02373nas a2200277 4500008004100000022001400041245014600055210006900201260007100270300001600341490000600357520136000363653001301723653002501736653001301761653001201774653002301786653001801809100002901827700002601856700001901882700001701901700002701918700002201945856012801967 2014 eng d a1944-824400aSol-gel assisted fabrication of collagen hydrolysate composite scaffold: a novel therapeutic alternative to the traditional collagen scaffold0 aSolgel assisted fabrication of collagen hydrolysate composite sc a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcSEP a15015-150250 v63 a

Collagen is one of the most widely used biomaterial for various biomedical applications. In this Research Article, we present a novel approach of using collagen hydrolysate, smaller fragments of collagen, as an alternative to traditionally used collagen scaffold. Collagen hydrolysate composite scaffold (CHCS) was fabricated with sol-gel transition procedure using tetraethoxysilane as the silica precursor. CHCS exhibits porous morphology with pore sizes varying between 380 and 780 mu m. Incorporation of silica conferred CHCS with controlled biodegradation and better water uptake capacity. Notably, 3T3 fibroblast proliferation was seen to be significantly better under CHCS treatment when compared to treatment with collagen scaffold. Additionally, CHCS showed excellent antimicrobial activity against the wound pathogens Staphylococcus aureus, Bacillus subtilis, and Escherichia coli due to the inherited antimicrobial activity of collagen hydrolysate. In vivo wound healing experiments with full thickness excision wounds in rat model demonstrated that wounds treated with CHCS showed accelerated healing when compared to wounds treated with collagen scaffold. These findings indicate that the CHCS scaffold from collagen fragments would be an effective and affordable alternative to the traditionally used collagen structural biomaterials.

10acollagen10acollagen hydrolysate10ascaffold10asol-gel10atissue engineering10awound Healing1 aRamadass, Satiesh, Kumar1 aPerumal, Sathiamurthi1 aGopinath, Arun1 aNisal, Anuya1 aSubramanian, Saravanan1 aMadhan, Balaraman uhttps://library.ncl.res.in/content/sol-gel-assisted-fabrication-collagen-hydrolysate-composite-scaffold-novel-therapeutic-002327nas a2200253 4500008004100000022001400041245008200055210006900137260000900206300001200215490000700227520152500234653001801759653001901777653001701796653001501813653002101828653001801849100002201867700002201889700002201911700002701933856011301960 2015 eng d a0928-493100aBioactive thermoresponsive polyblend nanofiber formulations for wound healing0 aBioactive thermoresponsive polyblend nanofiber formulations for  cMAR  a126-1370 v483 a

The rationale of this work is to develop new bioactive thermoresponsive polyblend nanofiber formulations for wound healing (topical). Various polymer compositions of thermoresponsive, poly(N-isopropylacrylamide), egg albumen and poly(epsilon-caprolactone) blend solutions with and without a drug [gatifloxacin hydrochloride, Gati] were prepared. Non-woven nanofibers of various compositions were fabricated using an electrospinning technique. The morphology of the nanofibers was analyzed by an environmental scanning electron microscope. The morphology was influenced by the concentration of polymer, drug, and polymer blend composition. Fourier transform infrared spectroscopy analysis showed the shift in bands due to hydrogen ion interactions between polymers and drug. Thermogram of PNIPAM/PCL/EA with Gati recorded a shift in lower critical solution temperature (LCST) and glass transition temperature (T-g) of PNIPAM. Similarly T-g and melting temperature (T-m) of PCL were shifted. X-ray diffraction patterns recorded a decrease in the crystalline state of PCL nanofibers and transformed crystalline drug to an amorphous state. In vitro release study of nanofibers with Gati showed initial rapid release up to 10 h, followed by slow and controlled release for 696 h (29 days). Nanofiber mats with Gati exhibited antibacterial properties to Staphylococcus aureus, supported suitable controlled drug release with in vitro cell viability and in vivo wound healing. (C) 2014 Elsevier B.V. All rights reserved.

10aAntibacterial10aCell viability10adrug release10ananofibers10athermoresponsive10awound Healing1 aPawar, Mahesh, D.1 aRathna, G., V. N.1 aAgrawal, Shubhang1 aKuchekar, Bhanudas, S. uhttps://library.ncl.res.in/content/bioactive-thermoresponsive-polyblend-nanofiber-formulations-wound-healing02821nas a2200265 4500008004100000022001400041245014400055210006900199260007300268300001200341490000700353520184300360653001302203653000902216653002002225653001802245653001802263100002802281700002002309700002302329700001802352700002702370700003002397856012802427 2016 eng d a0928-493100aAmelioration of excision wounds by topical application of green synthesized, formulated silver and gold nanoparticles in albino Wistar rats0 aAmelioration of excision wounds by topical application of green  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAY a293-3000 v623 a

Wound healing, a complex biological process, has attained a lot of attention as dermatologists are primarily interested in stimulated wound closure without formation of scar or a faint scar. The recent upsurgence of nanotechnology has provided novel therapeutic materials in the form of silver and gold nanoparticles which accelerate the wound healing process. The effect of formulated nanoparticles using Coleus forskohlii root extract (green synthesized) has been tried out for ameliorating full thickness excision wounds in albino Wistar male rats. The evaluation of in vivo activity of nanoparticles in wound healing was carried out on open wounds made by excision on the dorsal sides of albino Wistar rats under anesthesia, and the healing of the wounds was assessed. Histological aspects of the healing process were studied by a HE (Hematoxylin and Eosin) staining method to assess various degrees of re-epithelialization and the linear alignment of the granulation tissue whereas Van Gieson's histochemical staining was performed to observe collagen fibers. The healing action shown by the formulated nanoparticles was remarkable during the early stages of wound healing, which resulted in the substantial reduction of the whole healing period. Topical application of formulated gold nanoparticles was found to be more effective in suppressing inflammation and stimulating re-epithelialization compared to silver nanoparticles during the healing process. The results throw light on the amelioration of excision wounds using nanoparticles which could be a novel therapeutic way of improving wound healing in clinical practice. The mechanism of advanced healing action of both types of nanoparticles could be due to their antimicrobial, antioxidant and anti-inflammatory properties. (C) 2016 Elsevier B.V. All rights reserved.

10acollagen10aGold10agreen synthesis10aNanopraticles10awound Healing1 aNaraginti, Saraschandra1 aKumari, Lakshmi1 aDas, Raunak, Kumar1 aSivakumar, A.1 aPatil, Sagar, Hindurao1 aAndhalkar, Vaibhav, Vilas uhttps://library.ncl.res.in/content/amelioration-excision-wounds-topical-application-green-synthesized-formulated-silver-and01952nas a2200253 4500008004100000022001400041245010000055210006900155260000800224300001200232490000700244520113400251653002101385653001601406653001201422653001701434653001801451100001701469700002601486700001901512700002001531700002101551856012601572 2019 eng d a0973-129600aFormulation and evaluation of wound healing activity of sophorolipid-sericin gel in wistar rats0 aFormulation and evaluation of wound healing activity of sophorol cAPR a123-1270 v153 a

Sericin is a useful by-product of silk processing and is resistant to oxidation and ultraviolet and can absorb and release moisture easily. Sericin has biological activities such as antibacterial, antioxidant, and tyrosinase inhibition. Sophorolipids are microbial extracellular glycolipids produced by resting cells of Candida bombicola. Sophorolipids show excellent skin compatibility and also have antibacterial property. In this study, we have developed a novel formulation consisting of sericin and sophorolipid with calcium alginate as a binding agent. Since both the ingredients are biocompatible and biodegradable, the formulation was tested for wound healing in Wistar rats. A commercial ointment povidone was used as control. The animal group, treated with sericin and sophorolipid cream, showed fast contraction, rapid closure, and healing when compared with control and commercial ointment. These observations were validated with histopathological studies where more fibroblast proliferation, angiogenesis, and keratinization were observed. This is a green, cost-effective formulation for fast wound healing.

10aBiocompatibility10aformulation10asericin10aSophorolipid10awound Healing1 aMore, Snehal1 aKoratkar, Santosh, S.1 aKadam, Navnath1 aAgawane, Sachin1 aPrabhune, Asmita uhttps://library.ncl.res.in/content/formulation-and-evaluation-wound-healing-activity-sophorolipid-sericin-gel-wistar-rats02609nas a2200325 4500008004100000022001400041245011700055210006900172260000800241300001400249490000600263520156100269653002701830653001201857653002201869653001501891653001801906100002301924700001601947700001701963700002301980700001802003700001902021700001602040700002202056700002502078700002502103700002402128856013102152 2022 eng d a2637-610500aAlgal biomass-loaded hydrogel scaffolds as a biomimetic platform with antibacterial and wound healing activities0 aAlgal biomassloaded hydrogel scaffolds as a biomimetic platform  cAUG a5800-58120 v43 a

The confluence of hydrogel scaffolds and dried algal biomass (AB), consisting of all the bioactive compounds, offers the possibility to facilitate wound healing while simultaneously instilling antibacterial benefits. For this purpose, a single-step synthesis of algal (Chlorella sorokiniana) biomass-loaded hydrogel scaffolds (AHS) was achieved. C. sorokiniana has been used in different areas for several years and has proved attractive to the pharmaceutical and cosmetic industries. Of note, the presence of phytochemicals and various bioactive compounds provides an added health benefit. Hitherto, we report AHS with accelerated wound healing along with potent anti-inflammatory and antibacterial properties. AHS consisting of different concentrations of AB was applied for 14 days on excisional wounds in mice. Microscopic analyses, assessment of proinflammatory and anti-inflammatory cytokines, and histological studies were performed to investigate wound healing. These scaffolds were extensively characterized and studied using Fourier transform infrared, X-ray diffraction, Raman, atomic force microscopy, transmission electron microscopy, scanning electron microscopy, swelling, rheological, thermal, and mechanical analyses. AHS have excellent biocompatibility in addition to significant antibacterial activity against Escherichia coli (99%) and Staphylococcus aureus (98%). We believe that the as-synthesized AHS have the potential to broaden the arsenal of more effective wound healing processes along with antibacterial activities.

10aantibacterial activity10aBiomass10ahydrogel scaffold10amicroalgae10awound Healing1 aAgarwal, Aakanksha1 aKumar, Arun1 aGarg, Piyush1 aChakraborty, Arnab1 aVerma, Ranjan1 aSarwat, Maryam1 aGupta, Ajay1 aSasmal, Pijus, K.1 aVerma, Yogesh, Kumar1 aChowdhury, Chiranjit1 aMukherjee, Monalisa uhttps://library.ncl.res.in/content/algal-biomass-loaded-hydrogel-scaffolds-biomimetic-platform-antibacterial-and-wound-healing02661nas a2200241 4500008004100000022001400041245013400055210006900189260000800258300001100266490000700277520181500284653001902099653001302118653001502131653002302146653001802169100002902187700002102216700001902237700003402256856012902290 2022 eng d a1773-224700aBioinspired hyaluronic acid based nanofibers immobilized with 3, 4-difluorobenzylidene curcumin for treating bacterial infections0 aBioinspired hyaluronic acid based nanofibers immobilized with 3  cAUG a1034800 v743 a

Curcumin (Cur) is a natural polyphenol with multifaceted pharmacological functions, exploited extensively for biomedical applications. Traditionally curcumin is being used as an antimicrobial agent. However, to improvise the pharmacological properties, it is being modified synthetically. One of such modified Cur is 3, 4- difluorobenzylidene curcumin (CDF) which is aimed for enhancing the anti-cancer properties. Though there are reports on the studies of anti-cancer properties involving CDF, the anti-bacterial property is yet to be demonstrated. Accordingly, in our studies, we prepared bioinspired hyaluronic acid blends immobilized with CDF and fabricated non-woven nanofiber mats. These nanofiber mats were characterized and demonstrated in vitro cell culture studies, which involved cell viability, hemolysis, anti-bacterial and cell scratch assay to understand their efficacy in treating bacteria. The molecular docking studies of CDF and Cur were performed on the dihydrofolate reductase (DHFR) enzyme receptor, which is an essential protein of S.auerus (Staphylococcus aureus). The results of MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay, and hemolysis of the respective nanofiber mats with Cur and CDF showed non-toxicity and were compatible with blood cells. Further, the cell proliferation and adherence recorded >60% fibroblast cells for the nanofiber mats. The anti-bacterial property of Cur and CDF was similar. The in vitro release studies for the respective Cur and CDF loaded nanofiber mats recorded a release of 25 and 37%, respectively. From these studies, we concluded that the CDF sustained its antibacterial property in addition to the improved anti-cancer property; hence CDF being synergetic, it will have a better scope in cancer therapy.

10aanti-bacterial10acurcumin10ananofibers10atissue engineering10awound Healing1 aPatel, Pratikshkumar, R.1 aSingam, Amarnath1 aIyer, Arun, K.1 aGundloori, Rathna, Venkata Na uhttps://library.ncl.res.in/content/bioinspired-hyaluronic-acid-based-nanofibers-immobilized-3-4-difluorobenzylidene-curcumin02126nas a2200265 4500008004100000022001400041245009700055210006900152260000800221300001100229490000700240520127600247653002101523653001501544653001401559653001701573653001801590100002101608700002401629700001901653700001801672700001701690700002601707856012701733 2025 eng d a1616-518700aEngineered silk-dressing for acceleratedwound healing: biocompatibility and efficacy studies0 aEngineered silkdressing for acceleratedwound healing biocompatib cNOV ae003230 v253 a

Advanced wound care dressings are essential for improving clinical outcomes. The present study investigates the wound management potential of a unique dressing fabricated from silk proteins. The dressing was characterized for its physical and structural properties, including surface texture, porosity, fluid absorption capacity, and moisture vapor transmission rate. These parameters have been found to be critical for optimal wound healing. In vivo full thickness wound healing studies in a rat model validated the efficacy of the Silk-dressing compared to conventional cotton gauze and commercial polyurethane foam dressings. Histopathological analysis confirmed improved re-epithelialization, collagen deposition, angiogenesis, and formation of secondary follicles. Key advantages of Silk-dressing included non-adherence, absorption of exudate, maintenance of optimal moisture at wound site and acceleratedwound closure. Biocompatibility studies were also conducted in accordance with ISO 10993 guidelines, demonstrating no cytotoxicity, irritation, sensitization, or pyrogenicity. These findings highlight the potential of this uniquely designed Silk-dressing as a superior alternative for wound management, with a potential to improve clinical outcomes.

10aBiocompatibility10abiopolymer10aISO 1099310aSilk protein10awound Healing1 aDeshpande, Rucha1 aPillai, Lakshmi, R.1 aSayyad, Raeesa1 aShukla, Swati1 aNisal, Anuya1 aVenugopalan, Premnath uhttps://library.ncl.res.in/content/engineered-silk-dressing-acceleratedwound-healing-biocompatibility-and-efficacy-studies02793nas a2200301 4500008004100000022001400041245009500055210006900150260000900219300001100228490000800239520180900247653001302056653002202069653003102091653002402122653001402146653001802160100002702178700002902205700002502234700002302259700001802282700002902300700001602329700002302345856012302368 2025 eng d a0378-517300aStimulant immobilized bioactive film of functionalized egg albumin blend for wound healing0 aStimulant immobilized bioactive film of functionalized egg album cSEP  a1258960 v6823 a

Wound healing materials with advanced properties that facilitates higher collagen deposition, improved angiogenesis and quick tissue regeneration are crucial for clinical wound management. To meet the requirement, for the first time, our studies focus on engineering bio-originated natural materials, which are tested in combination with the active agents, ascorbic acid (AA), a stimulant and metronidazole (Mtz), an anti-microbial drug. Accordingly, a dual drug (AA, and Mtz) loaded film of functionalized egg albumin (FEA)-poly(vinyl alcohol) (PVA) was fabricated following the solution casting method. The film was characterized for its morphology and physicochemical properties using various analytical tools. The potential of the film as a wound healing material was evaluated, by in vitro drug release, degradation, cell viability, antimicrobial studies, in vivo wound healing, and histopathological analyses. In vitro degradation studies confirmed their degradability in enzymatic and soil burial conditions. Cytotoxicity studies demonstrated their non-toxicity, and the antimicrobial investigations showcased that the material was antibacterial. On the 14th day, the wound closure percentage of the wound induced control group, GI (without treatment) was notably higher at 95 % compared to the test formulation group, GV [FEA-PVA (30/70 w/w) loaded with Mtz and AA (10 % w/w of the total polymer weight), respectively], which exhibited a wound closure of 83 %. Furthermore, the histopathological examinations revealed that the inner wound healing in GV was comparatively better than in GI in terms of angiogenesis, epidermal remodeling, higher collagen deposition, coherency, and tissue regeneration. Consequently, the formulated film can be deemed a suitable wound dressing material.

10acollagen10aDual drug release10aFunctionalized egg albumin10aPolymer blend films10aStimulant10awound Healing1 aGautam, Tripurari, Rao1 aPatel, Pratikshkumar, R.1 aSingam, Amarnath, R.1 aJagtap, Ashish, S.1 aDesai, Prasad1 aGundloori, Rathna, V. N.1 aPawar, Anil1 aDastager, Syed, G. uhttps://library.ncl.res.in/content/stimulant-immobilized-bioactive-film-functionalized-egg-albumin-blend-wound-healing