01928nas a2200241   4500008004100000022001400041245010600055210006900161260007400230300001200304490000800316520107400324653000901398653002201407653001501429653001301444100001601457700002401473700002401497700002001521700001801541856012701559       2010                        eng d  a0016-670700aUnderstanding the genetic structure of symplocos laurina wall. populations using nuclear gene markers0 aUnderstanding the genetic structure of symplocos laurina wall po  aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcFEB  a197-2100 v1383 a<p>To characterize the genetic diversity of present populations of Symplocos laurina, which grow in the montane forests in India, we analyzed the DNA sequences of a nuclear gene. Using the 881 bp sequence of cytosolic Glyceraldehyde-3-phosphate dehydrogenase gene, we detected 24 haplotypes among 195 individuals sampled from 14 populations. Two dominant haplotypes were distributed over the entire range of this species in India and several private haplotypes were found. Low genetic diversity within population, high differentiation, number of population specific haplotypes and deviation from neutral evolution characterized the present populations of S. laurina. An analysis of molecular variance indicated the presence of geographic structure within the haplotype distribution. The occurrence of S. laurina preglaciation in India is the most parsimonious explanation for the current geographic structure observed. The populations are presumably ancient and might have spread across its extant distribution range in India through a recent range expansion event.</p>10aGapC10aGenetic diversity10aGlaciation10aRefugium1 aBanu, Sofia1 aBhagwat, Rasika, M.1 aKadoo, Narendra, Y.1 aLagu, Meena, D.1 aGupta, V., S.  uhttps://library.ncl.res.in/content/understanding-genetic-structure-symplocos-laurina-wall-populations-using-nuclear-gene-002422nas a2200301   4500008004100000022001400041245011200055210006900167260000800236300001100244490000800255520141300263653002501676653001501701653002201716653001501738653002701753653002101780100002401801700002701825700002401852700002001876700002701896700002401923700002401947700002301971856012601994       2024                        eng d  a0926-669000aUnlocking the genetic and biotechnological potential of Gloriosa superba to enhance its alkaloid production0 aUnlocking the genetic and biotechnological potential of Gloriosa  cMAY  a1181440 v2113 a<p>
	Gloriosa superba is a medicinally important plant that produces the pharmaceutically precious colchicine molecule. Colchicine is being used for treating gout, for enhancing the production of interleukin-8 (inhibits human pancreatic cancer) and many other human ailments. Since its seeds and rhizomes (tuberous roots) are the chief sources of colchicine, pharmaceutical industries exploit the natural sources, thus demolishing its habitat. Chemo- and genetic diversity among the G. superba populations exist, but has not been exploited profitably. Scope exists for generating mutants using the chemical mutagens and also via breeding methods to generate superior varieties for commercial cultivation. Innovative methods for breaking seed dormancy and superior colchicine extraction, besides biotechnological interventions are imperative to save the biodiversity and to produce colchicine on a large scale. Biorhizomes produced in vitro hold great promise for biomanufacturing colchicine in bioreactors commercially. Since the biosynthetic pathway of colchicine is now known, isolation of the pathway genes and their heterologous overexpression or the application of synthetic biology methods needs to be explored in future for industrial manufacturing of colchicine. Advances made in the recent past in these fields of colchicine production from G. superba are critically evaluated in this review.</p>
10aBiosynthetic pathway10aColchicine10aGenetic diversity10aGlory lily10aMetabolite elicitation10aOmics approaches1 aMahajan, Yogesh, A.1 aShinde, Balkrishna, A.1 aShirke, Harshad, A.1 aGandra, Jawahar1 aSuravajhala, Prashanth1 aKishor, P., B. Kavi1 aKadoo, Narendra, Y.1 aNikam, Tukaram, D.  uhttps://library.ncl.res.in/content/unlocking-genetic-and-biotechnological-potential-gloriosa-superba-enhance-its-alkaloid