02382nas a2200313 4500008004100000022001400041245008600055210006900141260009200210300001400302490000600316520133700322653001701659653001901676653001501695653002001710653003301730653001301763653001401776100002401790700001901814700001901833700001901852700001901871700001601890700002001906700002801926856011401954 2016 eng d a1947-293500aAnti-oxidant properties of ficus religiosa L. Bark extract on human keratinocytes0 aAntioxidant properties of ficus religiosa L Bark extract on huma a26650 THE OLD RD, STE 208, VALENCIA, CA 91381-0751 USAbAMER SCIENTIFIC PUBLISHERScJUN a1221-12260 v83 a

Ficus religiosa (Sacred Fig) is a medicinally important tree, native to the Indian subcontinent. It has been extensively pharmacologically researched species having wide spectrum of medicinal properties. All parts of F. religiosa tree are known to possess important medicinal properties such as anti-oxidant, anti-inflammatory, wound healing and skin diseases etc. However, effects of F. religiosa on skin cells line HaCaT was not studied for its antioxidant properties. In this report we have investigated F. religiosa bark aqueous extract for its antioxidant properties on human keratinocytes (HaCaT) cell line using DPPH, superoxide dismutase 1, superoxide dismutase 2 and catalase assay. We observed that F. religiosa bark aqueous extract efficiently scavenged (80%) DPPH radicals. Superoxide dismutase1 assay of F. religiosa bark aqueous extract effectively scavenged superoxide radicals (O-2(-)) and showed dose dependent activity. Reactive oxygen species were trapped by superoxide dismutase 2 assay of F. religiosa bark aqueous extract and form hydrogen peroxide. Catalase assay results revelled that hydrogen peroxide was further decomposed to give water and oxygen. Thus various anti-oxidant assays of F. religiosa bark aqueous extract indicate that it efficiently reduced the reactive oxygen species in skin cells.

10aAnti-Oxidant10aCatalase Assay10aDPPH Assay10aFicus religiosa10aKeratinocyte HaCaT Cell Line10aMoraceae10aSOD Assay1 aDixit, Shailesh, S.1 aKulkarni, Atul1 aSeo, Hyo, Hyun1 aGadgil, Jayant1 aDingre, Medini1 aUmar, Ahmad1 aMoh, Sang, Hyun1 aParasharami, Varsha, A. uhttps://library.ncl.res.in/content/anti-oxidant-properties-ficus-religiosa-l-bark-extract-human-keratinocytes03778nas a2200217 4500008004100000245015200041210006900193260000800262300001200270490000700282520298400289100001703273700001903290700001603309700002103325700001903346700002003365700001903385700002803404856012803432 2020 eng d00aEnhanced anti-ageing and wound healing properties of Ficus religiosa L. bark, leaf and aerial root extract in human keratinocytes cell line (HaCaT)0 aEnhanced antiageing and wound healing properties of Ficus religi cJAN a158-1650 v333 a

Plants have played a vital role in curative and preventive healthcare. Ficus religiosa L., a medicinally important tree has been used as a traditional medicine to treat various ailments and skin diseases. However, its skin anti-ageing properties are not yet scientifically evaluated. Here we report the comparison of F. religiosa bark (FRAqB), leaf (FRAqL) and aerial-roots (FRAqAR) extracts for skin anti-ageing and wound healing properties. FRAqB, FRAqL and FRAqAR extracts preparation, cell culture, RNA isolation, real time PCR, astringent activity and wound healing assay were carried out using standard methods. Axio Observer FL-microscope and ImageJ software were used for wound healing assay analysis. The real time PCR study reveals that matrix metalloproteinase-1 (MMP) RNA was downregulated to 75% and 95% for FRAqB and FRAqAR, respectively. For procollagen C-endopeptidase enhancer 1 (PCOLCE), FRAqB and FRAqL enhanced RNA levels by 500% and 300%, respectively. FRAqB and FRAqL increased wound healing area to about 60% and FRAqAR by 50%. All three extracts demonstrated astringent activity which is significant for skin tightening. The study proves that extracts of F. religiosa are prominent candidates for pharmaceutical and cosmetic applications such as skin anti-ageing and wound healing.

1 aPandey, Prem1 aSeo, Hyo, Hyun1 aKim, Hye-In1 aRyu, Seung, Hwan1 aDingre, Medini1 aMoh, Sang, Hyun1 aKulkarni, Atul1 aParasharami, Varsha, A. uhttps://library.ncl.res.in/content/enhanced-anti-ageing-and-wound-healing-properties-ficus-religiosa-l-bark-leaf-and-aerial