01418nas a2200289 4500008004100000022001400041245004400055210004300099260006900142300001200211490000800223520052500231653002000756653002100776653001300797653002600810653002800836100002600864700002800890700002300918700002300941700002600964700001800990700001901008700002301027856007801050 2008 eng d a0022-113900aPractical syntheses of 4-fluoroprolines0 aPractical syntheses of 4fluoroprolines aPO BOX 564, 1001 LAUSANNE, SWITZERLANDbELSEVIER SCIENCE SAcSEP a781-7840 v1293 a

4-Fluoroprolines are among the most useful nonnatural amino acids in chemical biology. Here, practical routes are reported for the synthesis of the 2S, 4R, 2S, 4S, and 2R,4S diastereomers of 4-fluoroproline. Each route starts with (2S,4R)-4-hydroxyproline, which is a prevalent component of collagen and hence readily available, and uses a fluoride salt to install the fluoro group. Hence, the routes provide process-scale access to these useful nonnatural amino acids. (c) 2008 Elsevier B.V. All rights reserved.

10a4-Fluoroproline10a4-Hydroxyproline10acollagen10aNonnatural amino acid10aProcess-scale synthesis1 aChorghade, Mukund, S.1 aMohapatra, Debendra, K.1 aSahoo, Gokarneswar1 aGurjar, Mukund, K.1 aMandlecha, Manish, V.1 aBhoite, Nitin1 aMoghe, Santosh1 aRaines, Ronald, T. uhttps://library.ncl.res.in/content/practical-syntheses-4-fluoroprolines-002373nas a2200277 4500008004100000022001400041245014600055210006900201260007100270300001600341490000600357520136000363653001301723653002501736653001301761653001201774653002301786653001801809100002901827700002601856700001901882700001701901700002701918700002201945856012801967 2014 eng d a1944-824400aSol-gel assisted fabrication of collagen hydrolysate composite scaffold: a novel therapeutic alternative to the traditional collagen scaffold0 aSolgel assisted fabrication of collagen hydrolysate composite sc a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcSEP a15015-150250 v63 a

Collagen is one of the most widely used biomaterial for various biomedical applications. In this Research Article, we present a novel approach of using collagen hydrolysate, smaller fragments of collagen, as an alternative to traditionally used collagen scaffold. Collagen hydrolysate composite scaffold (CHCS) was fabricated with sol-gel transition procedure using tetraethoxysilane as the silica precursor. CHCS exhibits porous morphology with pore sizes varying between 380 and 780 mu m. Incorporation of silica conferred CHCS with controlled biodegradation and better water uptake capacity. Notably, 3T3 fibroblast proliferation was seen to be significantly better under CHCS treatment when compared to treatment with collagen scaffold. Additionally, CHCS showed excellent antimicrobial activity against the wound pathogens Staphylococcus aureus, Bacillus subtilis, and Escherichia coli due to the inherited antimicrobial activity of collagen hydrolysate. In vivo wound healing experiments with full thickness excision wounds in rat model demonstrated that wounds treated with CHCS showed accelerated healing when compared to wounds treated with collagen scaffold. These findings indicate that the CHCS scaffold from collagen fragments would be an effective and affordable alternative to the traditionally used collagen structural biomaterials.

10acollagen10acollagen hydrolysate10ascaffold10asol-gel10atissue engineering10awound Healing1 aRamadass, Satiesh, Kumar1 aPerumal, Sathiamurthi1 aGopinath, Arun1 aNisal, Anuya1 aSubramanian, Saravanan1 aMadhan, Balaraman uhttps://library.ncl.res.in/content/sol-gel-assisted-fabrication-collagen-hydrolysate-composite-scaffold-novel-therapeutic-002821nas a2200265 4500008004100000022001400041245014400055210006900199260007300268300001200341490000700353520184300360653001302203653000902216653002002225653001802245653001802263100002802281700002002309700002302329700001802352700002702370700003002397856012802427 2016 eng d a0928-493100aAmelioration of excision wounds by topical application of green synthesized, formulated silver and gold nanoparticles in albino Wistar rats0 aAmelioration of excision wounds by topical application of green  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAY a293-3000 v623 a

Wound healing, a complex biological process, has attained a lot of attention as dermatologists are primarily interested in stimulated wound closure without formation of scar or a faint scar. The recent upsurgence of nanotechnology has provided novel therapeutic materials in the form of silver and gold nanoparticles which accelerate the wound healing process. The effect of formulated nanoparticles using Coleus forskohlii root extract (green synthesized) has been tried out for ameliorating full thickness excision wounds in albino Wistar male rats. The evaluation of in vivo activity of nanoparticles in wound healing was carried out on open wounds made by excision on the dorsal sides of albino Wistar rats under anesthesia, and the healing of the wounds was assessed. Histological aspects of the healing process were studied by a HE (Hematoxylin and Eosin) staining method to assess various degrees of re-epithelialization and the linear alignment of the granulation tissue whereas Van Gieson's histochemical staining was performed to observe collagen fibers. The healing action shown by the formulated nanoparticles was remarkable during the early stages of wound healing, which resulted in the substantial reduction of the whole healing period. Topical application of formulated gold nanoparticles was found to be more effective in suppressing inflammation and stimulating re-epithelialization compared to silver nanoparticles during the healing process. The results throw light on the amelioration of excision wounds using nanoparticles which could be a novel therapeutic way of improving wound healing in clinical practice. The mechanism of advanced healing action of both types of nanoparticles could be due to their antimicrobial, antioxidant and anti-inflammatory properties. (C) 2016 Elsevier B.V. All rights reserved.

10acollagen10aGold10agreen synthesis10aNanopraticles10awound Healing1 aNaraginti, Saraschandra1 aKumari, Lakshmi1 aDas, Raunak, Kumar1 aSivakumar, A.1 aPatil, Sagar, Hindurao1 aAndhalkar, Vaibhav, Vilas uhttps://library.ncl.res.in/content/amelioration-excision-wounds-topical-application-green-synthesized-formulated-silver-and02258nas a2200277 4500008004100000022001400041245009400055210006900149260000800218300000800226490000800234520130900242653003301551653001301584653001201597653003001609653001401639100003801653700002701691700002301718700003701741700002401778700002701802700003401829856011701863 2025 eng d a0175-759800aLow-cost gelatin/collagen scaffolds for bacterial growth in bioreactors for biotechnology0 aLowcost gelatincollagen scaffolds for bacterial growth in biorea cMAY a1130 v1093 a

A wide array of pharmaceutical and industrial products available in today's market stems from bioreactors. Meeting the escalating demand for these products necessitates significant enhancements in biotechnological processes. This study focuses on developing cost-effective scaffolds designed explicitly for use within bioreactors, employing commonly used polymers such as gelatin and collagen. Bacterial proliferation assays involving Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa were conducted to assess the effectiveness of these scaffolds. The scaffolds were produced by electrospinning polymeric solutions with varying concentrations of gelatin and collagen and were characterized using scanning electron microscopy, Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetric analysis. Results revealed that scaffolds with 15% gelatin increased the 24-h proliferation of S. aureus, P. aeruginosa, and E. coli by 52%, 35%, and 20%, respectively. In the case of E. coli, scaffolds with lower gelatin concentrations (1-10%) were more effective, leading to 35-55% proliferation growth. These findings highlight the potential application of gelatin/collagen scaffolds in fabricating industrial products derived from these bacteria.

10aBacterial cell proliferation10acollagen10agelatin10aPolymeric electrospinning10ascaffolds1 aPompa-Monroy, Daniella, Alejandra1 aVera-Graziano, Ricardo1 aDastager, Syed, G.1 aPerez-Gonzalez, Graciela, Lizeth1 aBogdanchikova, Nina1 aIglesias, Ana, Leticia1 aVillarreal-Gomez, Luis, Jesus uhttps://library.ncl.res.in/content/low-cost-gelatincollagen-scaffolds-bacterial-growth-bioreactors-biotechnology02793nas a2200301 4500008004100000022001400041245009500055210006900150260000900219300001100228490000800239520180900247653001302056653002202069653003102091653002402122653001402146653001802160100002702178700002902205700002502234700002302259700001802282700002902300700001602329700002302345856012302368 2025 eng d a0378-517300aStimulant immobilized bioactive film of functionalized egg albumin blend for wound healing0 aStimulant immobilized bioactive film of functionalized egg album cSEP  a1258960 v6823 a

Wound healing materials with advanced properties that facilitates higher collagen deposition, improved angiogenesis and quick tissue regeneration are crucial for clinical wound management. To meet the requirement, for the first time, our studies focus on engineering bio-originated natural materials, which are tested in combination with the active agents, ascorbic acid (AA), a stimulant and metronidazole (Mtz), an anti-microbial drug. Accordingly, a dual drug (AA, and Mtz) loaded film of functionalized egg albumin (FEA)-poly(vinyl alcohol) (PVA) was fabricated following the solution casting method. The film was characterized for its morphology and physicochemical properties using various analytical tools. The potential of the film as a wound healing material was evaluated, by in vitro drug release, degradation, cell viability, antimicrobial studies, in vivo wound healing, and histopathological analyses. In vitro degradation studies confirmed their degradability in enzymatic and soil burial conditions. Cytotoxicity studies demonstrated their non-toxicity, and the antimicrobial investigations showcased that the material was antibacterial. On the 14th day, the wound closure percentage of the wound induced control group, GI (without treatment) was notably higher at 95 % compared to the test formulation group, GV [FEA-PVA (30/70 w/w) loaded with Mtz and AA (10 % w/w of the total polymer weight), respectively], which exhibited a wound closure of 83 %. Furthermore, the histopathological examinations revealed that the inner wound healing in GV was comparatively better than in GI in terms of angiogenesis, epidermal remodeling, higher collagen deposition, coherency, and tissue regeneration. Consequently, the formulated film can be deemed a suitable wound dressing material.

10acollagen10aDual drug release10aFunctionalized egg albumin10aPolymer blend films10aStimulant10awound Healing1 aGautam, Tripurari, Rao1 aPatel, Pratikshkumar, R.1 aSingam, Amarnath, R.1 aJagtap, Ashish, S.1 aDesai, Prasad1 aGundloori, Rathna, V. N.1 aPawar, Anil1 aDastager, Syed, G. uhttps://library.ncl.res.in/content/stimulant-immobilized-bioactive-film-functionalized-egg-albumin-blend-wound-healing