01403nas a2200193   4500008004100000022001400041245005800055210005800113260007300171300001000244490000800254520078000262653001701042653000801059653001701067100002101084700001401105856009001119       2005                        eng d  a0378-109700aPHB synthase from Streptomyces aureofaciens NRRL 22090 aPHB synthase from Streptomyces aureofaciens NRRL 2209  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJAN  a13-180 v2423 a<p>An similar to4.9 kb Sau3A I genomic DNA fragment from the Streptomyces aureofaciens NRRL 2209 aiding in the biosynthesis of PHB in recombinant Escherichia coli has been sequenced and analysed for phaC gene. The putative phaC(sa) gene of 2 kb is 79.1% GC rich and encodes a 63.5 kDa protein. It expressed under its own promoter and significant PHA synthase activity was detected in the recombinant E coli. This is the first putative PHA synthase gene reported from a Streptomyces sp. with serine as the active nucleophile in the conserved lipase box. The phaC(sa) was found in close proximity to a regulatory gene, which apparently regulated the phaC expression. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.</p>10aPHA synthase10aPHB10aStreptomyces1 aRamachander, TVN1 aRawal, SK  uhttps://library.ncl.res.in/content/phb-synthase-streptomyces-aureofaciens-nrrl-2209-002407nas a2200253   4500008004100000022001400041245016000055210006900215260007700284300001200361490000700373520141300380653002301793653001701816653001701833653003901850100002301889700002501912700002301937700002401960700002001984700001802004856013102022       2013                        eng d  a0038-905600aOptimization of the production of a maltooligosaccharides producing amylase from the alkalophilic streptomyces lonarensis strain NCL 716 using SVR modeling0 aOptimization of the production of a maltooligosaccharides produc  aBOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANYbWILEY-V C H VERLAG GMBHcJAN  a179-1850 v653 a<p>The Streptomyces lonarensis strain NCL 716 hydrolyses starch to produce a mixture of maltotriose (G3) and maltotetraose (G4) along with maltose (G2). The objective of the present work was to determine an optimum cost effective media composition for the production of a-amylase from this strain. The most influential factor was found to be starch while the least influential factor found was peptone by PlackettBurman method. Peptone amount was kept constant throughout the fermentation. Peptone, which is one of the expensive media components was used at a concentration of 1?g/L, which made the optimum media composition cost effective. A support vector regression-based process model was developed for approximating the non-linear relationship between the fermentation operating variables and the a-amylase yield. Multicanonical Jump Walk Annealing, a stochastic optimization technique is used to obtain optimal operating variables to maximize amylase yield. The maximum amylase activity thus obtained was in good agreement with the experimental values at the optimized levels. The optimum media composition obtained by this method was: yeast extract: 4.53?g/L, starch: 20.246?g/L, K2HPO4: 0.0827%, MgSO4: 0.15%, peptone: 1?g/L. A maximum enzyme activity of 297?U/mL, which was achieved using the above approaches compares well with the activity of reported amylases producing maltooligosaccharides.</p>10aAlkaline a-amylase10aOptimization10aStreptomyces10aSupport vector regression analysis1 aSharma, Trupti, K.1 aBhadane, Vaibhav, A.1 aKumar, Lalitha, S.1 aRele, Meenakshi, V.1 aBhawar, Gajanan1 aRahman, Imran  uhttps://library.ncl.res.in/content/optimization-production-maltooligosaccharides-producing-amylase-alkalophilic-streptomyces-002281nas a2200229   4500008004100000022001400041245013300055210006900188260011000257300001400367490000700381520133800388653002501726653001501751653004201766653001701808100002301825700003001848700002601878700001801904856012901922       2014                        eng d  a0019-518900aAnti-fungal potentials of extracellular metabolites of western ghats isolated streptomyces sp NII 1006 against moulds and yeasts0 aAntifungal potentials of extracellular metabolites of western gh  aDR K S KRISHNAN MARG, PUSA CAMPUS, NEW DELHI 110 012, INDIAbNATL INST SCIENCE COMMUNICATION-NISCAIRcNOV  a1138-11460 v523 a<p>Realization of hazardious effects of chemical fungicides has led to an interest in the usage of biocontrol agents. The present study, therefore, evaluates the biocontrol efficacy of Western Ghats (India) soil bacterial isolates. A potential strain NII 1006 was evaluated for its antagonistic property against a diverse range of moulds and yeasts. The strain was characterized morphologically, biochemically and molecularly, which revealed the isolate belonged to Streptomyces genus. Organic solvent extracts of NII 1006 culture filtrates inhibited the growth of the test pathogens indicating that growth suppression was due to extracellular anti-fungal metabolites present in the culture filtrates. The strain produced extracellular chitinase enzyme in addition to some stable partially purified anti-fungal compounds. Morphological changes such as hyphae degradation into debris and abnormal shapes were observed in test fungi and yeast grown on potato dextrose broth that contained the NII 1006 culture filtrate. The cell free supernatant has a tolerance to wide range of pH, temperature and enzymes such as lipase and protease. The biocontrol potential of NII 1006 strain may be correlated significantly with their ability to produce antibiotics as well as extracellular hydrolytic enzymes particularly chitinolytic enzyme.</p>10aAnti-fungal activity10aBiocontrol10aExtracellular anti-fungal metabolites10aStreptomyces1 aJayamurthy, Himani1 aSajna, Kuttavan, Valappil1 aDastager, Syed, Gulam1 aPandey, Ashok  uhttps://library.ncl.res.in/content/anti-fungal-potentials-extracellular-metabolites-western-ghats-isolated-streptomyces-sp-002290nas a2200253   4500008004100000022001400041245012700055210006900182260011000251300001400361490000700375520131500382653001701697653002301714653002101737653001701758653001501775100002701790700002401817700002001841700002601861700002001887856012901907       2014                        eng d  a0019-518900aDevelopment of bioconjugate from streptomyces tyrosinase and gold nanoparticles for rapid detection of phenol constituents0 aDevelopment of bioconjugate from streptomyces tyrosinase and gol  aDR K S KRISHNAN MARG, PUSA CAMPUS, NEW DELHI 110 012, INDIAbNATL INST SCIENCE COMMUNICATION-NISCAIRcNOV  a1071-10810 v523 a<p>Most of the phenol compounds are toxic and have been considered as hazardous pollutants. Several physicochemical and biological methods are available to detect and monitor the phenol pollutants in water and soil. In the present study, phenol constituents of winery, paper and plastic industrial effluents were successfully detected employing tyrosinase-gold nanoparticles bioconjugate. The synthesis of extracellular tyrosinase and gold nanoparticles was achieved by a single isolate of Streptomyces sp. DBZ-39. Enhanced production (369.41 IU) of tyrosinase was produced in submerged bioprocess employing response surface method with central composite design. Extracellular gold nanoparticles synthesized (12-18 nm) by Streptomyces sp. DBZ-39 were characterized with TEM, EDAX and FTIR analysis. A rapid detection (within 10 min) of phenol constituents from winery effluents was achieved by bioconjugate, when compared to tyrosinases and gold nanoparticles independently. Streptomyces tyrosinase could exhibit relatively a better performance than commercially available mushroom tyrosinase in the detection of phenol constituents. Winery effluent has shown much higher content (0.98 O.D) of phenol constituents than paper and plastic effluents based on the intensity of color and U.V absorption spectra.</p>10aBioconjugate10agold nanoparticles10aPhenol detection10aStreptomyces10aTyrosinase1 aZainab, Mazhari, Bi Bi1 aMadhusudhan, D., N.1 aRaghavendra, H.1 aDastager, Syed, Gulam1 aDayanand, Agsar  uhttps://library.ncl.res.in/content/development-bioconjugate-streptomyces-tyrosinase-and-gold-nanoparticles-rapid-detection-0