02044nas a2200265   4500008004100000022001400041245011200055210006900167260010600236300001400342490000700356520101800363653003301381653002101414653003101435653002101466100002301487700002201510700001901532700001801551700002201569700003701591700001801628856013201646       2016                        eng d  a0960-894X00aAzadirachta indica triterpenoids promote osteoblast differentiation and mineralization in vitro and in vivo0 aAzadirachta indica triterpenoids promote osteoblast differentiat  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcAUG  a3719-37240 v263 a<p>Terpenoids were isolated using chromatographic purification through solvent purification technique and identified as Azadirone (1), Epoxyazadiradione (2) Azadiradione (3) Gedunin (4) Nimbin (5) Salannin (6) Azadirachtin A (7) and Azadirachtin B (8) from Azadirachta indica. Out of eight compounds, only three compounds had osteogenic activity and enhanced osteoblast proliferation, differentiation and mineralization in osteoblast cells. Active compounds stimulated osteogenic genes ALP, RunX-2 and OCN expressions in vitro, but Azadirachtin A had a maximum ability to stimulate osteoblast differentiation and mineralization compared to other two active compounds. For in vivo study, Azadirachtin A injected subcutaneously in pups, which enhanced osteogenic gene expressions and promoted bone formation rate significantly. Here, we conclude that active compounds of Azadirachta indica have osteogenic activity and Azadirachtin A has a beneficial effects on bone. (C) 2016 Elsevier Ltd. All rights reserved.</p>10aALP and mineralization assay10aBalb/c mice pups10aCalvarial osteoblast cells10aGene expressions1 aKushwaha, Priyanka1 aKhedgikar, Vikram1 aHaldar, Saikat1 aGautam, Jyoti1 aMulani, Fayaj, A.1 aThulasiram, Hirekodathakallu, V.1 aTrivedi, Ritu  uhttps://library.ncl.res.in/content/azadirachta-indica-triterpenoids-promote-osteoblast-differentiation-and-mineralization-vitro02740nas a2200181   4500008004100000022001400041245012200055210006900177260000900246300001600255490000600271520207900277100002202356700002702378700001902405700000502424856012902429       2021                        eng d  a2470-134300aAccurate identification of bioactive meliaceae limonoids by UHPLC-MS/ms based structure-fragment relationships (SFRs)0 aAccurate identification of bioactive meliaceae limonoids by UHPL  cOCT   a26454-264760 v63 aY Limonoids are bioactive plant specialized metabolites found in the Meliaceae family. The basic limonoids, i.e., azadiradione, epoxyazadiradione, and gedunin have been exploited for various bioactivities and therefore are the potential drug leads for tomorrow. However, their low abundance, structural similarity, and lack of adequate mass fragmentation data have hampered their accurate identification and quantification from various sources. In the present study, basic limonoids such as azadirone, azadiradione, epoxyazadiradione, and gedunin isolated from Neem were utilized for the synthesis of their derivatives and isotopologs. A total of 30 one compounds were used in this study among which five were isolated, two were biotransformed, and 24 were synthesized. Among the synthesized compounds nine are novel compounds including six deuterated analogs/isotopologs which are (1,3-H-2)-1,2dihydro-3 beta-hydroxyazadiradione (9), (1,3,16-H-2)-1,2-dihydro-3 beta-16 beta-dihydroxyazadiradione (10), 3 beta-hydroxyazadiradione (11), 3 beta-16 beta-dihydroxyazadiradione (12), (3-H-2)-3 beta-hydroxyazadiradione (13), (3,16-H-2)-3 beta-16 beta-dihydroxyazadiradione (14), (1,3,7-H-2)-1,2-dihydro-3 beta-hydroxy-7-deacetylazadiradione (15), 1,2,20,21,22,23-hexahydroazadiradione (17), and (1,3-H-2)-1,2-dihydro-3 beta-hydroxygedunin (29). These limonoids along with their semisynthesized derivatives were subjected to ultra high performance liquid chromatography mass spectrometry (UHPLC-MS/MS) and the fragmentation pathway was established based on structure-fragment relationships (SFRs), utilizing high resolution MS/MS data. We have developed a most reliable and easily reproducible protocol describing in depth analysis of SFRs based on the structural modifications and synthesis of isotopologs. Also, the MS/MS fragment library of these basic limonoids generated in this study acts as a fingerprint for accurate identification and quantification of limonoids by MS/MS analysis in various plant tissue extracts, phytopharmaceutical formulations and biological samples.1 aMulani, Fayaj, A.1 aNandikol, Sharvani, S.1 aHaldar, Saikat1 a  uhttps://library.ncl.res.in/content/accurate-identification-bioactive-meliaceae-limonoids-uhplc-msms-based-structure-fragment