01925nas a2200229   4500008004100000022001400041245013500055210006900190260007300259300001000332490000700342520105100349653002401400653002101424653001901445653002101464653002501485653002101510100001601531700002001547856012801567       2009                        eng d  a1381-117700aEffect of organic solvents on cell-bound penicillin V acylase activity of erwinia aroideae (DSMZ 30186): a permeabilization effect0 aEffect of organic solvents on cellbound penicillin V acylase act  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAY  a67-710 v573 a<p>Erwinia aroideae (DSMZ 30186) is a potential microbial culture to produce intracellular penicillin V acylase (PVA). The whole cell PVA activity was improved by permeabilization with various organic solvents. The cell-bound PVA activity showed an eightfold increase upon treatment with chloroform (5 mu L/mg(dry) (biomass)) for 10 min and diethyl ether (10 mu L/mg(dry) (biomass)) for 45 min. Hexane, toluene, ethyl acetate and dichloromethane enhanced the enzyme activity up to two-, six-, four- and two-fold, respectively; whereas, PVA activity declined drastically on permeabilization with acetone, pyridine and alcohols. The physicochemical properties of the organic solvents used for permeabilization were correlated with the change in activity. It was found that solvents with high hydrophobicity (log P &gt; 0.68) and lower dielectric constant (&lt; 9) were relatively effective in increasing PVA activity. These results allow systematic selection of suitable solvent for best performance. (c) 2008 Elsevier B.V. All rights reserved.</p>10adielectric constant10aErwinia aroideae10aHydrophobicity10aOrganic solvents10aPenicillin V acylase10aPermeabilization1 aKumar, Atul1 aPundle, Archana  uhttps://library.ncl.res.in/content/effect-organic-solvents-cell-bound-penicillin-v-acylase-activity-erwinia-aroideae-dsmz-001855nas a2200229   4500008004100000022001400041245009800055210006900153260009800222300001400320490000700334520097100341653001901312653002101331653003001352100002601382700002501408700001801433700002101451700002301472856013001495       2009                        eng d  a1042-714700aSynthesis and properties of new polybenzoxazines containing (substituted) cyclohexyl moieties0 aSynthesis and properties of new polybenzoxazines containing subs  aTHE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLANDbJOHN WILEY & SONS LTDcDEC  a1107-11130 v203 a<p>A series of new polybenzoxazines were synthesized based on diphenols containing (substituted) cyclohexyl moiety and were characterized by FT-IR, (1)H-NMR, and (13)C-NMR spectroscopy. These new benzoxazine monomers exhibited better processability with lower peak cure temperature and a wide cure controllable window (CCW) as manifested in differential scanning calorimetric analysis. The cure analysis was performed by FT-IR spectroscopy. Glass transition temperature of new polybenzoxazines varied from 170 to 205 degrees C. The cyclohexyl bridge groups facilitated ring opening, resulting in polymer with improved thermal stability in comparison to bisphenol A-based benzoxazine as assessed by the various thermal analyses. The water contact angles of polybenzoxazines containing (substituted) cyclohexyl moieties were higher than that of bisphenol A-based polybenzoxazine, implying their higher hydrophobicity. Copyright (C) 2009 John Wiley &amp; Sons, Ltd.</p>10aHydrophobicity10apolybenzoxazines10athermally stable polymers1 aKumar, K., S. Santhos1 aNair, C., P. Reghuna1 aNinan, K., N.1 aKulkarni, A., D.1 aWadgaonkar, P., P.  uhttps://library.ncl.res.in/content/synthesis-and-properties-new-polybenzoxazines-containing-substituted-cyclohexyl-moieties-002329nas a2200301   4500008004100000022001400041245003300055210003200088260008000120300001400200490000800214520144900222653001401671653002501685653001901710653002301729653002001752100001801772700001601790700001701806700002001823700003201843700002101875700002401896700001701920700002001937856007001957       2012                        eng d  a0027-842400aRapid self-healing hydrogels0 aRapid selfhealing hydrogels  a2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USAbNATL ACAD SCIENCEScMAR  a4383-43880 v1093 a<p>Synthetic materials that are capable of autonomous healing upon damage are being developed at a rapid pace because of their many potential applications. Despite these advancements, achieving self-healing in permanently cross-linked hydrogels has remained elusive because of the presence of water and irreversible cross-links. Here, we demonstrate that permanently cross-linked hydrogels can be engineered to exhibit self-healing in an aqueous environment. We achieve this feature by arming the hydrogel network with flexible-pendant side chains carrying an optimal balance of hydrophilic and hydrophobic moieties that allows the side chains to mediate hydrogen bonds across the hydrogel interfaces with minimal steric hindrance and hydrophobic collapse. The self-healing reported here is rapid, occurring within seconds of the insertion of a crack into the hydrogel or juxtaposition of two separate hydrogel pieces. The healing is reversible and can be switched on and off via changes in pH, allowing external control over the healing process. Moreover, the hydrogels can sustain multiple cycles of healing and separation without compromising their mechanical properties and healing kinetics. Beyond revealing how secondary interactions could be harnessed to introduce new functions to chemically crosslinked polymeric systems, we also demonstrate various potential applications of such easy-to-synthesize, smart, self-healing hydrogels.</p>10aadhesives10abiomimetic materials10aHydrophobicity10amolecular dynamics10asmart materials1 aPhadke, Ameya1 aZhang, Chao1 aArman, Bedri1 aHsu, Cheng-Chih1 aMashelkar, Raghunath, Anant1 aLele, Ashish, K.1 aTauber, Michael, J.1 aArya, Gaurav1 aVarghese, Shyni  uhttps://library.ncl.res.in/content/rapid-self-healing-hydrogels-002020nas a2200265   4500008004100000022001400041245010300055210006900158260007300227300001200300490000800312520108200320653001501402653001701417653001901434653001201453653001201465100003101477700002401508700001901532700001901551700003001570700002501600856012901625       2014                        eng d  a0927-776500aComparison of polymer induced and solvent induced trypsin denaturation: the role of hydrophobicity0 aComparison of polymer induced and solvent induced trypsin denatu  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcAPR  a201-2050 v1163 a<p>Trypsin adsorption from aqueous buffer by various copolymers of allyl glycidyl ether-ethylene glycol dimethacrylate (AGE-EGDM) copolymer with varying crosslink density increases with increasing crosslink density and the effect slowly wears off after reaching a plateau at 50% crosslink density. The copolymer with 25% crosslink density was reacted with different amines with alkyl/aryl side chains to obtain a series of copolymers with 1,2-amino alcohol functional groups and varying hydrophobicity. Trypsin binding capacity again increases with hydrophobicity of the reacting amine and a good correlation between logP(octanol) of the amine and protein binding is observed. The bound trypsin is denatured to the extent of 90% in spite of the presence of hydrophilic hydroxyl and amino groups. The behavior was comparable to that in mixtures of aqueous buffer and water-miscible organic co-solvents where the solvent concentration required to deactivate 50% of the enzyme (C-50) is dependent on logP(octanol) of the co-solvent. (C) 2014 Elsevier B.V. All rights reserved.</p>10aAdsorption10aDenaturation10aHydrophobicity10aPolymer10aTrypsin1 aJasti, Lakshmi, Swarnalath1 aFadnavis, Nitin, W.1 aAddepally, Uma1 aDaniels, Siona1 aDeokar, Sarika, Babasaheb1 aPonrathnam, Surendra  uhttps://library.ncl.res.in/content/comparison-polymer-induced-and-solvent-induced-trypsin-denaturation-role-hydrophobicity-002463nas a2200241   4500008004100000022001400041245019400055210006900249260000800318300000800326490000800334520156100342653002601903653001901929653001501948653002901963653001401992100001802006700001502024700002802039700002602067856012802093       2022                        eng d  a0302-893300aIsoeugenol affects expression pattern of conidial hydrophobin gene RodA and transcriptional regulators MedA and SomA responsible for adherence and biofilm formation in Aspergillus fumigatus0 aIsoeugenol affects expression pattern of conidial hydrophobin ge  cAPR  a2140 v2043 a<p>
	Aspergillus fumigatus is one of the major pathogenic fungal species, causing life-threatening infections. Due to a limited spectrum of available antifungals, exploration of new drug targets as well as potential antifungal molecules has become pertinent. Rodlet layer plays an important role in adherence of fungal conidia to hydrophobic cell surfaces in host, which also leads to A. fumigatus biofilm formation, contributing factor to fungal pathogenicity. From decades, natural sources have been known for the development of new active molecules. The present study investigates effect of isoeugenol on genes responsible for hydrophobins (RodA), adhesion as well as biofilm formation (MedA and SomA) of A. fumigatus. Minimum inhibitory concentrations (MIC and IC50) of isoeugenol against A. fumigatus were determined using broth microdilution assay. The IC50 results showed reduced hydrophobicity and biofilm formation as well as eradication after treatment with the compound and electron micrograph data corroborated these findings. The qRT-PCR showed a significant downregulation of genes RodA, MedA, SomA and pksP involved in hydrophobicity and biofilm formation. SwissADME studies potentiated drug-like propensity for isoeugenol which formed four hydrogen bonds with low binding energy (- 4.54 kcal/mol) at the catalytic site of RodA protein studied via AutoDock4. Hence, the findings conclude that isoeugenol inhibits conidial hydrophobicity and biofilm formation of A. fumigatus and further investigations are warranted in this direction.</p>
10aAspergillus fumigatus10aHydrophobicity10aIsoeugenol10aTranscription regulators10aVirulence1 aGupta, Lovely1 aSen, Pooja1 aBhattacharya, Asish, K.1 aVijayaraghavan, Pooja  uhttps://library.ncl.res.in/content/isoeugenol-affects-expression-pattern-conidial-hydrophobin-gene-roda-and-transcriptional