01591nas a2200277 4500008004100000022001400041245013500055210006900190260010600259300001400365490000700379520057400386653002300960653001400983653002700997653001801024653001401042653001701056100002001073700001701093700002001110700001801130700002001148700002101168856012401189 2006 eng d a0731-708500aEffect of calcination cycles on the preparation of tin oxide based traditional drug: studies on its formation and characterization0 aEffect of calcination cycles on the preparation of tin oxide bas aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcJUN a1473-14780 v413 a

The preparation method of metal based Indian traditional drugs involves conversion of a pure metal into its oxide by repeated high temperature calcination cycles. In this work, the effect of number of calcination cycles followed in the preparation of tin oxide based Ayurvedic drug, `vanga bhasma' was studied by a systematic characterization of the drug samples after various calcination stages. It was found that tin was in the form of Sn4+ state and that the formation of SnO2 proceeded step-wise through Sn(OH)(4). (c) 2006 Elsevier B.V. All rights reserved.

10acalcination cycles10aOxidation10aoxidation state of tin10aParticle size10atin oxide10avanga bhasma1 aWadekar, M., P.1 aRode, C., V.1 aBendale, Y., N.1 aPatil, K., R.1 aGaikwad, A., B.1 aPrabhune, Asmita uhttps://library.ncl.res.in/content/effect-calcination-cycles-preparation-tin-oxide-based-traditional-drug-studies-its-002078nas a2200253 4500008004100000022001400041245019000055210006900245260007900314300001200393490000800405520106000413653002801473653002501501653002401526653002101550653002701571100001601598700001801614700001901632700002101651700002001672856013201692 2009 eng d a0273-228900aEffect of cultural conditions and media constituents on production of penicillin V acylase and CTAB treatment to enhance whole-cell enzyme activity of rhodotorula aurantiaca (NCIM 3425)0 aEffect of cultural conditions and media constituents on producti a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcJUN a463-4720 v1573 a

Penicillin V acylase (PVA) is a pharmaceutically important enzyme as it plays a vital role in the manufacture of semi-synthetic beta-lactam antibiotics. Rhodotorula aurantiaca (NCIM 3425) produced high levels of intracellular penicillin V acylase after 18 h at pH 8.0 and temperature 27 degrees C. Fructose was the best carbon source for PVA production, whereas tryptone was the best nitrogen source to produce the enzyme up to 170 and 1,088 IU/l of culture, respectively. Additionally, the cell-bound PVA activity was enhanced on treatment with cationic detergent. Whole-cell activity was found to be doubled (204%) on treatment of 0.01 g dry weight of cells with 50 mu g/ml solution of N-cetyl-N,N,N-trimethylammoniumbromide at pH 8.0 for 1 h at room temperature. Atomic force microscopy images of permeabilized cells show perturbation in the cell wall and offer first-ever visual illustration of surface structure modifications that occur during permeabilization of R. aurantiaca cells leading to enhancement in activity of intracellular enzyme.

10aAtomic force microscopy10aIntracellular enzyme10aPenicillin Vacylase10aPermeabilization10aRhodotorula aurantiaca1 aKumar, Atul1 aSingh, Sanjay1 aPoddar, Pankaj1 aPrabhune, Asmita1 aPundle, Archana uhttps://library.ncl.res.in/content/effect-cultural-conditions-and-media-constituents-production-penicillin-v-acylase-and-ctab-002314nas a2200217 4500008004100000022001400041245015200055210006900207260005700276300001000333490000700343520147800350653001301828653001201841653001101853653003601864100001801900700002701918700002101945856013001966 2013 eng d a1590-426100aEvaluation of ethanol production by a new isolate of yeast during fermentation in synthetic medium and sugarcane bagasse hemicellulosic hydrolysate0 aEvaluation of ethanol production by a new isolate of yeast durin a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcMAR a63-700 v633 a

A new xylose fermenting yeast was isolated from over-ripe banana by enrichment in xylose-containing medium. The phylogenetic analysis of ITS1-5.8S-ITS2 region sequences of ribosomal RNA of isolate BY2 revealed that it shows affiliation to genus Pichia and clades with Pichia caribbica. In batch fermentation, Pichia strain BY2 fermented xylose, producing 15 g l(-1) ethanol from 30 g l(-1) xylose under shaking conditions at 28A degrees C, with ethanol yield of 0.5 g g(-1) and volumetric productivity of 0.31 g l(-1) h(-1). The optimum pH range for ethanol production from xylose by Pichia strain BY2 was 5-7. Pichia strain BY2 also produced 6.08 g l(-1) ethanol from 30 g l(-1) arabinose. Pichia strain BY2 can utilize sugarcane bagasse hemicellulose acid hydrolysate for alcohol production, efficiency of fermentation was improved by neutralization, and sequential use of activated charcoal adsorption method. Percent total sugar utilized and ethanol yield for the untreated hydrolysate was 17.14% w/v and 0.33 g g(-1), respectively, compared with 66.79% w/v and 0.45 g g(-1), respectively, for treated hemicellulose acid hydrolysate. This new yeast isolate showed ethanol yield of 0.45 g g(-1) and volumetric productivity of 0.33 g l(-1) h(-1) from sugarcane bagasse hemicellulose hydrolysate detoxified by neutralization and activated charcoal treatment, and has potential application in practical process of ethanol production from lignocellulosic hydrolysate.

10aD-xylose10aEthanol10aPichia10aSugarcane bagasse hemicellulose1 aHande, Aparna1 aMahajan, Siddharth, A.1 aPrabhune, Asmita uhttps://library.ncl.res.in/content/evaluation-ethanol-production-new-isolate-yeast-during-fermentation-synthetic-medium-and-001797nas a2200145 4500008004100000022001400041245009700055210006900152260000800221300001100229520125100240100002501491700002101516856011401537 2014 eng d a1537-744X00aEffect of pH on the hydrolytic kinetics of gamma-glutamyl transferase from bacillus subtilis0 aEffect of pH on the hydrolytic kinetics of gammaglutamyl transfe cFEB a2162703 a

The effect of pH on the steady state kinetics of gamma-glutamyl transferase (GGT) from Bacillus subtilis was examined using glutamyl-(3-carboxyl)-4-nitroanilide as the chromogenic reporter substrate. The enzyme was active in the pH range 7.0-11.0 with the optimum activity at pH 11.0. We noticed a pH dependent transformation in the nature of substrate consumption kinetics. The substrate saturation curves were hyperbolic in the pH range 7.0-9.0 but changed into sigmoid form at pH 10.0 and 11.0. Hill's coefficients were > 1. We also analysed the effect of pH on the structure of the enzyme. The circular dichroism spectra of the enzyme sample at pH 9.0 and 11.0 were coincidental in both far and near UV regions indicating conservation of the secondary and tertiary structures, respectively. Themolecular weight of the enzyme sample was the same in both pH 7.0 and 11.0 indicating conservation of the quaternary structure. These results show that the kinetic transformation does not involve significant conformational changes. Cooperative binding of multiple substrate molecules may not be the basis for the sigmoid kinetics as only one substrate binding site has been noticed in the reported crystal structures of B. subtilis GGT.

1 aBalakrishna, Sharath1 aPrabhune, Asmita uhttps://library.ncl.res.in/content/effect-ph-hydrolytic-kinetics-gamma-glutamyl-transferase-bacillus-subtilis02599nas a2200193 4500008004100000245011600041210006900157260000800226300001200234490000800246520190600254100001702160700001702177700002102194700001702215700002402232700002702256856012202283 2016 eng d00aEvaluating the anticancer activity and nanoparticulate nature of homeopathic preparations of Terminalia chebula0 aEvaluating the anticancer activity and nanoparticulate nature of cNOV a318-3260 v1053 aBackground: Breast cancer is the most common cancer diagnosed among women and is the second leading cause of cancer death. Homeopathic medicines are part of the alternative medicines that are given as a supportive therapy in breast cancer. The objective of this study was to investigate the anticancer activity of commercially available homeopathic preparations of Terminalia chebula (TC) and evaluate their nanoparticulate nature. Methods: Mother tincture (MT) and other homeopathic preparations (3X, 6C and 30C) of TC were tested for their effect on the viability of breast cancer (MDAMB231 and MCF7) and non-cancerous (HEK 293) cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell growth assay was performed to analyze the effect of the different potencies on the growth kinetics of breast cancer cells. MT and 6C were evaluated for the presence of nanoparticles by using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Results: MT decreased the viability of breast cancer (MDAMB231 and MCF7) and non-cancerous (HEK 293) cells. However, the other potencies (3X, 6C and 30C) decreased the viability of only breast cancer cells without affecting the viability of the non-cancerous cells. All the potencies, MT, 3X, 6C and 30C, reduced growth kinetics of breast cancer cells, more specifically at 1:10 dilution at 24, 48 and 72 h. Under SEM, MT appeared as a mesh-like structure whereas under TEM, it showed presence of nanoclusters. On the other hand, 6C potency contained 20 nm sized nanoparticles. Conclusion: The current study reports the anticancer activity of homeopathic preparations of TC against breast cancer and reveals their nanoparticulate nature. These preliminary results warrant further mechanistic studies at both in vitro and in vivo levels to evaluate the potential of TC as nanomedicine in breast cancer.1 aWani, Kirtee1 aShah, Nilesh1 aPrabhune, Asmita1 aJadhav, Arun1 aRanjekar, Prabhakar1 aKaul-Ghanekar, Ruchika uhttps://library.ncl.res.in/content/evaluating-anticancer-activity-and-nanoparticulate-nature-homeopathic-preparations03206nas a2200193 4500008004100000245011200041210006900153260000800222300001200230490000700242520252000249100001902769700002002788700001702808700002102825700001702846700002002863856012902883 2019 eng d00aEffect of media components and growth conditions for improved linoleic acid production by beauveria species0 aEffect of media components and growth conditions for improved li cAUG a945-9540 v963 a

Beauveria species are well-known insect pathogenic fungi, and Beauveria bassiana is used as a biopesticide against various pests in agriculture. However, the Beauveria species has not been reported as producers of microbial oils. In this study, Beauveria spp. MTCC 5184 was used to produce microbial oil with high linoleic acid (LA) content. Ten experiments were performed to evaluate the effects of several media parameters, such as carbon and nitrogen sources, pH, various concentrations of carbon and nitrogen, growth duration, and oleic acid (OLA) supplementation for maximum LA and dry biomass production by the fungus. Several of these parameters had a significant impact on the production of LA, as well as dry biomass. The glucose yeast extract (GYE) medium supplemented with 1.5% (w/v) peptone yielded maximum LA (0.32 +/- 0.01 g L-1) and biomass (5.51 +/- 0.26 g L-1). However, through the addition of 1.0% (w/v) OLA, the precursor of LA, LA production was enhanced 12-fold (1.24 +/- 0.03 g L-1), and the biomass production increased by 5-fold (11.05 +/- 0.46 g L-1) in comparison to those in the basal (GYE) medium. Using lactose as the sole carbon source produced the lowest LA (0.05 +/- 0.00 g L-1) and biomass (1.04 +/- 0.10 g L-1). The results of this study will be useful for the commercial exploitation of this fungus for the production of LA-rich microbial oil for use in the production of lubricants, greases, paints, cosmetics, etc.

1 aSanghi, Smrati1 aChirmade, Tejas1 aMore, Snehal1 aPrabhune, Asmita1 aGupta, Vidya1 aKadoo, Narendra uhttps://library.ncl.res.in/content/effect-media-components-and-growth-conditions-improved-linoleic-acid-production-beauveria02344nas a2200289 4500008004100000022001400041245008000055210006900135260000900204300001100213490000800224520144400232653002501676653003301701653001501734653001201749100002301761700002201784700002101806700001901827700002101846700002101867700001701888700002201905700002101927856010601948 2021 eng d a0959-652600aEnergy and life cycle impact assessment for xylitol production from corncob0 aEnergy and life cycle impact assessment for xylitol production f cJAN  a1232170 v2783 a

The conversion of biobased feedstocks into energy and value-added chemicals is one main approach to address the current global challenge for waste mitigation through the biorefinery mode. Corncob biomass which is one of the most abundant agricultural residue in India with a high hemicellulose content, can serve as a potential low-cost raw material for food-grade xylitol production. The present study demonstrates a process for xylitol production from corncob biomass with detailed energy and life cycle analysis for viability assessment. The major highlight of the process is the use of microbial fermentation, where biomass-derived D-xylose is converted to xylitol with high selectivity. Overall, 0.502 kg of xylitol crystals could be produced from 3.5 kg of corncob biomass. Simulation analysis revealed that the evaporators are the primary consumers of energy, and the process of heat integration can significantly reduce the energy requirements of the overall process. Environmental impacts of the system evaluated showed emission results of 8.68 kg CO2 equivalent and revealed that marine aquatic and freshwater eco-toxicity are the only possible contributors to the environment. The results suggest that the process would have favourable energy balances, which can be used in pilot plant and heat exchanger network design and operation for xylitol production scale up to 500 L. (C) 2020 Elsevier Ltd. All rights reserved.

10abiomass valorization10aLife cycle impact assessment10asimulation10axylitol1 aDasgupta, Diptarka1 aSidana, Arushdeep1 aGhosh, Prasenjit1 aSharma, Tripti1 aSingh, Jasvinder1 aPrabhune, Asmita1 aMore, Snehal1 aBhaskar, Thallada1 aGhosh, Debashish uhttps://library.ncl.res.in/content/energy-and-life-cycle-impact-assessment-xylitol-production-corncob01988nas a2200229 4500008004100000022001400041245009800055210006900153260000800222300001000230490000800240520120800248653001701456653003701473653001801510100002001528700001701548700002101565700002601586700002001612856012601632 2021 eng d a0022-263100aExpression, purification and crystallization of Asrij, A novel scaffold transmembrane protein0 aExpression purification and crystallization of Asrij A novel sca cFEB a65-740 v2543 a

Asrij/OCIAD1 is a scaffold transmembrane protein belonging to the Ovarian Carcinoma Immunoreactive Antigen Domain containing protein family. In Drosophila and mouse models, Asrij localizes at the endosomal and mitochondrial membrane and is shown to regulate the stemness of hematopoietic stem cells. Interaction of Asrij with ADP Ribosylation Factor 1 (Arf1) is shown to be crucial for hematopoietic niche function and prohemocyte maintenance. Here, we report the heterologous expression, standardization of detergents and purification methodologies for crystallization of Asrij/OCIAD1. To probe the activity of bacterially expressed Asrij, we developed a protein complementation assay and conclusively show that Asrij and Arf1 physically interact. Further, we find that sophorolipids improve the solubility and monodispersibility of Asrij. Hence, we propose that sophorolipids could be novel additives for stabilization of membrane proteins. To our knowledge, this is the first study detailing methodology for the production and crystallization of a heterologously expressed scaffold membrane protein and will be widely applicable to understand membrane protein structure and function. Graphic

10aAsrij/OCIAD110aMembrane protein crystallography10asophorolipids1 aMotiwala, Zenia1 aDarne, Priti1 aPrabhune, Asmita1 aInamdar, Maneesha, S.1 aKulkarni, Kiran uhttps://library.ncl.res.in/content/expression-purification-and-crystallization-asrij-novel-scaffold-transmembrane-protein