02970nas a2200301   4500008004100000022001400041245013000055210006900185260005700254300001400311490000800325520189300333653003402226653001102260653002302271653001502294653001302309653003002322653002902352100002602381700002302407700002402430700002002454700002102474700002002495700002402515856012902539       2015                        eng d  a0171-521600aRole of tumor cell surface lysosome-associated membrane protein-1 (LAMP1) and its associated carbohydrates in lung metastasis0 aRole of tumor cell surface lysosomeassociated membrane protein1   a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcSEP  a1563-15740 v1413 a<p>Expression of lysosome-associated membrane protein-1 (LAMP1) on the surface correlates with metastatic potential of B16 melanoma cells. Downregulation of their expression in high metastatic (B16F10) cells reduced their surface expression and metastatic potential. Present investigations explore if overexpression of LAMP1 on the surface of low metastatic (B16F1) cells augment their metastatic ability, and if so, how? B16F1 cells were transduced with lentiviral vector carrying mutant-LAMP1 (Y386A) (mutLAMP1). Surface expression of LAMP1 and carbohydrates was analyzed by flow cytometry, immunofluorescence and/or immunoprecipitation and Western blotting. Cell spreading and motility were assessed on components of extracellular matrix (ECM) (fibronectin) and basement membrane (BM) (matrigel), and galectin-3-coated coverslips/plates. Metastatic potential was assessed using experimental metastasis assay. Pre-incubation with anti-LAMP1 antibodies significantly reduced lung metastasis of B16F10 cells. Overexpression of mutLAMP1 significantly increased its surface expression on B16F1 cells, resulting in increased cellular spreading and motility on fibronectin and matrigel. LAMP1 is the major carrier of poly-N-acetyllactosamine (polyLacNAc) on B16F10 cells. However, significantly higher expression of mutLAMP1 had no effect on galectin-3 binding on cell surface or on spreading or motility of cells on galectin-3-coated coverslips/plates. These cells also failed to show any gain in metastatic ability. This could be because LAMP1 from these cells carried significantly lower levels of polyLacNAc in comparison with B16F10 cells. PolyLacNAc on B16F10 cells and galectin-3 on lungs are the major participants in melanoma metastasis. Although surface LAMP1 promotes interactions with organ ECM and BM, carbohydrates on LAMP1 play a decisive role in dictating lung metastasis.</p>10a6 branched N-oligosaccharides10abeta 110aCell surface LAMP110aGalectin-310aMotility10aOrgan-specific metastasis10aPoly-N-acetyllactosamine1 aAgarwal, Akhil, Kumar1 aSrinivasan, Nithya1 aGodbole, Rashmi, K.1 aMore, Shyam, K.1 aBudnar, Srikanth1 aGude, Rajiv, P.1 aKalraiya, Rajiv, D.  uhttps://library.ncl.res.in/content/role-tumor-cell-surface-lysosome-associated-membrane-protein-1-lamp1-and-its-associated-0