02633nas a2200217 4500008004100000245015900041210006900200260000800269300001500277490000600292520177700298653002302075653003902098653002302137653001702160653002302177653003302200100002902233700003002262856012302292 2025 eng d00aComprehensive investigation on statistical approaches and classical strain improvement for penicillin V production by Penicillium rubens BIONCL P45 strain0 aComprehensive investigation on statistical approaches and classi cJAN a1302 -13270 v53 a

Media engineering and strain improvement are critical aspects of microbial biotechnology playing a vital role in enhancing microbial productivity, and ensuring cost-effective bioprocessing. In this investigation, we optimized the various medium components, nutritional condition, and fermentation parameters for the industrial production of phenoxymethylpenicillin or penicillin V (PenV). We have isolated, characterized Penicillium rubens BIONCL P45 strain which initially produced 100 mg/L of PenV. Further, optimization using production medium 4 (PM4) comprising lactose, corn steep solids, sodium sulfate, calcium carbonate, and phenoxy acetic acid lead to a significant increase in production, reaching 430 mg/L. Further improvements through response surface methodology (RSM) predicted a production of 646 mg/L, which was experimentally validated at 685 mg/L. Subsequently, mutagenesis studies using UV (ultraviolet) exposure resulted in the UV-65 mutant, which demonstrated a superior performance, achieving 934 mg/L, surpassing the parental strain. ​These combined strategies lead to a tenfold increase in PenV titer, highlighting their effectiveness in bioprocess development and industrial-scale antibiotic production.

10aBox-Behnken design10aClassical strain improvement (CSI)10aMedia optimization10aPenicillin V10aPenicillium rubens10aResponse surface methodology1 aSawant, Amol, Muralidhar1 aVamkudoth, Koteswara, Rao uhttps://library.ncl.res.in/content/comprehensive-investigation-statistical-approaches-and-classical-strain-improvement