02652nas a2200277 4500008004100000022001400041245013600055210006900191260000800260300001200268490000700280520172500287653001902012653001802031653001302049653002602062653001602088653001902104100001902123700002002142700002002162700002002182700002302202700001702225856013202242 2020 eng d a1535-389300aComparative proteomics unravels the differences in salt stress response of own-rooted and 110R-grafted thompson seedless grapevines0 aComparative proteomics unravels the differences in salt stress r cFEB a583-5990 v193 a

Thompson Seedless, a commonly grown table grape variety, is sensitive to salinity when grown on its own roots, and therefore, it is frequently grafted onto salinity-tolerant wild grapevine rootstocks. Rising soil salinity is a growing concern in irrigated agricultural systems. The accumulation of salts near the root zone severely hampers plant growth, leading to a decrease in the productive lifespan of grapevine and causing heavy yield losses to the farmer. In the present study, we investigated the differences in response to salinity between own-rooted Thompson Seedless (TSOR) and 110R-grafted Thompson Seedless (TS110R) grapevines, wherein 110R is reported to be a salt-tolerant rootstock. The grapevines were subjected to salt stress by treating them with a 150 mM NaCl solution. The stress-induced changes in protein abundance were investigated using a label-free shotgun proteomics approach at three time points viz. 6 h, 48 h, and 7 days of salt treatment. A total of 2793 proteins were identified, of which 246 were differentially abundant at various time-points in TSOR and TS110R vines. The abundance of proteins involved in several biological processes such as photosynthesis, amino acid metabolism, translation, chlorophyll biosynthesis, and generation of precursor metabolites was significantly affected by salt stress in both the vines but at different stages of stress. The results revealed that TSOR vines responded fervently to salt stress, while TS110R vines adopted a preventive approach. The findings of this study add to the knowledge of salinity response in woody and grafted plants and hence open the scope for further studies on salt stress-specific differences induced by grafting.

10a110R rootstock10agene ontology10agrafting10alabel-free proteomics10asalt stress10aVitis vinifera1 aPatil, Sucheta1 aShinde, Manisha1 aPrashant, Ramya1 aKadoo, Narendra1 aUpadhyay, Anuradha1 aGupta, Vidya uhttps://library.ncl.res.in/content/comparative-proteomics-unravels-differences-salt-stress-response-own-rooted-and-110r-grafted02888nas a2200253 4500008004100000022001400041245011400055210006900169260000800238300001400246490000700260520204600267653001602313653002002329653001902349653003102368653000802399653001602407653002202423653002102445100002202466700001702488856012902505 2024 eng d a0006-308800a Positive role of polyunsaturated fatty acids on sustainable crop production against salt stress: an overview0 aPositive role of polyunsaturated fatty acids on sustainable crop cJUN a1599-16100 v793 a

Abiotic stresses have become more severe and capricious due to global warming and varying climatic conditions, with increased temperature reducing the yield of important agricultural crops due to high evapotranspiration, resulting in the increased amount of soil salinization in arid and semi-arid areas, which has become a significant threat that restricts agricultural practices and leads to the overexploitation of cultivation land. One of the crucial environmental elements limiting plant development and yield is salinity. The special effects of salt stress on the superiority of numerous crops have yet to be discovered. Under salinity, plants tend to activate multiple physiological and biochemical mechanisms to overcome the stress by altering their morphology, photosynthesis, water relations, and biochemical adaptations, such as the antioxidative metabolism response and trigger polyunsaturated fatty acids (PUFAs), which act as a biomarker for salinity stress. With the help of PUFAs, which have become popular as all-purpose defenders, decorative techniques have been created to prevent the consequences of saline. The most prevalent PUFAs in plants are those with 18 carbons, specifically 18:1 (oleic), 18:2 (linoleic), and 18:3 (alpha-linolenic) acids which operate as glycerolipids, a source of energy and carbon in triacylglycerol, precursors of numerous bioactive chemicals, stores of extracellular barrier components, and intrinsic antioxidants, modulating cellular membranes and enhancing crop quality and yield. However, limited information about PUFAs and their roles in enhancing crop stress tolerance is available. Therefore, producers and breeders must understand salinity's influence on crop composition to enhance fatty acids under salinity conditions. However, brief work has been reported; this review will help comprehend the role of fatty acids in salinity for food security through the genetic engineering of synthetic genes encoding fatty acids to improve crop stress tolerance and grain quality.

10aCuticle wax10aMembrane lipids10aphotosynthesis10aPolyunsaturated fatty acid10aROS10asalt stress10aSensing mechanism10astress tolerance1 aSrivastava, Smita1 aYadav, Sagar uhttps://library.ncl.res.in/content/positive-role-polyunsaturated-fatty-acids-sustainable-crop-production-against-salt-stress02558nas a2200253 4500008004100000022001400041245009000055210006900145260000800214300001200222490000700234520173800241653001901979653001301998653001802011653002302029653001602052653002602068100001602094700002502110700002702135700002402162856011802186 2025 eng d a0971-781100aHarnessing microRNAs and their targets for salt stress tolerance in Pandanus odorifer0 aHarnessing microRNAs and their targets for salt stress tolerance cSEP a714-7300 v343 a

Salinity poses a formidable challenge to agriculture, detrimentally impacting plant growth and the yield of several crops globally. Pandanus odorifer (Forssk.) Kuntze is a highly salt-stress resilient plant that grows naturally along the coastal regions of Asia, Southeast Asia, and Polynesia. It is frequently found as a frontier species along the sandy shores receiving constant salt sprays. MicroRNAs (miRNAs) play essential regulatory roles in growth, development, and combating various biotic and abiotic stresses. Thus, characterizing miRNAs and their targets is critical to understanding their roles in salt stress tolerance. Through a rigorous analysis based on homology, secondary structure and signatures of other miRNA genes, we identified 52 conserved nonredundant miRNAs from the P. odorifer transcriptome data. These miRNAs were diverse and belonged to 23 miRNA phylogenetic families. Several miRNAs, including pod-MIR156a, pod-MIR164d, and pod-MIR535d, demonstrated distinct expression patterns in response to salt stress. Their target genes exhibited opposing expression trends, corroborating the intricate miRNA-target relationship. Delving deeper into their influence, we discovered that these miRNAs mainly targeted transcription factors that are known for their pivotal roles in stress responses. We also detected four miRNA-star (miRNA*) sequences (the complementary strands of functional mature miRNAs), which supports the authenticity of the identified miRNAs. This study unveils the intricate regulatory mechanism through which miRNAs may reinforce salt stress tolerance in P. odorifer, providing a promising avenue for enhancing crop resilience to various stresses through miRNA-based strategies.

10aAbiotic stress10amicroRNA10amiRNA targets10aSalinity tolerance10asalt stress10aTranscription factors1 aRashmi, Deo1 aBarvkar, Vitthal, T.1 aNadaf, Altafhusain, B.1 aKadoo, Narendra, Y. uhttps://library.ncl.res.in/content/harnessing-micrornas-and-their-targets-salt-stress-tolerance-pandanus-odorifer