01418nas a2200145   4500008004100000245008900041210006900130260000800199300001200207490000600219520088100225100002501106700002101131856012001152       2013                        eng d00aGamma-glutamyl transferases: a structural, mechanistic and physiological perspective0 aGammaglutamyl transferases a structural mechanistic and physiolo  cJAN  a51–650 v93 a<p>Gamma glutamyl transferases (GGT) are highly conserved enzymes that occur from bacteria to humans. They remove terminal γ-glutamyl residue from peptides and amides. GGTs play an important role in the homeostasis of glutathione (a major cellular antioxidant) and in the detoxification of xenobiotics in mammals. They are implicated in diseases like diabetes, inflammation, neurodegenerative diseases and cardiovascular diseases. The physiological role of GGTs in bacteria is still unclear. Nothing is known about the basis for the strong conservation of the enzyme across the living system. The review focuses on the enzyme’s physiology, chemistry and structural properties of the enzyme with emphasis on the evolutionary relationships. The available data indicate that the members of the GGT family share common structural features but are functionally heterogenous.</p>1 aBalakrishna, Sharath1 aPrabhune, Asmita  uhttps://library.ncl.res.in/content/gamma-glutamyl-transferases-structural-mechanistic-and-physiological-perspective01673nas a2200181   4500008004100000022001400041245010700055210006900162260011000231300001400341490000600355520091600361100001701277700002101294700002901315700001901344856012801363       2013                        eng d  a2050-750X00aGlucose oxidase conjugated H2O2 sensitive CdTe QDs: an effective fluorescence tool for glucose sensing0 aGlucose oxidase conjugated H2O2 sensitive CdTe QDs an effective   aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcOCT  a6538-65430 v13 a<p>Water-soluble quantum dots (QDs) are extensively used for molecular sensing because of the flexibility they offer in terms of modification of the QDs surface with a variety of functional groups using thiol chemistry and monitoring by fluorescence intensity. We describe a simple assay that allows the photoluminescence (PL) detection of H2O2 and glucose in aqueous samples and demonstrate its applicability by estimating glucose in blood. To enable the glucose detection, we functionalized the 3-mercaptopropanoic acid (MPA) capped CdTe QDs with glucose oxidase (GOx), the enzyme specific to b-D-glucose, using carbodiimide chemistry. The fluorescence of the GOx-functionalized CdTe QDs was quenched on the interaction with glucose. The same photoluminescence quenching was also observed in gel form, when a GOx modified CdTe QDs loaded agarose gel was dipped in H2O2 and glucose solutions, respectively.</p>1 aSingh, Pooja1 aPrabhune, Asmita1 aOgale, Satishchandra, B.1 aGuin, Debanjan  uhttps://library.ncl.res.in/content/glucose-oxidase-conjugated-h2o2-sensitive-cdte-qds-effective-fluorescence-tool-glucose-002458nas a2200277   4500008004100000020001300041245007900054210006900133260000800202300000900210490000700219520159500226653002501821653002001846653001401866653003001880653002601910653002601936100002201962700002001984700001802004700002202022700002102044700001702065856009802082       2021                        eng d  a0971119800aGreen antibacterial molecules: sophorolipids with varying fatty acid chain0 aGreen antibacterial molecules sophorolipids with varying fatty a  cNOV  a431+0 v353 aAntibiotic resistance is a rising problem of over the last decade making it difficult to treat infections caused by various pathogenic bacteria. To tackle this problem, newer antimicrobial strategies are being explored. Sophorolipids (SLs) are a class of biomolecules produced by a non-pathogenic yeast Starmarella bombicola (S. bombicold) with surfactant as well as potent broad spectrum antibacterial activity. In this work, we have successfully synthesized sophorolipids using different fatty acids with increasing chain length of hydrophobic tail; Lauric acid (C12)-LASL, Myristic acid (C14)-MASL, Palmitic acid (C16)-PASL and Stearic acid (C18)-SASL. We have used various techniques like FTIR, HRMS to characterize the synthesized SL. Further, we have measured properties like the critical micellar concentration for these biosurfactants and the SL's showed CMC less than or equal to 100 mg/L indicating excellent surface-active property. We have studied the antibacterial activity against gram-negative and gram-positive bacteria. Interestingly, the antibacterial activity was found to be more potent as the chain length of the fatty acid in the SL decreased. This trend was found to be reverse for toxicity towards mammalian cells. The shorter chain fatty acid SL's were found to be less cytotoxic. These results indicate that the sophorolipid candidates with shorter chain lengths would be beneficial for various biomedical applications such as tissue engineering, regenerative medicine and wound healing. Keywords: sophorolipid, biosurfactant, short chain fatty acid, antibacterial10aAntibacterial agents10aCells (Biology)10aInfection10aMicrobial drug resistance10aSaturated fatty acids10aSurface active agents1 aHirlekar, Swarali1 aAbhyankar, Isha1 aKane, Kartiki1 aTrimukhe, Kalpana1 aPrabhune, Asmita1 aNisal, Anuya  uhttps://link.gale.com/apps/doc/A684660702/AONE?u=anon~d0cd1b4e&sid=googleScholar&xid=1a6d61d9