01875nas a2200217   4500008004100000022001400041245013000055210006900185260009000254300001000344490000700354520103800361653002201399653001501421653002301436653001601459653001601475100002001491700001701511856012901528       2013                        eng d  a1024-242200aBiotransformation of vinblastine to vincristine by the endophytic fungus fusarium oxysporum isolated from catharanthus roseus0 aBiotransformation of vinblastine to vincristine by the endophyti  aTELEPHONE HOUSE, 69-77 PAUL STREET, LONDON EC2A 4LQ, ENGLANDbINFORMA HEALTHCAREcAPR  a89-930 v313 a<p>A fungal transformation method for the production of the less abundant but more valuable anticancer drug vincristine using the more abundant anticancer drug vinblastine by an endophytic fungus Fusarium oxysporum isolated from the plant Catharanthus roseus is described. Vinblastine dissolved in sterile water when incubated with the fungal mycelial mass was transformed into vincristine at room temperature. The biotransformation process was monitored by periodic sampling of aliquots that were extracted with ethyl acetate. The crude transformed compounds were analysed using TLC where the Rf value of the spot obtained on the 8th day was found to be similar to that of standard vincristine (Rf 0.74). Purification by HPLC using a C 18 symmetry column showed a peak with RT (34.9 min) similar to that of standard vincristine. Further characterization with the help of ESI-MS showed a peak at m/z 825 attributed to the (M + H)(+) ion. This indicated that the fungus Fusarium oxysporum had converted vinblastine to vincristine.</p>10aBiotransformation10aendophytes10afusarium oxysporum10aVinblastine10avincristine1 aKumar, Ashutosh1 aAhmad, Absar  uhttps://library.ncl.res.in/content/biotransformation-vinblastine-vincristine-endophytic-fungus-fusarium-oxysporum-isolated-001841nas a2200265   4500008004100000022001400041245009000055210006900145260007900214300000800293490000700301520090900308653002601217653002501243653002401268653002201292653002501314653002201339653001601361100002001377700002401397700001901421700001601440856011901456       2014                        eng d  a1093-326300aMolecular recognition pattern of cytotoxic alkaloid vinblastine with multiple targets0 aMolecular recognition pattern of cytotoxic alkaloid vinblastine   a360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USAbELSEVIER SCIENCE INCcNOV  a1-90 v543 a<p>Vinblastine (VLB), a cytotoxic alkaloid is used extensively against various cancer types and the crystal structure of its tubulin complex is already known. Multitarget affinity of vinblastine has been investigated and the nature of binding with biological receptors namely, duplex DNA and Human serum albumin (HSA) has been compared to the binding characteristics of its known complex with natural high affinity receptor tubulin using molecular docking and QM-MM calculations. VLB is found to interact with DNA as well as HSA protein, though, with weaker affinity as compared to tubulin. Analysis of various docked complexes revealed that the H-bonds and cation-pi bonds do not have significant contribution to the binding interactions and despite its large size, VLB remains in relaxed conformation and fits in the hydrophobic regions on the receptors. (C) 2014 Elsevier Inc. All rights reserved.</p>10aDrug-DNA interactions10aDrug-protein binding10aHuman serum albumin10aMolecular docking10aMultitarget affinity10aQM-MM calculation10aVinblastine1 aPandya, Prateek1 aAgarwal, Lokesh, Kr1 aGupta, Neelima1 aPal, Sourav  uhttps://library.ncl.res.in/content/molecular-recognition-pattern-cytotoxic-alkaloid-vinblastine-multiple-targets-002729nas a2200253   4500008004100000022001400041245018000055210006900235260000800304300001400312490000700326520183900333653001802172653001002190653001502200653000902215653001602224653001402240100002002254700002602274700002402300700002402324856012702348       2021                        eng d  a0971-589400aZCTs knockdown using antisense LNA GapmeR in specialized photomixotrophic cell suspensions of Catharanthus roseus: Rerouting the flux towards mono and dimeric indole alkaloids0 aZCTs knockdown using antisense LNA GapmeR in specialized photomi  cJUL  a1437-14530 v273 aThe present study was carried out to silence the transcription factor genes ZCT1, ZCT2 and ZCT3 via lipofectamine based antisense LNA GapmeRs transfection into the protoplasts of established photomixotrophic cell suspensions. The photomixotrophic cell suspensions with a threshold of 0.5% sucrose were raised and established using two-tiered CO2 providing flasks kept under high light intensity. The photomixotrophic cell suspensions showed morphologically different thick-walled cells under scanning electron microscopic analysis in comparison to the simple thin-walled parenchymatous control cell suspensions. The LC-MS analysis registered the vindoline production (0.0004 +/- 0.0001 mg/g dry wt.) in photomixotrophic cell suspensions which was found to be absent in control cell suspensions. The protoplasts were isolated from the photomixotrophic cell suspensions and subjected to antisense LNA GapmeRs silencing. Three lines, viz. Z1A, Z2C and Z3G were obtained where complete silencing of ZCT1, ZCT2 and ZCT3 genes, respectively, was observed. The Z3G line was found to show maximum production of vindoline (0.038 +/- 0.001 mg/g dry wt.), catharanthine (0.165 +/- 0.008 mg/g dry wt.) and vinblastine (0.0036 +/- 0.0003 mg/g dry wt.). This was supported by the multifold increment in the gene expression of TDC, SLS, STR, SGD, d4h, dat, CrT16H and Crprx. The present work indicates the master regulation of ZCT3 knockdown among all three ZCTs transcription factors in C. roseus to enhance the terpenoid indole alkaloids production. The successful silencing of transcription repressor genes has been achieved in C. roseus plant system by using photomixotrophic cell cultures through GapmeR based silencing. The present study is a step towards metabolic engineering of the TIAs pathway using protoplast transformation in C. roseus.10aCatharanthine10aLC-MS10aProtoplast10aTIAs10aVinblastine10aVindoline1 aVerma, Priyanka1 aKhan, Shamshad, Ahmad1 aParasharami, Varsha1 aMathur, Ajay, Kumar  uhttps://library.ncl.res.in/content/zcts-knockdown-using-antisense-lna-gapmer-specialized-photomixotrophic-cell-suspensions