02113nas a2200169   4500008004100000245015300041210006900194260000800263300001400271490000700285520143100292100002501723700002201748700002401770700002101794856012801815       2016                        eng d00aSequence characterization and in silico structure prediction of fatty acid desaturases in linseed varieties with differential fatty acid composition0 aSequence characterization and in silico structure prediction of   cDEC  a4896-49060 v963 a<p>BACKGROUND: Linseed is the richest agricultural source of alpha-linolenic acid (ALA), an omega-3 fatty acid (FA) that offers several nutritional benefits. In the present study, sequence characterization of six desaturase genes (SAD1, SAD2, FAD2, FAD2-2, FAD3A and FAD3B) and 3D structure prediction of their proteins from ten Indian linseed varieties differing in ALA content were performed to determine whether the nucleotide and amino acid (AA) sequence variants have any functional implications in differential accumulation of ALA or other FAs in linseed. RESULTS: The SAD and FAD2 genes exhibited few sequence variations among the ten varieties, forming only one or two protein isoforms. In contrast, the FAD3A and FAD3B genes showed more sequence variations and three or four protein isoforms. Interestingly, the two high-ALA varieties NL260 and Padmini had the same FAD3B nucleotide and protein isoforms, which differed from all other varieties. Surprisingly, no AA changes altered the 3D structures of the desaturase proteins. CONCLUSION: Several nucleotide and AA sequence variations in desaturase genes were observed; however, they did not alter the 3D structure of any desaturase protein and were not correlated with FA levels among the ten linseed varieties, which had different ALA contents. This suggests a complex regulatory process of biosynthesis of FAs in linseed. (C) 2016 Society of Chemical Industry</p>1 aRajwade, Ashwini, V.1 aJoshi, Rakesh, S.1 aKadoo, Narendra, Y.1 aGupta, Vidya, S.  uhttps://library.ncl.res.in/content/sequence-characterization-and-silico-structure-prediction-fatty-acid-desaturases-linseed02611nas a2200241   4500008004100000245014200041210006900183260000800252300001100260490000700271520174900278653002202027653002402049653002102073653001802094653001602112100002802128700002102156700001702177700002402194700002402218856012702242       2023                        eng d00aSeasonal dynamics and tree foliar habit drive the rhizobacterial diversity in congeneric Ficus species from Northern Western Ghats, India0 aSeasonal dynamics and tree foliar habit drive the rhizobacterial  cDEC  a1007900 v283 a<p>
	Tree species, annual climatic cycle, and geochemical properties influence the tree rhizospheric microbiome. However, its understanding in the moist deciduous forest of Northern Western Ghats (NWG), India, is limited. Moreover, the effects of foliar habits of trees and seasonal variations on rhizobacterial communities across congeneric tree species are unexplored. Hence, we employed high throughput 16 S rRNA gene sequencing to examine the effects of foliar habits of congeneric tree species and seasonal influences on the rhizobacterial community. For this study, rhizospheric soil from Ficus arnottiana (deciduous) and Ficus talboti (evergreen) trees from NWG, India, was collected during three seasons (summer, monsoon, and winter) and subjected to high throughput sequencing. A total of 3031 amplicon sequence variants (ASV) were observed, of which 1257 and 1181 ASV were unique to F. arnottiana and F. talboti, respectively, and 593 ASV were common to both species. The phyla Actinobacteria, Proteobacteria, Acidobacteria, and Chloroflexi were ubiquitous and contributed &gt;90% to the rhizobacterial community. Some bacterial phyla were exclusively associated with either of the tree species and seasons. Different bacterial phyla performed similar functions in different seasons, indicating their functional redundancy and emphasizing their resilience and adaptability across seasons. It was observed that seasonal dynamics mainly drove the rhizobacterial diversity; however, it was also influenced by the foliar habits of the trees and geochemical properties. The study enhances the understanding of congeneric tree microbiomes and offers valuable insights into their potential applications through microbiome engineering.</p>
10aAmplicon variants10aBacterial diversity10aFicus arnottiana10aFicus talboti10aRhizosphere1 aSalunkhe, Vaishnavi, H.1 aKunte, Pooja, S.1 aPaul, Dhiraj1 aKasodekar, Amol, K.1 aKadoo, Narendra, Y.  uhttps://library.ncl.res.in/content/seasonal-dynamics-and-tree-foliar-habit-drive-rhizobacterial-diversity-congeneric-ficus