02522nas a2200265 4500008004100000022001400041245007700055210006900132260007300201300001200274490000800286520164000294653001601934653000901950653001001959653001401969653002701983653002902010100002802039700001802067700001802085700001802103700002702121856010802148 2013 eng d a0927-776500aBiosynthesis of Se nanoparticles and its effect on UV-induced DNA damage0 aBiosynthesis of Se nanoparticles and its effect on UVinduced DNA aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAR a261-2660 v1033 a

This paper reports, an environmentally benign procedure of synthesis and characterizations of selenium nanoparticles and their protective effect against UV-induced DNA damage activities. An aqueous leaf extract of lemon plant was used as a precursor for synthesis of colloidal selenium nanoparticles. Resulting nanoparticles were characterized using UV-vis spectrophotometer, photoluminescence, TEM, EDAX, FT-IR and XRD, respectively. Selenium colloidal solution exhibited an absorption maximum at 395 nm and produced an emission maximum at 525 nm. Transmission electron microscopy followed by selected area electron diffraction pattern analysis indicated the formation of spherical, polydispersed, crystalline, selenium nanoparticles of diameter ranging from (similar to 60 to 80 nm). X-ray diffraction studies showed the formation of 111, 2 0 0 and 220 planes of face-centered cubic (fcc) selenium. EDAX analysis confirmed the presence of selenium in nanosphere. Fourier transformed infrared spectroscopic investigation reveled the involvement of carboxyl (-C=0), hydroxyl (-OH), amine (-NH) functional group of lemon plant extract in preparation of selenium nano particles. MU assay as well single cell gel electrophoresis assay or comet assay revealed that synthesized selenium nanoparticles, caused less cell death of lymphocytes and prevented DNA damage, when cells were exposed to UVB. The fluorescent property of selenium nanopartides can be used as diagnostic agent. Further, their anti DNA damaging property can be investigated as a chemotherapeutic agent in cancer therapy. (C) 2012 Elsevier B.V. All rights reserved.

10aComet assay10aEDAX10aFT-IR10aMTT assay10aSelenium nanoparticles10aUV-vis spectrophotometer1 aPrasad, Kumar, Suranjit1 aPatel, Hirnee1 aPatel, Tirtha1 aPatel, Khusbu1 aSelvaraj, Kaliaperumal uhttps://library.ncl.res.in/content/biosynthesis-se-nanoparticles-and-its-effect-uv-induced-dna-damage-001662nas a2200229 4500008004100000022001400041245011500055210006900170260007900239300001200318490000800330520080900338653001301147653001601160653000901176653001401185653002701199653002201226100002801248700002701276856012901303 2014 eng d a0163-498400aBiogenic synthesis of selenium nanoparticles and their effect on as(III)-induced toxicity on human lymphocytes0 aBiogenic synthesis of selenium nanoparticles and their effect on a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcMAR a275-2830 v1573 a

{A bioreductive capacity of a plant, Terminalia arjuna leaf extract, was utilized for preparation of selenium nanoparticles. The leaf extract worked as good capping as well as stabilizing agent and facilitated the formation of stable colloidal nanoparticles. Resulting nanoparticles were characterized using UV-Vis spectrophotometer, transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDAX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction analysis (XRD), respectively. The colloidal solution showed the absorption maximum at 390 nm while TEM and selected area electron diffraction (SAED) indicated the formation of polydispersed, crystalline selenium nanoparticles of size raging from 10 to 80 nm. FT-IR analysis suggested the involvement of O-H, N-H

10aArsenite10aComet assay10aEDAX10aMTT assay10aSelenium nanoparticles10aTerminalia arjuna1 aPrasad, Kumar, Suranjit1 aSelvaraj, Kaliaperumal uhttps://library.ncl.res.in/content/biogenic-synthesis-selenium-nanoparticles-and-their-effect-asiii-induced-toxicity-human-002204nas a2200301 4500008004100000022001400041245011000055210006900165260007900234300001400313490000800327520115400335653001401489653000901503653002201512653002701534653000801561653002401569100002801593700002601621700002201647700001801669700001901687700001801706700002101724700002701745856013001772 2015 eng d a0273-228900aMicrobial selenium nanoparticles (SeNPs) and their application as a sensitive hydrogen peroxide biosensor0 aMicrobial selenium nanoparticles SeNPs and their application as  a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcNOV a1386-13930 v1773 a

The cell-free extract, a crude enzyme (cytosolic and membrane fraction) obtained from an environmental isolate, Bacillus pumilus sp. BAB-3706 worked as excellent in reducing as well as stabilizing agent and facilitated the formation of stable colloidal selenium nanoparticles (SeNPs). Resulting nanoparticles were characterized using UV-vis spectrophotometer, TEM, EDAX, FT-IR and XRD, respectively. A working electrode was modified by coating the surface of indium tin oxide (ITO) with colloidal SeNPs. Successive additions of H2O2 (100 to 600 mu M) in conventional three electrodes system, cyclic voltammeter with potential scan rate 25.0 mV/s, in 0.1 M phosphate buffer solution (PBS) yielded increase in current. A perpetual amperometric response at fixed potential (-1.0 V) and at selected time interval of 100 s showed different magnitude of current at every addition of H2O2. The linear range of detection of H2O2 was from 5 to 600 mM (R (2) = 0.9965), while the calculated limit of detection was found to be 3.00 mu M. The current study suggested that microbial SeNPs can be used for fabrication of low cost, sensitive H2O2 biosensor.

10aBiosensor10aEDAX10aHydrogen peroxide10aSelenium nanoparticles10aTEM10aUV-vis spectroscopy1 aPrasad, Kumar, Suranjit1 aVaghasiya, Jayraj, V.1 aSoni, Saurabh, S.1 aPatel, Jitesh1 aPatel, Rinkesh1 aKumari, Madhu1 aJasmani, Falguni1 aSelvaraj, Kaliaperumal uhttps://library.ncl.res.in/content/microbial-selenium-nanoparticles-senps-and-their-application-sensitive-hydrogen-peroxide-002361nas a2200265 4500008004100000022001400041245013200055210006900187260000900256300001200265490000700277520148200284653001501766653001301781653000901794653001301803653000801816100002501824700001801849700002601867700002701893700001801920700002801938856012901966 2020 eng d a1060-132500aRemoval of fluoride from aqueous solution by mesoporous silica nanoparticles functionalized with chitosan derived from mushroom0 aRemoval of fluoride from aqueous solution by mesoporous silica n cSEP  a619-6270 v573 a

In the present study, chitosan functionalized mesoporous silica nanoparticles have been synthesized. Chitosan derived from an edible mushroom, Agaricus bisporus was used during synthesis of chitosan functionalized silica nanoparticles. The functionalized silica nanoparticles were subjected to fluoride sorption using a batch method which subsequently showed removal efficiency of 95% with maximum sorption capacity, 58.8 mg/g. The characterization of nanoparticles was carried out by SEM, EDAX, XRD and FTIR analysis respectively. EDAX analysis suggested that oxygen, 51.83% and silica 37.24% was main constituent of the functionalized nanomaterials. XRD yielded characteristic diffraction pattern corresponding to the lattice planes (100), (110), (112), (120), (200) and (220) respectively. Sorption data was used to study equilibrium isotherm namely Langmuir and Freundlich model. The adsorption capacity increased with temperature while kinetics studies revealed that the adsorption process followed a pseudo-second-order rate equation. The enthalpy change (Delta H) and entropy change (Delta S) was found to be -31.36 kJmol(-1) and -7.75 Jmol(-1) K-1, showing endothermic and spontaneous nature of the fluoride adsorption. Data suggested that the nature of adsorption belonged to chemisorptions. The overall results suggested that the synthesized nanoparticles showed strong and specific affinity for fluoride and could be excellent adsorbents for defluoridation.

10aAdsorption10aChitosan10aEDAX10aFluoride10aSEM1 aSrivastava, Anushree1 aKumari, Madhu1 aRamanathan, Alagappan1 aSelvaraj, Kaliaperumal1 aPrasad, Bablu1 aPrasad, Kumar, Suranjit uhttps://library.ncl.res.in/content/removal-fluoride-aqueous-solution-mesoporous-silica-nanoparticles-functionalized-chitosan