02765nas a2200229   4500008004100000022001400041245014100055210006900196260000900265300001100274490000800285520195600293653002202249653001502271653002002286653001702306653002202323100002302345700001802368700001902386856013002405       2021                        eng d  a0048-969700aMetagenomic analysis of a mega-city river network reveals microbial compositional heterogeneity among urban and peri-urban river stretch0 aMetagenomic analysis of a megacity river network reveals microbi  cAUG   a1469600 v7833 a<p>The rivers in the megacities face a constant inflow of extremely polluted wastewaters from various sources, and their influence on the connected peri-urban river is still poorly understood. The riverine system in Pune consists of Rivers Mula, Ramnadi, Pawana, Mutha, and Mula-Mutha, traversing through the urban settlements of Pune before joining River Bhima in the peri-urban region. We used MinION-based metagenomic sequencing to generate a comprehensive understanding of the microbial diversity differ-ences between the urban and peri-urban zones, which has not been explored at the meta scale until date. The taxonomic analysis revealed significant enrichment of pollution indicators microbial taxa (Welsch's t-test, p &lt; 0.05, Benjamini-Hochberg FDR test) such as Bacteriodetes, Firmicutes, Spirochaetes, Synergistetes, Euryarcheota in the urban waters as compared to peri-urban waters. Further, the peri-urban waters showed a significantly higher prevalence of ammonium oxidising archaeal groups such as Nitrososphaeraceae (Student's t-test p-value &lt;0.05 with FDR correction), thereby probably suggesting the influence of agricultural runoffs. Besides, the microbial community diversity assessment also indicated the significant dissimilarity in the microbial community of urban and peri-urban waters. Overall, the analysis predicted 295 virulence genes mapping to 38 different path-ogenic bacteria in the riverine system. Moreover, the higher genome coverage (at least 60%) for priority patho-gens such as Pseudomonas, Klebsiella, Acinetobacter, Escherichia, Aeromonas in the sediment metagenome consolidates their dominance in this riverine system. To conclude, our investigation showed that the unre-strained anthropogenic and related activities could potentially contribute to the overall dismal conditions and in-fluence the connected riverine stretches on the outskirts of the city . (c) 2021 Elsevier B.V. All rights reserved.</p>10aIndustrialisation10aPeri-urban10aRiverine system10aUrbanisation10aVirulence factors1 aYadav, Rakeshkumar1 aRajput, Vinay1 aDharne, Mahesh  uhttps://library.ncl.res.in/content/metagenomic-analysis-mega-city-river-network-reveals-microbial-compositional-heterogeneity