01528nas a2200229   4500008004100000022001400041245005000055210004900105260007700154300001400231490000700245520082100252653001901073653001401092653002201106653001301128653001301141100002101154700002501175700001801200856008001218       2013                        eng d  a1439-422700aG-Quadruplexes as tools for synthetic biology0 aGQuadruplexes as tools for synthetic biology  aBOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANYbWILEY-V C H VERLAG GMBHcNOV  a2077-20810 v143 a<p>With the potential to engineer biological systems, synthetic biology is an emerging field that combines various disciplines of sciences. It encompasses combinations of DNA, RNA and protein modules for constructing desired systems and the rewiring of existing signalling networks. Despite recent advances, this field still lags behind in the artificial reconstruction of cellular processes, and thus demands new modules and switches to create genetic circuits. The widely characterised noncanonical nucleic acid secondary structures, G-quadruplexes are promising candidates to be used as biological modules in synthetic biology. Structural plasticity and functional versatility are significant G-quadruplex traits for its integration into a biological system and for diverse applications in synthetic circuits.</p>10aG-quadruplexes10aribozymes10asynthetic biology10atopology10azipcodes1 aAgarwala, Prachi1 aPandey, Satyaprakash1 aMaiti, Souvik  uhttps://library.ncl.res.in/content/g-quadruplexes-tools-synthetic-biology-001427nas a2200229   4500008004100000022001400041245012300055210006900178260000900247300001600256490000600272520062400278653001300902653001800915653002700933653001900960653002300979100002001002700002101022700002401043856013001067       2021                        eng d  a2365-654900aReplacement of loop residues in TBA by an abasic ethylene glycol spacer: effect on stability, structure and function**0 aReplacement of loop residues in TBA by an abasic ethylene glycol  cOCT   a10648-106500 v63 aThis article describes the synthesis of ethyleneglycol (E) phosphoramidite and its incorporation into the thrombin binding aptamer (TBA) sequence at loop positions. Circular dichroism (CD) study revealed no major disturbances in the secondary structure of TBA by the abasic E unit and the derived oligomers exhibited a typical antiparallel chair-like conformation similar to that of TBA. UV and CD spectroscopy, together with anti-coagulation and HPLC studies revealed that although nuclease stability was enhanced, and anti-coagulation reasonably good, the thermal stability of the quadruplexes was adversely affected.10aaptamers10aclotting time10aethylene glycol spacer10aG-quadruplexes10anuclease stability1 aWagh, Atish, A.1 aGhalawat, Monika1 aFernandes, Moneesha  uhttps://library.ncl.res.in/content/replacement-loop-residues-tba-abasic-ethylene-glycol-spacer-effect-stability-structure-and