01525nas a2200253 4500008004100000022001400041245007200055210006900127260008700196300001200283490000700295520067800302653002500980653001701005653001801022653001601040100002101056700002401077700001901101700002101120700001601141700001801157856009601175 2005 eng d a0011-389100aAssessing the role of FecB mutation in productivity of Indian sheep0 aAssessing the role of FecB mutation in productivity of Indian sh aC V RAMAN AVENUE, PO BOX 8005, BANGALORE 560 080, INDIAbCURRENT SCIENCE ASSNcSEP a887-8900 v893 a

DNA samples from four Indian sheep breeds, viz. Garole, Deccani, Bannur and Madras Red were screened by PCR-RFLP to determine the presence of FecB mutation in these breeds. The Garole was the only breed, which carried the FecB mutation. The FecB mutation was introgressed from Garole sheep into Deccani sheep and Bannur sheep, and the performance of the crossbred sheep is being monitored in subsequent generations. Approximately half of the first backcross ewes (progeny of FecB heterozygote F1 rams) was found to carry one copy of FecB mutation, as expected. The FecB PCR-RFLP test was found to be fast, accurate and useful as a tool for making breeding decisions.

10aFecB (Booroola) gene10aGarole sheep10aPCR-RFLP test10aprolificacy1 aPardeshi, V., C.1 aSainani, Mohini, N.1 aMaddox, J., F.1 aGhalsasi, P., M.1 aNimbkar, C.1 aGupta, V., S. uhttps://library.ncl.res.in/content/assessing-role-fecb-mutation-productivity-indian-sheep-001793nas a2200229 4500008004100000022001400041245006400055210006400119260006100183300001200244490000800256520104000264100001701304700002501321700002601346700001701372700002001389700002001409700002101429700001801450856009501468 2005 eng d a0040-575200aMolecular mapping of stem and leaf rust resistance in wheat0 aMolecular mapping of stem and leaf rust resistance in wheat a233 SPRING STREET, NEW YORK, NY 10013 USAbSPRINGERcSEP a846-8500 v1113 a

Stem rust caused by Puccinia graminis f. sp. tritici Eriks and Henn and leaf rust caused by Puccinia triticina Rob. ex Desm. are major constraints to wheat production worldwide. In the present study, F-4-derived SSD population, developed from a cross between Australian cultivars `Schomburgk' and `Yarralinka', was used to identify molecular markers linked to rust resistance genes Lr3a and Sr22. A total of 1,330 RAPD and 100 ISSR primers and 33 SSR primer pairs selected on the basis of chromosomal locations of these genes were used. The ISSR marker UBC 840(540) was found to be linked with Lr3a in repulsion at a distance of 6.0 cM. Markers cfa2019 and cfa2123 flanked Sr22 at a distance of 5.9 cM (distal) and 6.0 cM (proximal), respectively. The use of these markers in combination would predict the presence or absence of Sr22 in breeding populations. A previously identified PCR-based diagnostic marker STS638 linked to Lr20 was validated in this population. This marker showed a recombination value of 7.1 cM with Lr20.

1 aKhan, R., R.1 aBariana, Harbans, S.1 aDholakia, Bhushan, B.1 aNaik, S., V.1 aLagu, Meena, D.1 aRathjen, A., J.1 aBhavani, Sridhar1 aGupta, V., S. uhttps://library.ncl.res.in/content/molecular-mapping-stem-and-leaf-rust-resistance-wheat-001885nas a2200277 4500008004100000022001400041245014100055210006900196260008700265300001400352490000700366520086300373653003001236653002201266653001901288653001701307100001701324700002201341700002101363700002001384700002101404700001901425700001901444700001801463856012601481 2006 eng d a0011-389100aGenetic diversity analysis in Gaultheria fragrantissima wall. (Ericaceae) from the two biodiversity hotspots in India using ISSR markers0 aGenetic diversity analysis in Gaultheria fragrantissima wall Eri aC V RAMAN AVENUE, PO BOX 8005, BANGALORE 560 080, INDIAbCURRENT SCIENCE ASSNcDEC a1634-16400 v913 a

Our study involves Inter Simple Sequence Repeat markers to analyse genetic diversity of an undershrub, Gaultheria fragrantissima Wall. (Ericaceae) from two plant diversity hotspots in India, namely Western Ghats and Northeastern Himalayas. The plants from these two regions show no morphological differences but the total heterozygosity (H-T = 0.505) is high. Furthermore, the average heterozygosity of G. fragrantissima at WG (H-s = 0.433) is higher than that at NE region (H-s, = 0.231). Within population variance is higher (15.56 %) than among population variance (8.31 %) as seen in outcrossing plant species. Mantel's test shows a strong positive correlation between the genetic and geographic distances. The plants within WG show high gene flow, which may be enough to prevent genetic drift, however, the gene flow in NE population is very low.

10aGaultheria fragrantissima10aGenetic diversity10aheterozygosity10aISSR markers1 aApte, G., S.1 aBahulikar, R., A.1 aKulkarni, R., S.1 aLagu, Meena, D.1 aKulkarni, B., G.1 aSuresh, H., S.1 aRao, P., S. N.1 aGupta, V., S. uhttps://library.ncl.res.in/content/genetic-diversity-analysis-gaultheria-fragrantissima-wall-ericaceae-two-biodiversity-002291nas a2200301 4500008004100000022001400041245012400055210006900179260008900248300001200337490000700349520127500356653001601631653001601647653000801663653000801671653001801679100001801697700001201715700002601727700002001753700001501773700001801788700001601806700001901822700001801841856013001859 2008 eng d a0733-521000aMolecular genetic mapping of quantitative trait loci associated with loaf volume in hexaploid wheat (Triticum aestivum)0 aMolecular genetic mapping of quantitative trait loci associated  a24-28 OVAL RD, LONDON NW1 7DX, ENGLANDbACADEMIC PRESS LTD ELSEVIER SCIENCE LTDcMAY a587-5980 v473 a

Major efforts in wheat research are being made to improve the yield and quality of wheat. Loaf volume (Lv) is the main quality parameter deciding the bread making potential of wheat. To genetically dissect quantitative trait loci (QTLs) for Lv, a Recombinant Inbred Line (RIL) population (F-8) was developed from a cross between two Indian wheat varieties ``H 1977'' and ``HD 2329''. A total of 914 SSR and 100 ISSR primers were used for molecular analysis and the genetic map comprising 19 chromosomes was constructed with 202 SSR markers and 2 HMW glutenin subunit loci: Glu-B1 and Glu-B1. The phenotypic data were collected from six environments including three different agro-climatic zones for 2 consecutive years. Dissection of Lv through AMMI model revealed significant G x E variance for the trait. QTL analysis was performed using composite interval mapping. A total of 30 QTLs for Lv were detected and significant QTLs were identified on 6B and 6D chromosomes; 1B, 1D, 2A, 3A, 513 and 5D also contributed genetically to Lv. Association between 6B and 6D QTLs and variable expression of gliadins on group 6 chromosomes were discussed. QTLs detected in this study were compared with other QTL analysis in wheat. (C) 2007 Elsevier Ltd. All rights reserved.

10abreadmaking10aloaf volume10aQTL10aSSR10awheat quality1 aElangovan, M.1 aRai, R.1 aDholakia, Bhushan, B.1 aLagu, Meena, D.1 aTiwari, R.1 aGupta, R., K.1 aRao, V., S.1 aRoeder, M., S.1 aGupta, V., S. uhttps://library.ncl.res.in/content/molecular-genetic-mapping-quantitative-trait-loci-associated-loaf-volume-hexaploid-wheat-001707nas a2200253 4500008004100000022001400041245005900055210005900114260007400173300001200247490000700259520089800266653002301164653001801187653002001205653002201225653001901247100002101266700001601287700002401303700001801327700001801345856009001363 2009 eng d a1380-374300aMolecular mapping of wilt resistance genes in chickpea0 aMolecular mapping of wilt resistance genes in chickpea aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcSEP a177-1830 v243 a

Fusarium wilt is a widespread and serious chickpea disease caused by the soil-borne fungus Fusarium oxysporum f.sp. ciceri (Foc). We evaluated an F(9) recombinant inbred line population of chickpea for resistance to three Foc races (1, 2 and 3) in pot culture experiments and identified flanking and tightly linked DNA markers for the resistance genes. The simple sequence repeat markers H3A12 and TA110 flanked the Foc1 locus at 3.9 and 2.1 cM, respectively, while Foc2 was mapped 0.2 cM from TA96 and 2.7 cM from H3A12. The H1B06y and TA194 markers flanked the Foc3 locus at 0.2 and 0.7 cM, respectively. These markers were also validated using 16 diverse chickpea genotypes. Identification of tightly linked flanking markers for wilt resistance genes will be useful for their exploitation in breeding programs and to understand the mechanism of resistance and evolution of the genes.

10afusarium oxysporum10aFusarium wilt10aGenetic mapping10aMarker validation10aPathogen races1 aGowda, S., J. M.1 aRadhika, P.1 aKadoo, Narendra, Y.1 aMhase, L., B.1 aGupta, V., S. uhttps://library.ncl.res.in/content/molecular-mapping-wilt-resistance-genes-chickpea-000709nas a2200169 4500008004100000022001400041245009600055210006900151260010200220300001400322490000700336100001600343700002000359700001800379700001900397856012300416 2010 eng d a0011-389100aAssessment of Pongamia pinnata (L.) - a biodiesel producing tree species using ISSR markers0 aAssessment of Pongamia pinnata L a biodiesel producing tree spec aC V RAMAN AVENUE, SADASHIVANAGAR, P B \#8005, BANGALORE 560 080, INDIAbINDIAN ACAD SCIENCEScNOV a1327-13290 v991 aSujatha, K.1 aRajwade, A., V.1 aGupta, V., S.1 aHazra, Sulekha uhttps://library.ncl.res.in/content/assessment-pongamia-pinnata-l-biodiesel-producing-tree-species-using-issr-markers-001648nas a2200217 4500008004100000022001400041245015200055210006900207260008000276300001000356490000600366520080400372653002101176653001201197653001801209100001801227700002601245700002001271700001801291856012101309 2010 eng d a1835-269300aDifferential accumulation of gliadin proteins in wheat grain of RILs grown at two different agroclimatic conditions and their effect on loaf volume0 aDifferential accumulation of gliadin proteins in wheat grain of  a8 91-93 MCKENZIE ST, LISMORE, NSW 2480, AUSTRALIAbSOUTHERN CROSS PUBLcJAN a63-670 v43 a

The end use of wheat depends on the quality and quantity of protein accumulated in the grain during the grain filling stage. Gliadin families of proteins are proved to affect the bread making quality (BMQ) by affecting the rheology and functionality of dough. The gliadin accumulation was analysed using Indian genotypes grown in two diverse agro climatic zones. About 16 RILs along with their parents HI977 and HD2329 revealed significant difference in accumulation of all gliadin fractions. High accumulation of alpha and beta gliadin with low level of gamma gliadin was observed for Kota as compared to Pune location. However, this significant difference in gliadin accumulation due to environment, did not affect the loaf volume performance of the RILs at statistically significant level.

10aloaf volume (Lv)10aRP-HPLC10aWheat protein1 aElangovan, M.1 aDholakia, Bhushan, B.1 aGaikwad, S., M.1 aGupta, V., S. uhttps://library.ncl.res.in/content/differential-accumulation-gliadin-proteins-wheat-grain-rils-grown-two-different-001928nas a2200241 4500008004100000022001400041245010600055210006900161260007400230300001200304490000800316520107400324653000901398653002201407653001501429653001301444100001601457700002401473700002401497700002001521700001801541856012701559 2010 eng d a0016-670700aUnderstanding the genetic structure of symplocos laurina wall. populations using nuclear gene markers0 aUnderstanding the genetic structure of symplocos laurina wall po aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcFEB a197-2100 v1383 a

To characterize the genetic diversity of present populations of Symplocos laurina, which grow in the montane forests in India, we analyzed the DNA sequences of a nuclear gene. Using the 881 bp sequence of cytosolic Glyceraldehyde-3-phosphate dehydrogenase gene, we detected 24 haplotypes among 195 individuals sampled from 14 populations. Two dominant haplotypes were distributed over the entire range of this species in India and several private haplotypes were found. Low genetic diversity within population, high differentiation, number of population specific haplotypes and deviation from neutral evolution characterized the present populations of S. laurina. An analysis of molecular variance indicated the presence of geographic structure within the haplotype distribution. The occurrence of S. laurina preglaciation in India is the most parsimonious explanation for the current geographic structure observed. The populations are presumably ancient and might have spread across its extant distribution range in India through a recent range expansion event.

10aGapC10aGenetic diversity10aGlaciation10aRefugium1 aBanu, Sofia1 aBhagwat, Rasika, M.1 aKadoo, Narendra, Y.1 aLagu, Meena, D.1 aGupta, V., S. uhttps://library.ncl.res.in/content/understanding-genetic-structure-symplocos-laurina-wall-populations-using-nuclear-gene-002165nas a2200265 4500008004100000022001400041245006900055210006900124260008000193300000900273490000700282520128000289653002001569653001701589653003401606653002301640653001701663100002101680700001601701700001801717700002201735700001801757700002401775856010001799 2011 eng d a1234-198300aMapping of QTLs governing agronomic and yield traits in chickpea0 aMapping of QTLs governing agronomic and yield traits in chickpea aTIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANYbSPRINGER HEIDELBERGcFEB a9-210 v523 a

Chickpea is one of the most important leguminous cool season food crops, cultivated prevalently in South Asia and Middle East. The main objective of this study was to identify quantitative trait loci (QTLs) associated with seven agronomic and yield traits in two recombinant inbred line populations of chickpea derived from the crosses JG62xVijay (JV population) and VijayxICC4958 (VI population) from at least three environments. Single locus QTL analysis involved composite interval mapping (CIM) for individual traits and multiple-trait composite interval mapping (MCIM) for correlated traits to detect pleiotropic QTLs. Two-locus analysis was conducted to identify the main effect QTLs (M-QTLs), epistatic QTLs (E-QTLs) and QTL x environment interactions. Through CIM analysis, a total of 106 significant QTLs (41 in JV and 65 in VI populations) were identified for the seven traits, of which one QTL each for plant height and days to maturity was common in both the populations. Six pleiotropic QTLs that were consistent over the environments were also identified. LG2 in JV and LG1a in VI contained at least one QTL for each trait. Hence, concentrating on these LGs in molecular breeding programs is most likely to bring simultaneous improvement in these traits.

10aCicer arietinum10aQTL analysis10aQTL x environment interaction10aTwo-locus analysis10aYield traits1 aGowda, S., J. M.1 aRadhika, P.1 aMhase, L., B.1 aJamadagni, B., M.1 aGupta, V., S.1 aKadoo, Narendra, Y. uhttps://library.ncl.res.in/content/mapping-qtls-governing-agronomic-and-yield-traits-chickpea-002233nas a2200313 4500008004100000022001400041245007500055210006900130260007400199300001200273490000700285520122400292653002301516653001401539653003001553653002701583653001001610100002601620700002001646700001601666700001701682700001501699700002101714700002001735700002201755700001801777700001801795856010601813 2013 eng d a1380-374300aMolecular mapping of leaf rust resistance gene Lr15 in hexaploid wheat0 aMolecular mapping of leaf rust resistance gene Lr15 in hexaploid aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcMAR a743-7470 v313 a

Leaf rust is a widespread and commonly occurring rust disease of wheat. Genetic resistance is the most economical method of reducing losses due to leaf rust. Lr15 has been shown to be present on wheat chromosome 2D and is reported to be a seedling resistance gene. However, tightly linked markers associated with Lr15 have not been reported to date. To identify molecular markers linked to Lr15, an F-2 mapping population of Thatcher x Thatcher-Lr15 was generated. Available wheat simple sequence repeat markers were utilized in parental screening and polymorphic markers were used to analyze the entire population of 221 plants. Phenotypic evaluations of the F-2-derived F-3 progenies with Puccinia triticina Eriks. pathotype 162A (93R15) confirmed the monogenic inheritance of Lr15. The linkage group representing chromosome 2DS was constructed at LOD 4.0 which revealed the closest flanking markers Xgwm4562 and Xgwm102 at a distance of 3.1 and 9.3 cM, respectively. Furthermore, utilization of these flanking markers in combination has successfully identified wheat lines with or without Lr15. These markers could potentially be useful in gene pyramiding with other genes to enhance rust resistance in wheat.

10aDisease resistance10aLeaf rust10aMarker-assisted selection10aSimple sequence repeat10aWheat1 aDholakia, Bhushan, B.1 aRajwade, A., V.1 aHosmani, P.1 aKhan, R., R.1 aChavan, S.1 aReddy, D., M. R.1 aLagu, Meena, D.1 aBansal, Urmil, K.1 aSaini, R., G.1 aGupta, V., S. uhttps://library.ncl.res.in/content/molecular-mapping-leaf-rust-resistance-gene-lr15-hexaploid-wheat-002219nas a2200217 4500008004100000022001400041245014700055210006900202260011500271300001200386490000700398520132900405653002501734653001901759100001701778700001601795700001801811700002401829700001801853856013001871 2015 eng d a0006-313400aExpression of stable reference genes and SPINDLY gene in response to gibberellic acid application at different stages of grapevine development0 aExpression of stable reference genes and SPINDLY gene in respons aROZVOJOVA 263, PRAHA 6, CZ-165 02, CZECH REPUBLICbACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANYcSEP a436-4440 v593 a

Gibberellic acid (GA(3)) is widely used at different stages of berry development, and to understand the molecular mechanism of its action requires identification of stable reference genes. We sprayed grapevine (Vitis vinifera L.) cv. Thompson Seedless with GA(3) at rachis stage for rachis elongation, at flower cluster stage for flower thinning, and at 3 - 4 mm berry stage for berry elongation. Tissue samples were collected at different time points after GA(3) application. The expression of 10 candidate reference genes was analyzed using 4 different algorithms to assess their suitability for real time-PCR data normalization. Based on the overall ranking, PP2A, Sutra, and SAND were identified as the most stably expressed genes across all samples. With regard to different stages, tubulin, EF1 alpha, and UBC were the most stable genes during rachis elongation; PP2A, SAND, and Sutra were the most suitable at the flower cluster and berry stages. The expression of GA signaling gene SPINDLY (VvSpy) was analyzed to validate the stable reference genes. After the GA(3) application, the expression of VvSpy was reduced at the rachis stage but did not change at the flower cluster and berry stages. The expression profile of VvSpy was comparable when two or three reference genes were used for data normalization.

10adevelopmental stages10aVitis vinifera1 aUpadhyay, A.1 aJogaiah, S.1 aMaske, S., R.1 aKadoo, Narendra, Y.1 aGupta, V., S. uhttps://library.ncl.res.in/content/expression-stable-reference-genes-and-spindly-gene-response-gibberellic-acid-application-002108nas a2200169 4500008004100000245005000041210004700091260003800138300001200176520157700188100002101765700001501786700002001801700001801821700001801839856008101857 2016 eng d00aBalancing omega-6: Omega-3 ratios in oilseeds0 aBalancing omega6 Omega3 ratios in oilseeds bSpringer International Publishing a203-2203 aFats and oils are the essential constituents of human diet, and nearly 80 % of these are obtained from plants. The predominant fatty acids present in plant oils are saturated and unsaturated compounds with straight aliphatic chains of carbon atoms and a single carboxyl group. Depending on the position of the first double bond from the methyl end in the fatty acyl chain, the mono- and polyunsaturated fatty acids can be denoted as -9, -6, or -3. Excess consumption of -6 fatty acids has greatly and unfavorably increased the -6: -3 ratio up to 25:1, which is associated with prevalence of many negative health effects, including cardiovascular diseases, cancer, osteoporosis, and inflammatory and autoimmune diseases. Because the -3 fatty acids (FAs) are the precursors for synthesis of anti-inflammatory eicosanoids, balancing the -6: -3 ratio is vital. The easiest approach to achieve this would be consumption of oils rich in -3 FAs, such as linseed oil. Alternatively, the fatty acid biosynthetic pathway in plants producing high -6 FAs can be altered by biotechnological means, so that they produce higher proportion of -3 FAs. This chapter describes the current knowledge of the fatty acid biosynthesis pathway in plants, including the genes involved, their temporal and spatial expression patterns, and various fluxes that they drive. The choice of oilseeds, genes, and promoters for modulating the fatty acid biosynthesis flux from -6 to -3 fatty acids is also dealt with. The potential hurdles in achieving these and possible solutions have also been described.1 aChirmade, T., P.1 aSanghi, S.1 aRajwade, A, .V.1 aGupta, V., S.1 aKadoo, N., Y. uhttps://library.ncl.res.in/content/balancing-omega-6-omega-3-ratios-oilseeds02592nas a2200217 4500008004100000245015900041210006900200260000800269300001200277490000700289520180400296100001902100700001602119700001802135700001802153700001802171700001902189700001702208700001802225856013102243 2016 eng d00aCapsicum annuum proteinase inhibitor ingestion negatively impacts the growth of sorghum pest Chilo partellus and promotes differential protease expression0 aCapsicum annuum proteinase inhibitor ingestion negatively impact cDEC a302-3090 v783 aBackground Chilo partellus is an important insect pest infesting sorghum and maize. The larvae internalize in the stem, rendering difficulties in pest management. We investigated the effects of Capsicum annuum proteinase inhibitors (CanPIs) on C. partellus larvae by in-vitro and in-vivo experiments. Methods Recombinant CanPI-7 (with four-Inhibitory Repeat Domains, IRDs), -22 (two-IRDs) and insect proteinase activities were estimated by proteinase assays, dot blot assays and in gel activity assays. Feeding bioassays of lab reared C. partellus with CanPI-7 and -22 were performed. C. partellus proteinase gene expression was done by RT-PCR. In-silico structure prediction of proteinases and CanPI IRDs was carried out, their validation and molecular docking was done for estimating the interaction strength. Results Larval proteinases of C. partellus showed higher activity at alkaline pH and expressed few proteinase isoforms. Both CanPIs showed strong inhibition of C. partellus larval proteinases. Feeding bioassays of C. partellus with CanPIs revealed a dose dependent retardation of larval growth, reduction of pupal mass and fecundity, while larval and pupal periods increased significantly. Ingestion of CanPIs resulted in differential up-regulation of C. partellus proteinase isoforms, which were sensitive to CanPI-7 but were insensitive to CanPI-22. In-silico interaction studies indicated the strong interaction of IRD-9 (of CanPI-22) with Chilo proteinases tested. Conclusions Of the two PIs tested, CanPI-7 prevents induction of inhibitor insensitive proteinases in C. partellus so it can be explored for developing C. partellus tolerance in sorghum. General significance Ingestion of CanPIs, effectively retards C. partellus growth; while differentially regulating the proteinases.1 aJadhav, A., R.1 aWar, A., R.1 aNikam, A., N.1 aAdhav, A., S.1 aGupta, V., S.1 aSharma, H., C.1 aGiri, A., P.1 aTamhane, V.A. uhttps://library.ncl.res.in/content/capsicum-annuum-proteinase-inhibitor-ingestion-negatively-impacts-growth-sorghum-pest-chilo01863nas a2200193 4500008004100000245009000041210006900131260000800200300001400208490000600222520121600228100002001444700001901464700002001483700001801503700001801521700001701539856011301556 2016 eng d00aData of in vitro synthesized dsRNAs on growth and development of helicoverpa armigera0 aData of in vitro synthesized dsRNAs on growth and development of cJUN a1602-16050 v73 aThe data presented in this article is related to the research article "RNAi of selected candidate genes interrupts growth and development of Helicoverpa armigera" (Chikate et al., 2016) [1]. RNA interference (RNAi) is emerging as a potent insect pest control strategy over current methods and their resistance by pest. In this study we tested 15 different in vitro synthesized dsRNAs for gene silencing in Helicoverpa armigera. These dsRNAs were specific against H. armigera enzymes/proteins such as proteases like trypsins (HaTry2, 3, 4 and 6), chymotrypsin (HaChy4) and cysteine proteases such as cathepsin (HaCATHL); glutathione S-transferases (HaGST1a, 6 and 8); esterases (HaAce4, HaJHE); catalase (HaCAT); super-oxide-dismutase (HaCu/ZnSOD); fatty acid binding protein (HaFabp) and chitin deacetylase (HaCda5b). These dsRNAs were fed to second instar larvae at an optimized dose (60 μg/day) for 3 days separately. Effects of dsRNA feeding were observed in terms of larval mass gain, percentage mortality and phenotypic abnormalities in later developmental stages of H. armigera. These findings might provide potential new candidates for designing sequence-specific dsRNA as pesticide in crop protection. 1 aChikate, Y., R.1 aDawkar, V., V.1 aBarbole, R., S.1 aTilak, P., V.1 aGupta, V., S.1 aGiri, A., P. uhttps://library.ncl.res.in/content/data-vitro-synthesized-dsrnas-growth-and-development-helicoverpa-armigera01333nas a2200193 4500008004100000245016700041210006900208260000800277300001200285490000600297520059900303100002200902700002000924700001600944700001900960700001700979700001800996856012501014 2016 eng d00aData on changes in the fatty acid composition during fruit development and ripening of three mango cultivars (Alphonso, Pairi and Kent) varying in lactone content0 aData on changes in the fatty acid composition during fruit devel cDEC a480-4910 v93 aData in this article presents fatty acid composition of three mango cultivars; Alphonso, Pairi and Kent through fruit development and ripening. Change in the ω-6 and ω-3 fatty acids level during mango fruit development and ripening is depicted. Also, data on aroma volatile ‘lactones’ composition from pulp and skin tissues of these cultivars at their ripe stage, respectively is provided. Statistical data is also shown, which correlates modulation in lactone content with that of fatty acid composition and content during fruit development and ripening in all the three mango cultivars.1 aDeshpande, A., B.1 aChidley, H., G.1 aOak, P., S.1 aPujari, K., H.1 aGiri, A., P.1 aGupta, V., S. uhttps://library.ncl.res.in/content/data-changes-fatty-acid-composition-during-fruit-development-and-ripening-three-mango02887nas a2200169 4500008004100000245008700041210006900128260000800197520226400205100002002469700001802489700002502507700002502532700002202557700003002579856010802609 2018 eng d00aPatho-physiological evaluation of Duranta erecta for the treatment of urolithiasis0 aPathophysiological evaluation of Duranta erecta for the treatmen cMAY3 a

Background: Urolithiasis is the third common disorder of the urinary system affecting 10-15% of the general population. In recent years, search for new antilithiatic drugs from natural sources has assumed greater importance. Objectives: This study was performed to investigate the anti-urolithiatic activity of methanolic extract of Duranta erecta leaves by in vitro and in vivo analysis. Materials and methods: The study was designed to determine presence of phytochemicals in D. erecta, its yield in percentage, antioxidant activity against 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and anti-microbial property against few bacteria. In vitro analysis was carried out study anti-urolithiatic property of D. erecta by nucleation assay and synthetic urine assay for inhibition of calcium oxalate and calcium oxalate monohydrate crystals formation. An in vivo experiment was performed on Wistar rats for confirmation of anti-urolithiatic property of D. erecta in animal model. Results: D. erecta has the presence of primary and secondary metabolites like glycoside, saponins, sterols, flavonoids, phenols, tannins, alkaloids, carbohydrates and proteins. Methanolic extract of D. erecta gave a very good yield (60%). D. erecta proved its antioxidant potential by 93.51% inhibition of DPPH radical at a concentration of 1000 μg/mL where ascorbic showed 94.71% of DPPH radical at the same concentration. In vitro tests like nucleation assay and synthetic urine assay showed that D. erecta inhibits formation of calcium oxalate and calcium oxalate monohydrate crystals. It also showed the anti-microbial property by formation of zone of inhibition against few bacteria. An in vivo experiment on Wistar rat animal model confirmed the anti-urolithiatic property of D. erecta L. leaves extract. Conclusions: Based on the results, we reported that D. erecta may treat calcium oxalate crystal deposition in the kidney by preventing hyperoxaluria-induced peroxidative damage to the renal tubular membrane surface (lipid peroxidation). It has anti-microbial potential so it may also inhibit the secondary bacterial infection in kidney. Based on the data, it can be concluded that this herb can be used as a potential anti-urolithiasis agent for kidney stone removal.

1 aAgawane, S., B.1 aGupta, V., S.1 aKulkarni, Mahesh, J.1 aBhattacharya, A., K.1 aKoratkar, Santosh1 aRao, Vankudoth, Koteswara uhttps://library.ncl.res.in/content/patho-physiological-evaluation-duranta-erecta-treatment-urolithiasis01191nas a2200193 4500008004100000245009100041210006900132260000800201300001200209490000700221520053700228100001600765700002200781700001900803700002400822700001700846700001800863856011600881 2019 eng d00aData on metabolic profiling of spongy tissue disorder in Mangifera indica cv. Alphonso0 aData on metabolic profiling of spongy tissue disorder in Mangife cFEB a145-1570 v223 aData in this article presents aroma volatiles and fatty acids composition of mesocarp specific malady namely spongy tissue disorder in Mangifera indica cv. Alphonso. Quantitative changes in various aroma volatile compound classes as well as saturated and unsaturated fatty acids in spongy tissue vis-à-vis healthy mesocarp have been analyzed throughout the development of the disorder. Statistical data analysis correlates the dynamic changes in the aroma volatiles composition to that of the modulation in the fatty acids profile.1 aOak, P., S.1 aDeshpande, A., B.1 aPujari, K., H.1 aPrabhudesai, S., S.1 aGiri, A., P.1 aGupta, V., S. uhttps://library.ncl.res.in/content/data-metabolic-profiling-spongy-tissue-disorder-mangifera-indica-cv-alphonso02309nas a2200253 4500008004100000022001400041245015900055210006900214260000800283300001200291490000700303520144900310653001701759653001101776653001401787653001401801653001401815653001301829100001901842700001801861700002101879700002401900856013101924 2022 eng d a0972-593800aPharmaceutical standardization and physicochemical characterization of traditional ayurvedic mineral drug red ochre roasted in cow's ghee (Shuddha Gairik)0 aPharmaceutical standardization and physicochemical characterizat cAPR a303-3160 v213 a

Rasashastra is a pharmaceutics branch of Ayurveda describing scientific methods to convert metals and minerals into bio-compatible formulations used individually or plant admixed to enhance their bioavailability and efficacy. In the present study, red ochre (Gairik) powder was processed in cow's ghee by textual method of roasting. The steps of preparation and changes in the properties therein were documented and validated in triplicate batches to develop a monograph. Ayurvedic and basic tests were performed to determine the properties of Shuddha Gairik. The physical characterization included Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD), Fourier Transform Infra-red (FT-IR), Dynamic Light Scattering (DLS) and Thermo-gravimetric Analysis (TGA). Elemental composition was estimated by titration and gravimetric analysis while heavy metal limits were assessed using Inductively Coupled Plasma Optical Emission Spectrophotometry (ICPOES). This study depicted that crude red ochre, containing Kaolinite with high iron percentage, on roasting in cow's ghee led to the formation of fatty acids adsorbed red ochre particles. The developed monograph will be a guideline to the Ayurvedic industry for precise formulation of Shuddha Gairik. This will help researchers for better understanding the importance of Ayurvedic methods of pharmaceutical preparations and carry out their mechanistic studies in various diseases.

10aFerric oxide10aGairik10aKaolinite10aMonograph10aRed ochre10aRoasting1 aChavan, S., B.1 aGupta, V., S.1 aDeshmukh, V., V.1 aSardeshmukh, S., P. uhttps://library.ncl.res.in/content/pharmaceutical-standardization-and-physicochemical-characterization-traditional-ayurvedic-0