02043nas a2200169   4500008004100000245009100041210006900132260000800201300001400209490000600223520141200229100002101641700003001662700002501692700003301717856012301750       2015                        eng d00aFactors influencing L-asparaginase production by three thermophilic caprophilous fungi0 aFactors influencing Lasparaginase production by three thermophil  cSEP  a1242-12480 v53 a<p>Influence of medium, pH and temperature on L-asparaginase production by Thielavia terrestris, Malbranchea cinnamomea and Scytalidium thermophilum in submerged fermentation process was investigated. Maximum Lasparaginase production was recorded in the glucose medium supplemented with 0.5% L-asparagine monohydrate on 8th day of incubation. Czapek-dox medium was the next proffered medium for both biomass and L-asparaginase production by the fungi under investigation. Among different pH tried, pH 7.0 was optimum for L-asparaginase production, while enzyme production was marginal in medium with 6.0-8.0. Temperature of 45°C and 50°C were optimum for T. terrestris for growth of M. cinnamomea and S. thermophilum respectively. Maximum Lasparaginase activity of all three fungi was recorded at 45°C with varying extent was recorded. Analysis of variance of the data revealed significant differences (p-0.05) among the different culture media, pH and temperature on the production of L-asparaginase. A significant and positive correlation coefficient (R) was also observed between the growth and L-asparaginase activity of T. terrestris (0.570), M. cinnamomea (0.947) and S. thermophilum (0.948). Conclusion the culture medium and factors like pH, incubation temperature and incubation period play an important role in the L-asparaginase production by all the three thermophilic caprophilous fungi.</p>1 aShanthipriya, A.1 aRao, Vankudoth, Koteswara1 aSivadevuni, Girisham1 aReddy, Solipuram, Madhusudha  uhttps://library.ncl.res.in/content/factors-influencing-l-asparaginase-production-three-thermophilic-caprophilous-fungi