01929nas a2200217   4500008004100000245013800041210006900179260000800248490000600256520112400262100002301386700002101409700002001430700002301450700001901473700002101492700002401513700002001537700002801557856012601585       2016                        eng d00aProtein-dependent membrane interaction of a partially disordered protein complex with oleic acid : Implications for cancer lipidomics0 aProteindependent membrane interaction of a partially disordered   cOCT0 v63 a<p>Bovine α-lactalbumin (BLA) forms cytotoxic complexes with oleic acid (OA) that perturbs tumor cell membranes, but molecular determinants of these membrane-interactions remain poorly understood. Here, we aim to obtain molecular insights into the interaction of BLA/BLA-OA complex with model membranes. We characterized the folding state of BLA-OA complex using tryptophan fluorescence and resolved residue-specific interactions of BLA with OA using molecular dynamics simulation. We integrated membrane-binding data using a voltage-sensitive probe and molecular dynamics (MD) to demonstrate the preferential interaction of the BLA-OA complex with negatively charged membranes. We identified amino acid residues of BLA and BLA-OA complex as determinants of these membrane interactions using MD, functionally corroborated by uptake of the corresponding α-LA peptides across tumor cell membranes. The results suggest that the α-LA component of these cytotoxic complexes confers specificity for tumor cell membranes through protein interactions that are maintained even in the lipid complex, in the presence of OA.</p>1 aChaudhuri, Arunima1 aPrasanna, Xavier1 aAgiru, Priyanka1 aChakraborty, Hirak1 aRydstrom, Anna1 aHo, James, C. S.1 aSvanborg, Catharina1 aSengupta, Durba1 aChattopadhyay, Amitabha  uhttps://library.ncl.res.in/content/protein-dependent-membrane-interaction-partially-disordered-protein-complex-oleic-acid