02593nas a2200253   4500008004100000022001400041245010600055210006900161260000900230300001200239490000800251520168600259653001501945653002701960653002101987653003402008100001802042700003202060700002302092700002602115700002602141700004502167856012702212       2020                        eng d  a0141-813000aStructural, molecular, functional and immunological characterization of Wuchereria bancrofti-galectin0 aStructural molecular functional and immunological characterizati  cMAY   a206-2170 v1503 a<p>Galectins regulate growth and differentiation of immune cells and inflammation through their carbohydrate-binding function in humans, while also play a role in parasite survival. This study focused on the galectin of lymphatic filarial parasite Wuchereria bancrofti (Wb-Gal). The multiple sequence alignment with other galectins showed that the Wb-Gal belonged to galactoside binding lectin family, particularly tandem repeat type galectin-9. A homology model of Wb-Gal was developed in the I-TASser server using high similarity 3D structures with a quality score of 89.5. Molecular docking and dynamics studies revealed that the CCRD and NCRD of Wb-Gal bind with galactose and lactose. Further, Wb-Gal was cloned into the pET28 vector, expressed in E. coli Rosetta strain and purified by affinity chromatography. In the hemagglutination assays, the rWb-Gal bound to lactose, galactose, and glucose. Indirect Enzyme-Linked Immunosorbent Assay (ELISA) using different clinical filarial sera showed that the IgG and IgM response was against Wb-Gal x very high in all filarial clinical groups, whereas the IgA and IgG2 response was minimum to negligible. There was an enhanced response of IgG1 and IgG4 antibodies in Microfilaremics (MF) cases compared to Chronic Pathology (CP) and Endemic Normal (EN) individuals. Interestingly, the IgE response was comparatively higher in EN than MF and CP. These studies show that Wb-Gal is a member of the lectin family of proteins binding to different carbohydrates and may have an important role in the pathophysiology of filarial infection which needs to be investigated in greater detail. (C) 2020 Elsevier B.V. All rights reserved.</p>
10aFilariasis10aHemagglutination assay10aImmunomodulation10aWuchereria bancrofti galectin1 aYasin, Nazeer1 aLaxmanappa, Hoti, Sugerappa1 aMuddapur, Uday, M.1 aCheruvathur, Jennifer1 aPrakash, S., M. Udaya1 aThulasiram, Hirekodathakallu, Venkataram  uhttps://library.ncl.res.in/content/structural-molecular-functional-and-immunological-characterization-wuchereria-bancrofti02765nas a2200229   4500008004100000022001400041245012900055210006900184260000800253300000800261490000800269520187300277653005302150653002102203653003202224653003602256653003402292653003802326100001902364700002102383856013102404       2024                        eng d  a1617-461500aDifferential cellular communication in tumor immune microenvironment during early and advanced stages of lung adenocarcinoma0 aDifferential cellular communication in tumor immune microenviron  cDEC  a1000 v2993 a<p>
	Heterogeneous behavior of each cell type and their cross-talks in tumor immune microenvironment (TIME) refers to tumor immunological heterogeneity that emerges during tumor progression and represents formidable challenges for effective anti-tumor immune response and promotes drug resistance. To comprehensively elucidate the heterogeneous behavior of individual cell types and their interactions across different stages of tumor development at system level, a computational framework was devised that integrates cell specific data from single-cell RNASeq into networks illustrating interactions among signaling and metabolic response genes within and between cells in TIME. This study identified stage specific novel markers which remodel the cross-talks, thereby facilitating immune stimulation. Particularly, multicellular knockout of metabolic gene APOE (Apolipoprotein E in mast cell, myeloid cell and fibroblast) combined with signaling gene CAV1 (Caveolin1 in endothelial and epithelial cells) resulted in the activation of T-cell mediated signaling pathways. Additionally, this knockout also initiated intervention of cytotoxic gene regulations during tumor immune cell interactions at the early stage of Lung Adenocarcinoma (LUAD). Furthermore, a unique interaction motif from multiple cells emerged significant in regulating the overall immune response at the advanced stage of LUAD. Most significantly, FCER1G (Fc Fragment of IgE Receptor Ig) was identified as the common regulator in activating the anti-tumor immune response at both stages. Predicted markers exhibited significant association with patient overall survival in patient specific dataset. This study uncovers the significance of signaling and metabolic interplay within TIME and discovers important targets to enhance anti-tumor immune response at each stage of tumor development.</p>
10aEarly and advanced stages of Lung Adenocarcinoma10aImmunomodulation10aMulticellular communication10aSignaling-metabolic cross-talks10aTumor immune microenvironment10aTumor immunological heterogeneity1 aShukla, Mudita1 aSarkar, Ram, Rup  uhttps://library.ncl.res.in/content/differential-cellular-communication-tumor-immune-microenvironment-during-early-and-advanced