediated transkingdom gene re cSEP a3021-30350 v1123 a

MicroRNAs (miRNAs) have been prevalently studied in plants, animals, and viruses. However, recent studies show evidences of miRNA-like RNAs (milRNAs) in fungi as well. It is known that after successful infection, pathogens hijack the host machinery and use it for their own growth and multiplication. Alternatively, resistant plants can overcome the pathogen attack by a variety of mechanisms. Based on this prior knowledge, we computationally predicted milRNAs from 13 fungi, and identified their targets in transcriptomes of the respective fungi as well as their host plants. The expressions of the milRNAs and targets were confirmed using qRT-PCR. We found that plant miRNAs targeted fungal virulence genes, while fungal milRNAs targeted plant resistance genes; corroborating miRNA-mediated trans-kingdom gene regulation and the roles of miRNAs in plantpathogen interactions. Transgenic plants with miRNAs targeting fungal virulence genes, or anti-sense of fungal milRNAs, would be expected to be highly resistant to the fungal pathogens.

10aDisease resistance10aFungal miRNAs10aqRT-PCR10aResistance genes10aTarget prediction10aVirulence genes1 aMathur, Monika1 aNair, Aswathy1 aKadoo, Narendra uhttps://library.ncl.res.in/content/plant-pathogen-interactions-microrna-mediated-trans-kingdom-gene-regulation-fungi-and-their