02454nas a2200265   4500008004100000022001400041245010500055210006900160260007400229300001400303490000800317520151000325653002601835653002201861653000901883653001601892653002201908100002401930700001601954700002601970700002401996700002002020700002102040856012702061       2014                        eng d  a0378-269700aEvaluation of genetic variability in Symplocos laurina Wall. from two biodiversity hotspots of India0 aEvaluation of genetic variability in Symplocos laurina Wall from  aSACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIAbSPRINGER WIENcDEC  a2239-22470 v3003 a<p>Symplocos laurina Wall., an important medicinal tree species, is one of the pioneering plants for establishment of shola forest ecosystem. We analyzed 208 accessions of S. laurina from 13 populations belonging to three ecoregions in India namely, Western Ghats, Eastern Ghats and Northeast India, to comment on the genetic diversity and population structure of the species. Of the 100 inter simple sequence repeat primers screened, eight were selected based on their clear and reproducible polymorphic patterns. These eight ISSR primers produced on an average 1,014 bands from 208 accessions, of which 6.58 % were rare bands, 55.96 % were shared bands and 37.46 % were similar bands. The primers, UBC835 and UBC880, amplified the highest number of polymorphic loci (16), while the primer UBC852 amplified the least number of amplicons (9). The values for total genetic diversity (H-T) and the average heterozygosity (H-S) were 0.3407 and 0.2263, respectively; while the coefficient of genetic differentiation (G(ST)) was 0.3358. Within-population variance was higher (68 %) than among-population variance (17 %), indicating high intra-population genetic diversity. These populations were grouped according to their geographical location in cluster analysis. Mantel test also showed significant positive correlation between genetic and geographical distances. The present study revealed the importance of the Palghat gap in South India in shaping the distribution of genetic diversity in S. laurina.</p>10aBiodiversity hotspots10aGenetic diversity10aISSR10aPalghat gap10aSymplocos laurina1 aBhagwat, Rasika, M.1 aBanu, Sofia1 aDholakia, Bhushan, B.1 aKadoo, Narendra, Y.1 aLagu, Meena, D.1 aGupta, Vidya, S.  uhttps://library.ncl.res.in/content/evaluation-genetic-variability-symplocos-laurina-wall-two-biodiversity-hotspots-india-002219nas a2200217   4500008004100000022001400041245014700055210006900202260011500271300001200386490000700398520132900405653002501734653001901759100001701778700001601795700001801811700002401829700001801853856013001871       2015                        eng d  a0006-313400aExpression of stable reference genes and SPINDLY gene in response to gibberellic acid application at different stages of grapevine development0 aExpression of stable reference genes and SPINDLY gene in respons  aROZVOJOVA 263, PRAHA 6, CZ-165 02, CZECH REPUBLICbACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANYcSEP  a436-4440 v593 a<p>Gibberellic acid (GA(3)) is widely used at different stages of berry development, and to understand the molecular mechanism of its action requires identification of stable reference genes. We sprayed grapevine (Vitis vinifera L.) cv. Thompson Seedless with GA(3) at rachis stage for rachis elongation, at flower cluster stage for flower thinning, and at 3 - 4 mm berry stage for berry elongation. Tissue samples were collected at different time points after GA(3) application. The expression of 10 candidate reference genes was analyzed using 4 different algorithms to assess their suitability for real time-PCR data normalization. Based on the overall ranking, PP2A, Sutra, and SAND were identified as the most stably expressed genes across all samples. With regard to different stages, tubulin, EF1 alpha, and UBC were the most stable genes during rachis elongation; PP2A, SAND, and Sutra were the most suitable at the flower cluster and berry stages. The expression of GA signaling gene SPINDLY (VvSpy) was analyzed to validate the stable reference genes. After the GA(3) application, the expression of VvSpy was reduced at the rachis stage but did not change at the flower cluster and berry stages. The expression profile of VvSpy was comparable when two or three reference genes were used for data normalization.</p>10adevelopmental stages10aVitis vinifera1 aUpadhyay, A.1 aJogaiah, S.1 aMaske, S., R.1 aKadoo, Narendra, Y.1 aGupta, V., S.  uhttps://library.ncl.res.in/content/expression-stable-reference-genes-and-spindly-gene-response-gibberellic-acid-application-0