02732nas a2200241 4500008004100000245010700041210006900148260000800217300001400225490000800239520187700247100001802124700001902142700002002161700001802181700002302199700003302222700002702255700002202282700003702304700002102341856012802362 2016 eng d00aNotch1-MAPK signaling axis regulates CD133+ cancer stem cell-mediated melanoma growth and angiogenesis0 aNotch1MAPK signaling axis regulates CD133 cancer stem cellmediat cDEC a2462-24740 v1363 a

Functional characterization and understanding of the intricate signaling mechanisms in stem-like cells is crucial for the development of effective therapies in melanoma. We have studied whether melanoma cells are phenotypically distinct and hierarchically organized according to their tumorigenic nature. We report that melanoma-specific CD133(+) cancer stem cells exhibit increased tumor-initiating potential, tumor-endothelial cell interaction, and lung metastasis. These cells are able to transdifferentiate into an endothelial-like phenotype when cultured under endothelial differentiation-promoting conditions. Mechanistically, Notch1 upregulates mitogen-activated protein kinase activation through CD133, which ultimately controls vascular endothelial growth factor and matrix metalloproteinase expression in CD133(+) stem cells leading to melanoma growth, angiogenesis, and lung metastasis. Blockade or genetic ablation of Notch1 and mitogen-activated protein kinase pathways abolishes melanoma cell migration and angiogenesis. Chromatin immunoprecipitation and reporter assays revealed that Notch1 intracellular domain regulates CD133 expression at the transcriptional level. Andrographolide inhibits Notch1 intracellular domain expression, Notch1 intracellular domaindependent CD133-mediated mitogen-activated protein kinase and activator protein-1 activation, and epithelial to mesenchymal-specific gene expression, ultimately attenuating melanoma growth and lung metastasis. Human malignant melanoma specimen analyses revealed a strong correlation between Notch1 intracellular domain, CD133, and p-p38 mitogen-activated protein kinase expression and malignant melanoma progression. Thus, targeting Notch1 and its regulated signaling network may have potential therapeutic implications for the management of cancer stem cell-mediated melanoma progression.

1 aKumar, Dhiraj1 aKumar, Santosh1 aGorain, Mahadeo1 aTomar, Deepti1 aPatil, Harshal, S.1 aRadharani, Nalukurthi, N. V.1 aKumar, Totakura, V. S.1 aPatil, Tushar, V.1 aThulasiram, Hirekodathakallu, V.1 aKundu, Gopal, C. uhttps://library.ncl.res.in/content/notch1-mapk-signaling-axis-regulates-cd133-cancer-stem-cell-mediated-melanoma-growth-and02898nas a2200313 4500008004100000022001400041245015200055210006900207260000800276300000700284490000700291520187700298653001702175653001402192653001802206653001402224653001502238653000902253100001802262700001902280700002002299700001902319700002202338700002002360700001702380700003702397700002102434856012902455 2018 eng d a1471-240700aEpoxyazadiradione suppresses breast tumor growth through mitochondrial depolarization and caspase-dependent apoptosis by targeting PI3K/Akt pathway0 aEpoxyazadiradione suppresses breast tumor growth through mitocho cJAN a520 v183 a

Background: Breast cancer is one of the most commonly diagnosed invasive cancers among women around the world. Among several subtypes, triple negative breast cancer (TNBC) is highly aggressive and chemoresistant. Treatment of TNBC patients has been challenging due to heterogeneity and devoid of well-defined molecular targets. Thus, identification of novel effective and selective agents against TNBC is essential. Methods: We used epoxyazadiradione to assess the cell viability, mitochondrial potential, ROS level, cell migration, apoptosis and protein expression in cell culture models of TNBC MDA-MB-231 and ER+MCF-7 breast cancer cells. The molecular mechanism was examined in two different type of breast cancer cells in response to epoxyazadiradione. We have also analyzed the effect of epoxyazadiradione on breast tumor growth using in vivo mice model. Results: In this study, we for the first time investigated that out of 10 major limonoids isolated from Azadirachta indica, epoxyazadiradione exhibits most potent anti-cancer activity in both TNBC and ER+breast cancer cells. Epoxyazadiradione induces apoptosis and inhibits PI3K/Akt-mediated mitochondrial potential, cell viability, migration and angiogenesis. It also inhibits the expression of pro-angiogenic and pro-metastatic genes such as Cox2, OPN, VEGF and MMP-9 in these cells. Furthermore, epoxyazadiradione attenuates PI3K/Akt- mediated AP-1 activation. Our in vivo data revealed that epoxyazadiradione suppresses breast tumor growth and angiogenesis in orthotopic NOD/SCID mice model. Conclusion: Our findings demonstrate that epoxyazadiradione inhibits PI3K/Akt-dependent mitochondrial depolarisation, induces apoptosis and attenuates cell migration, angiogenesis and breast tumor growth suggesting that this compound may act as a potent therapeutic agent for the management of breast cancer.

10aAngiogenesis10aApoptosis10aBreast cancer10aLimonoids10aMetastasis10aPI3K1 aKumar, Dhiraj1 aHaldar, Saikat1 aGorain, Mahadeo1 aKumar, Santosh1 aMulani, Fayaj, A.1 aYadav, Amit, S.1 aMiele, Lucio1 aThulasiram, Hirekodathakallu, V.1 aKundu, Gopal, C. uhttps://library.ncl.res.in/content/epoxyazadiradione-suppresses-breast-tumor-growth-through-mitochondrial-depolarization-and02121nas a2200205 4500008004100000245012900041210006900170260000800239300001600247490000600263520136900269100002101638700002001659700002001679700002401699700002101723700002201744700002701766856012201793 2019 eng d00aGraphene oxide supported liposomes as red emissive theranostics for phototriggered tissue visualization and tumor regression0 aGraphene oxide supported liposomes as red emissive theranostics  cJUL a3312–33200 v23 a

Selective tissue visualization and localized tumor regression without affecting the surrounding healthy tissues are critical concerns in cancer nanomedicine. Importantly, the complete wrapping of a flimsy matrix like liposome by multifunctional graphene oxide is an interesting engineering idea for nanomedicine design. Moreover, designing a safe and biodegradable nanohybrid with significant theranostic ability is a current need for targeted combined therapies. Here, we report a comprehensive result of in vivo tumor diagnosis and phototriggered tumor regression using a biodegradable red emissive nanotheranostic system, viz., graphene oxide flakes fortified liposome (GOF-Lipo), functionalized with folic acid (FA): GOF-Lipo-FA. Graphene oxide support enhances the stability of drug-loaded liposomes in an extracellular environment that prevents the premature release of loaded anticancer drug from the liposomal cavity. Promising outcomes of tumor regression (∼300 to 25 mm3) from organized cellular and animal studies are demonstrated in this work. These studies reveal superior biocompatibility, deep intracellular localization, 4T1 breast tumor diagnosis, and long time tumor binding ability of an injected emissive nanohybrid. Overall, a single dose of designed multifunctional systems demonstrates the best tumor regression.

1 aPrasad, Rajendra1 aYadav, Amit, S.1 aGorain, Mahadeo1 aChauhan, Deepak, S.1 aKundu, Gopal, C.1 aSrivastava, Rohit1 aSelvaraj, Kaliaperumal uhttps://library.ncl.res.in/content/graphene-oxide-supported-liposomes-red-emissive-theranostics-phototriggered-tissue02118nas a2200181 4500008004100000245012900041210006900170260000800239300001600247490000600263520143700269100001801706700002001724700002001744700001701764700002501781856013001806 2019 eng d00aVivo wound healing performance of halloysite clay and gentamicin-incorporated cellulose ether-PVA electrospun nanofiber mats0 aVivo wound healing performance of halloysite clay and gentamicin cSEP a4324–43340 v23 a

Wound healing is a dynamic and complex process that requires a suitable environment to enhance the rapid healing process. In this context, fabrications of nanofibrous materials with antibiotic and antibacterial properties are becoming extremely important. In this present work, we report on the fabrication and characterization of electro-spun cellulose ether-PVA nanofiber mats loaded with halloysite clay (HNT) and gentamicin sulfate (GS) for faster wound healing applications. The morphology of nanofiber mats was examined by SEM and TEM. The average diameter of the nanofiber mats were in the range of 325 ± 30 nm. The physicochemical characterizations were done by FT-IR and XRD, which reveal the presence of HNT and GS into the nanofibers. The incorporation of halloysite gave good mechanical strength to the nanofiber mats. Swelling studies indicated the hydrophilicity of the mats. In vitro studies revealed that HNTs are nontoxic to L929 fibroblast cells and also promote cell growth and proliferation. The antibacterial property of HNT was also studied. The slow release of GS from the nanofiber mats was observed for a period of 18 days. The in vivo wound healing studies on the wistar rats for 21 days revealed the wound healing faster within 2 weeks by the incorporation of HNT and GS into the nanofiber mats and hence these nanofiber mats show great potential in acute and chronic wound healing applications.

1 aWali, Ashwini1 aGorain, Mahadeo1 aInamdar, Satish1 aKundu, Gopal1 aBadiger, Manohar, V. uhttps://library.ncl.res.in/content/vivo-wound-healing-performance-halloysite-clay-and-gentamicin-incorporated-cellulose-ether00933nas a2200253 4500008004100000022001400041245012600055210006900181260000800250300001400258490000700272653002400279653001800303653002600321100002600347700002300373700002200396700002300418700002000441700003800461700002100499700002800520856013100548 2024 eng d a2373-987800aEngineered PLGA core-lipid shell hybrid nanocarriers improve the efficacy and safety of irinotecan to combat colon cancer0 aEngineered PLGA corelipid shell hybrid nanocarriers improve the  cSEP a6661-66760 v1010aBULK-POLYMERIZATION10aDrug-delivery10aLIPOSOMAL DOXORUBICIN1 aGiram, Prabhanjan, S.1 aNimma, Ramakrishna1 aBulbule, Anuradha1 aYadav, Amit, Singh1 aGorain, Mahadeo1 aRadharani, Nalukurthi, Naga Venka1 aKundu, Gopal, C.1 aGarnaik, Baijayantimala uhttps://library.ncl.res.in/content/engineered-plga-core-lipid-shell-hybrid-nanocarriers-improve-efficacy-and-safety-irinotecan02552nas a2200229 4500008004100000022001400041245015300055210006900208260000900277300001400286490000600300520168500306100002601991700002302017700002202040700002302062700002002085700003802105700002102143700002802164856013002192 2024 eng d a2470-134300aPoly(d,l-lactide-co-glycolide) surface-anchored biotin-loaded irinotecan nanoparticles for active targeting of colon cancer0 aPolyscdscsclsclactideicoiglycolide surfaceanchored biotinloaded  cJAN  a3807-38260 v93 a

A poly-(d,l-lactide-co-glycolide) (PLGA) copolymer was synthesized using the ring-opening polymerization of d,l-lactide and glycolide monomers in the presence of zinc proline complex in bulk through the green route and was well characterized using attenuated total reflectance-Fourier transform infrared, H-1 and C-13 nuclear magnetic resonance, gel permeation chromatography, differential scanning calorimetry, X-ray diffraction, matrix-assisted laser desorption/ionization time-of-flight, etc. Furthermore, PLGA-conjugated biotin (PLGA-B) was synthesized using the synthesized PLGA and was employed to fabricate nanoparticles for irinotecan (Ir) delivery. These nanoparticles (PLGA-NP-Ir and PLGA-B-NP-Ir) were tested for physicochemical and biological characteristics. PLGA-B-NP-Ir exhibited a stronger cellular uptake and anticancer activity as compared to PLGA-NP-Ir in CT-26 cancer cells (log p < 0.05). The accumulation and retention of fluorescence-labeled nanoparticles were observed to be better in CT-26-inoculated solid tumors in Balb/c mice. The PLGA-B-NP-Ir-treated group inhibited tumor growth significantly more (log p < 0.001) than the untreated control, PLGA-NP-Ir, and Ir-treated groups. Furthermore, no body weight loss, hematological, and blood biochemical tests demonstrated the nanocarriers' nontoxic nature. This work presents the use of safe PLGA and the demonstration of a proof-of-concept of biotin surface attached PLGA nanoparticle-mediated active targeted Ir administration to combat colon cancer. To treat colon cancer, PLGA-B-NP-Ir performed better due to specific active tumor targeting and greater cellular uptake due to biotin.

1 aGiram, Prabhanjan, S.1 aNimma, Ramakrishna1 aBulbule, Anuradha1 aYadav, Amit, Singh1 aGorain, Mahadeo1 aRadharani, Nalukurthi, Naga Venka1 aKundu, Gopal, C.1 aGarnaik, Baijayantimala uhttps://library.ncl.res.in/content/polydl-lactide-co-glycolide-surface-anchored-biotin-loaded-irinotecan-nanoparticles-active13257nas a2200169 45000080041000002450145000412100069001862600008002553000011002634900006002745201260500280100001812885700002012903700001712923700002112940856012612961 2024 eng d00aSilver nanoparticles in electrospun ethyl hydroxy ethyl cellulose-PVA Nanofiber: synthesis, characterization and wound dressing applications0 aSilver nanoparticles in electrospun ethyl hydroxy ethyl cellulos cJUN a1004770 v73 a

Electrospinning is a simple, cost-effective technique and a reproducible process for both synthetic and natural polymers. It is found to be an attractive tool for various applications in biomedical engineering, filtration, protective clothing, catalysis reactions and sensors. Non-ionic cellulose ethers namely Ethyl Hydroxy Ethyl Cellulose (EHEC) is an important polysaccharide which is non-toxic, biocompatible and biodegradable and finds applications as thickening/rheology control agents in paints, cosmetics, detergents, oil recovery and also in the biomedical area. Poly (vinyl alcohol) (PVA) is a semi-crystalline hydrophilic polymer which is highly biocompatible and non-toxic having good thermal and mechanical properties and also high water solubility. Silver shows powerful antimicrobial activity and is non-toxic. It has been used to cure severe burns and chronic ulcers for many decades and is extensively used as an additive in various fields like prostheses, burn treatment, catheters, vascular grafts, dental resin components, ion exchange fibers, stainless steel materials, human skin and coating of medical devices. In the present work, EHEC/PVA nanofibers embedded with silver nanoparticles (AgNPs) are studied for their antibacterial activity using both gram positive and gram negative bacteria. The nanofibers show increase in tensile properties with the addition of silver nano-particles. The AgNPs embedded EHEC/PVA nanofiber mats having 0.5% AgNPs were found to enhance the wound healing with no scar formation in wistar rats. The biological in-vitro and in-vivo studies support the potential of EHEC based nanofibers as excellent bio-materials for the treatment of severe burns and wounds. To the best of our knowledge, this is the first report on the electrospinning of EHEC/PVA nanofibers for wound healing applications.

1 aWali, Ashwini1 aGorain, Mahadeo1 aKundu, Gopal1 aBadiger, Manohar uhttps://library.ncl.res.in/content/silver-nanoparticles-electrospun-ethyl-hydroxy-ethyl-cellulose-pva-nanofiber-synthesis