02868nas a2200337   4500008004100000022001400041245018300055210006900238260000900307300001600316490000700332520173300339653002402072653001602096653001202112653001302124653002502137653001802162100002302180700002102203700001402224700001602238700002102254700001902275700002302294700002002317700002302337700002502360700002002385856012502405       2023                        eng d  a1944-824400aAtomically dispersed manganese on graphene nanosheets as biocompatible nanozyme for glutathione detection in liver tissue lysate using microfluidic paper-based analytical devices0 aAtomically dispersed manganese on graphene nanosheets as biocomp  cOCT   a47902-479200 v153 a<p>
	Recently, single atom catalysts (SACs) featuring M-N-x (M = metal) active sites on carbon support have drawn considerable attention due to their promising enzyme-like catalytic properties. However, typical synthesis methods of SACs often involve energy-intensive carbonization processes. Herein, we report a facile one-pot, low-temperature, wet impregnation method to fully utilize M-N-4 sites of manganese phthalocyanine (MnPc) by decorating molecular MnPc over the sheets of graphene nanoplatelets (GNP). The synthesized MnPc@GNP exhibits remarkable peroxidase-mimic catalytic activity toward the oxidation of chromogenic 3,3 `,5,5(')-tetramethylbenzidine (TMB) substrate owing to the efficient utilization of atomically dispersed Mn and the high surface-to-volume ratio of the porous catalyst. A nanozyme-based colorimetric sensing probe is developed to detect important biomarker glutathione (GSH) within only 5 min in solution phase based on the ability of GSH to effectively inhibit the TMB oxidation. The high sensitivity and selectivity of the developed colorimetric assay enable us to quantitatively determine GSH concentration in different biological fluids. This work, for the first time, reports a rapid MnPc@GNP nanozyme-based colorimetric assay in the solid substrate by fabricating microfluidic paper-based analytical devices (mu PADs). GSH is successfully detected on the fabricated mu PADs coated with only 6.0 mu g of nanozyme containing 1.6 nmol of Mn in the linear range of 0.5-10 mu M with a limit of detection of 1.23 mu M. This work also demonstrates the quantitative detection of GSH in mice liver tissue lysate using mu PADs, which paves the way to develop mu PADs for point-of-care testing.</p>
10acolorimetricsensing10aGlutathione10amu PADs10ananozyme10asingle atom catalyst10atissue lysate1 aBaruah, Diksha, J.1 aThakur, Ashutosh1 aRoy, Esha1 aRoy, Kallol1 aBasak, Sumanjita1 aNeog, Dipankar1 aBora, Himangsu, K.1 aKonwar, Rituraj1 aChaturvedi, Vikash1 aShelke, Manjusha, V.1 aDas, Manash, R.  uhttps://library.ncl.res.in/content/atomically-dispersed-manganese-graphene-nanosheets-biocompatible-nanozyme-glutathione02780nas a2200361   4500008004100000022001400041245016100055210006900216260000900285300001600294490000700310520160800317653001501925653001701940653002201957653001301979653001401992653001102006100002102017700002102038700002202059700002102081700002202102700002202124700001902146700002302165700002102188700001802209700002302227700002502250700002002275856012302295       2024                        eng d  a1944-824400aHexagonal boron nitride quantum dots embedded on layer-by-layer films for peroxidase-assisted colorimetric detection of β-galactosidase producing pathogens0 aHexagonal boron nitride quantum dots embedded on layerbylayer fi  cMAY   a26870-268850 v163 a<p>
	Pathogen detection has become a major research area all over the world for water quality surveillance and microbial risk assessment. Therefore, designing simple and sensitive detection kits plays a key role in envisaging and evaluating the risk of disease outbreaks and providing quality healthcare settings. Herein, we have designed a facile and low-cost colorimetric sensing strategy for the selective and sensitive determination of beta-galactosidase producing pathogens. The hexagonal boron nitride quantum dots (h-BN QDs) were established as a nanozyme that showed prominent peroxidase-like activity, which catalyzes 3,3 `,5,5 `-tetramethylbenzidine (TMB) oxidation by H2O2. The h-BN QDs were embedded on a layer-by-layer assembled agarose biopolymer. The beta-galactosidase enzyme partially degrades beta-1,4 glycosidic bonds of agarose polymer, resulting in accessibility of h-BN QDs on the solid surface. This assay can be conveniently conducted and analyzed by monitoring the blue color formation due to TMB oxidation within 30 min. The nanocomposite was stable for more than 90 days and was showing TMB oxidation after incubating it with Escherichia coli (E. coli). The limit of detection was calculated to be 1.8 x 10(6) and 1.5 x 10(6) CFU/mL for E. coli and Klebsiella pneumonia (K. pneumonia), respectively. Furthermore, this novel sensing approach is an attractive platform that was successfully applied to detect E. coli in spiked water samples and other food products with good accuracy, indicating its practical applicability for the detection of pathogens in real samples.</p>
10abiopolymer10acolorimetric10ah-BN quantum dots10ananozyme10apathogens10aSensor1 aMajumdar, Sristi1 aGogoi, Devipriya1 aBoruah, Purna, K.1 aThakur, Ashutosh1 aSarmah, Priyakhee1 aGogoi, Parishmita1 aSarkar, Sanjib1 aPachani, Priyakshi1 aManna, Prasenjit1 aSaikia, Ratul1 aChaturvedi, Vikash1 aShelke, Manjusha, V.1 aDas, Manash, R.  uhttps://library.ncl.res.in/content/hexagonal-boron-nitride-quantum-dots-embedded-layer-layer-films-peroxidase-assisted