01963nas a2200229   4500008004100000022001400041245014700055210006900202260007300271300001200344490000800356520107500364653001201439653002501451653002201476100001901498700002001517700001901537700002501556700002201581856013001603       2007                        eng d  a0168-165600aCharacterization of the proteins of bacterial strain isolated from contaminated site involved in heavy metal resistance - a proteomic approach0 aCharacterization of the proteins of bacterial strain isolated fr  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcFEB  a444-4510 v1283 a<p>The present study describes response of a bacterial strain isolated from a polluted river to heavy metal toxicity. The bacterium was identified to be Klebsiella pneumoniae by biochemical tests using API 20E strips and 16S ribotyping. The isolate was studied for its tolerance to two heavy metals, i.e., cobalt (Co2+) and lead (Pb2+) by growing it in citrate mineral medium (CMM). Proteomic approach involving two-dimensional polyacrylamide gel electrophoresis (2D PAGE) and mass spectrometry (MS) was used to identify the differentially expressed proteins under heavy metal stress. Two of the differentially expressed proteins were identified to be L-isoaspartate protein carboxymethyltransferase type II and DNA gyrase A. To our knowledge, this is for the first time that K pneumoniae has been reported to be present in metal contaminated site and L-isoaspartate protein carboxymethyltransferase type II protein to be over expressed under heavy metal stress. The role of these proteins in metal tolerance is discussed. (c) 2006 Elsevier B.V. All rights reserved.</p>10a2D PAGE10aheavy metal toxicity10aMass spectrometry1 aBar, Chinmayee1 aPatil, Rajendra1 aDoshi, Jignesh1 aKulkarni, Mahesh, J.1 aGade, Wasudeo, N.  uhttps://library.ncl.res.in/content/characterization-proteins-bacterial-strain-isolated-contaminated-site-involved-heavy-metal