02399nas a2200301   4500008004100000245017300041210006900214260000800283300000600291490000700297520134600304653002601650653001101676653002501687653001601712653002801728100001701756700001901773700003301792700002001825700002001845700002301865700001801888700001801906700002201924700002001946856013101966       2023                        eng d00aWhole genome sequencing and comparative genomics of Indian isolates of wheat spot blotch pathogen bipolaris sorokiniana reveals expansion of pathogenicity gene clusters0 aWhole genome sequencing and comparative genomics of Indian isola  cJAN  a10 v123 a<p>
	Spot blotch is a highly destructive disease in wheat caused by the fungal pathogen Bipolaris sorokiniana (teleomorph, Cochliobolus sativus). It is prevalent in warm and humid areas, including Africa, Asia, Latin America, and the USA. In the present study, twelve isolates of B. sorokiniana were collected from wheat fields in three different geographical locations in India. The pathogenicity of seven sporulating isolates was assessed on `DDK 1025', a spot blotch-susceptible wheat variety under greenhouse conditions. The isolate `D2' illustrated the highest virulence, followed by `SI' and `BS52'. These three isolates were sequenced using the Illumina HiSeq1000 platform. The estimated genome sizes of the isolates BS52, D2, and SI were 35.19 MB, 39.32 MB, and 32.76 MB, with GC contents of 48.48%, 50.43%, and 49.42%, respectively. The numbers of pathogenicity genes identified in BS52, D2, and SI isolates were 2015, 2476, and 2018, respectively. Notably, the isolate D2 exhibited a relatively larger genome with expanded arsenals of Biosynthetic Gene Clusters (BGCs), CAZymes, secretome, and pathogenicity genes, which could have contributed to its higher virulence among the tested isolates. This study provides the first comparative genome analysis of the Indian isolates of B. sorokiniana using whole genome sequencing.</p>
10aBipolaris sorokiniana10aCAZyme10acomparative genomics10aspot blotch10awhole genome sequencing1 aYadav, Sagar1 aRaazi, Zarrine1 aShivaraj, Sheelavanta, Matha1 aSomani, Deepika1 aPrashant, Ramya1 aKulkarni, Abhijeet1 aKumar, Rajeev1 aBiradar, Suma1 aDesai, Shreenivas1 aKadoo, Narendra  uhttps://library.ncl.res.in/content/whole-genome-sequencing-and-comparative-genomics-indian-isolates-wheat-spot-blotch-pathogen02657nas a2200241   4500008004100000022001400041245011900055210006900174260000800243300001400251490000700265520179100272653004202063653002502105653002202130653002502152653002602177653001602203100002502219700002002244700002002264856013102284       2025                        eng d  a0032-086200aComprehensive genomic analysis of bipolaris sorokiniana strains: insights into genetic diversity and pathogenicity0 aComprehensive genomic analysis of bipolaris sorokiniana strains   cSEP  a2054-20730 v743 a<p>
	Bipolaris sorokiniana is an important plant-pathogenic fungus that primarily infects cereals, such as wheat and barley, causing diseases such as spot blotch, common root rot and seedling blight. This study presents a comprehensive comparative genomic analysis of nine B. sorokiniana strains based on 16 genomic parameters, including genome completeness, virulence factors, secondary metabolite biosynthesis, effector proteins and CAZymes, to explore their genetic diversity, genome assembly quality and pathogenic potential. Genomic assemblies of the strains LK93 and ND93-1 exhibited higher N50, L50, BUSCO completeness scores and gene annotations, and were designated as high-quality. Similarly, ND90Pr and BS112 demonstrated a rich arsenal of CAZymes and effector proteins, which indicated their greater infection potential. Variability in biosynthetic gene clusters, especially the presence of isocyanide-NRPS and fungal RiPP clusters, highlights the ecological adaptability and metabolic diversity of these strains. The study also revealed distinct protein family distributions and effector protein repertoires, supporting strain-specific pathogenic strategies. Phylogenetic analyses grouped the strains into three clusters, indicating evolutionary divergence and potential ecological specialisation. The findings of the study underscore the importance of high-quality genomic data and propose LK93 and ND93-1 as more reliable reference genomes for B. sorokiniana. Integrating transcriptomics, proteomics and pathogenicity data, along with expanded strain sampling and unified assembly pipelines, can enhance understanding of the molecular basis of pathogenicity and help develop suitable disease management strategies to control this agriculturally important pathogen.</p>
10a<italic>Cochliobolus sativus</italic>10acomparative genomics10aeffector proteins10apathogen variability10aSecondary metabolites10aspot blotch1 aShukla, Anand, Kumar1 aSahoo, Rosaleen1 aKadoo, Narendra  uhttps://library.ncl.res.in/content/comprehensive-genomic-analysis-bipolaris-sorokiniana-strains-insights-genetic-diversity-and