01595nas a2200253   4500008004100000022001400041245008600055210006900141260008100210300001200291490000700303520073500310653001701045653001101062653002001073653002401093653002401117100001501141700001801156700001501174700001501189700001701204856012001221       2006                        eng d  a0950-542300aRapid molecular identification of freshly squeezed and reconstituted orange juice0 aRapid molecular identification of freshly squeezed and reconstit  a9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLANDbBLACKWELL PUBLISHINGcJUN  a646-6510 v413 a<p>This work presents a rapid and accurate molecular approach in the identification of freshly squeezed and reconstituted orange juice. Primers were designed based on the 18S and internal transcribed spacer (ITS) region. The findings indicated that self-made freshly squeezed orange juice heated to 80 degrees C for 30 s caused damage to DNA integrity, thus failing in amplification by primer 18S-ITSe, which gives a polymerase chain reaction product of 2081 bp, whereas primer 18S-ITSf, fragment length 2421 bp, could only be seen in heating conditions less stringent than 70 degrees C, 15 s. This new DNA integrity-dependent method has potential applications in identifying adulteration of freshly or processed orange juice.</p>10aadulteration10acitrus10aDNA degradation10amolecular detection10areconstituted juice1 aNg, C., C.1 aChang, C., C.1 aWu, I., C.1 aKotwal, S.1 aShyu, Y., T.  uhttps://library.ncl.res.in/content/rapid-molecular-identification-freshly-squeezed-and-reconstituted-orange-juice-0