01648nas a2200217   4500008004100000022001400041245015200055210006900207260008000276300001000356490000600366520080400372653002101176653001201197653001801209100001801227700002601245700002001271700001801291856012101309       2010                        eng d  a1835-269300aDifferential accumulation of gliadin proteins in wheat grain of RILs grown at two different agroclimatic conditions and their effect on loaf volume0 aDifferential accumulation of gliadin proteins in wheat grain of   a8 91-93 MCKENZIE ST, LISMORE, NSW 2480, AUSTRALIAbSOUTHERN CROSS PUBLcJAN  a63-670 v43 a<p>The end use of wheat depends on the quality and quantity of protein accumulated in the grain during the grain filling stage. Gliadin families of proteins are proved to affect the bread making quality (BMQ) by affecting the rheology and functionality of dough. The gliadin accumulation was analysed using Indian genotypes grown in two diverse agro climatic zones. About 16 RILs along with their parents HI977 and HD2329 revealed significant difference in accumulation of all gliadin fractions. High accumulation of alpha and beta gliadin with low level of gamma gliadin was observed for Kota as compared to Pune location. However, this significant difference in gliadin accumulation due to environment, did not affect the loaf volume performance of the RILs at statistically significant level.</p>10aloaf volume (Lv)10aRP-HPLC10aWheat protein1 aElangovan, M.1 aDholakia, Bhushan, B.1 aGaikwad, S., M.1 aGupta, V., S.  uhttps://library.ncl.res.in/content/differential-accumulation-gliadin-proteins-wheat-grain-rils-grown-two-different-002492nas a2200229   4500008004100000245017500041210006900216260000800285300001200293490000700305520162800312100002701940700002601967700002001993700002002013700001602033700002002049700001902069700002002088700002402108856013002132       2017                        eng d00a Dynamic metabolic reprogramming of steroidal glycol-alkaloid and phenylpropanoid biosynthesis may impart early blight resistance in wild tomato (Solanum arcanum Peralta)0 aDynamic metabolic reprogramming of steroidal glycolalkaloid and   cNOV  a411-4230 v953 aAlternaria solani severely affects tomato (Solanum lycopersicum L.) yield causing early blight (EB) disease in tropical environment. Wild relative, Solanum arcanum Peralta could be a potential source of EB resistance; however, its underlying molecular mechanism largely remains unexplored. Hence, non-targeted metabolomics was applied on resistant and susceptible S. arcanum accessions upon A. solani inoculation to unravel metabolic dynamics during different stages of disease progression. Total 2047 potential metabolite peaks (mass signals) were detected of which 681 and 684 metabolites revealed significant modulation and clear differentiation in resistant and susceptible accessions, respectively. Majority of the EB-triggered metabolic changes were active from steroidal glycol-alkaloid (SGA), lignin and flavonoid biosynthetic pathways. Further, biochemical and gene expression analyses of key enzymes from these pathways positively correlated with phenotypic variation in the S. arcanum accessions indicating their potential role in EB. Additionally, transcription factors regulating lignin biosynthesis were also up-regulated in resistant plants and electrophoretic mobility shift assay revealed sequence-specific binding of rSaWRKY1 with MYB20 promoter. Moreover, transcript accumulation of key genes from phenylpropanoid and SGA pathways along with WRKY and MYB in WRKY1 transgenic tomato lines supported above findings. Overall, this study highlights vital roles of SGAs as phytoalexins and phenylpropanoids along with lignin accumulation unrevealing possible mechanistic basis of EB resistance in wild tomato.1 aShinde, Balkrishna, A.1 aDholakia, Bhushan, B.1 aHussain, Khalid1 aPanda, Sayantan1 aMeir, Sagit1 aRogachev, Ilana1 aAharoni, Asaph1 aGiri, Ashok, P.1 aKamble, Avinash, C.  uhttps://library.ncl.res.in/content/dynamic-metabolic-reprogramming-steroidal-glycol-alkaloid-and-phenylpropanoid-biosynthesis02365nas a2200301   4500008004100000022001400041245011000055210006900165260000800234300001200242490000700254520141200261653001301673653001001686653001601696653002301712653002101735653001501756653001701771100001601788700002601804700002501830700002101855700002401876700002001900700002001920856012301940       2020                        eng d  a1073-608500aDifferential modulation in metabolites revealed with the improvement in the shelf-life of alphonso fruits0 aDifferential modulation in metabolites revealed with the improve  cOCT  a508-5200 v623 a<p>Globally farmers have difficulty in extending the shelf-life of the tropical fruits due to their perishable nature. The present study aimed to assess the effect of hexanal nano-formulation treatment (NFT) on the shelf-life of Alphonso mango. Further, volatilomics was performed to explore the molecular basis of such effect. Untreated and treated fruits were sampled starting from 5th to 21st day after NFT at an interval of 4 days. Moderate changes in visual and digital colour parameters were evident from the intact and dissected fruits of NFT set compared to untreated fruits. Biochemical assays affirmed the phenotypic differences with significant changes in the colour imparting compounds like carotenoids and anthocyanins among them. Further, gas chromatography-mass spectrometry analysis revealed significant qualitative and quantitative variations in the different classes of compounds like lactones, furanones, esters, aldehydes and alcohols. Some of the key metabolites showed differential modulations among the NFT and untreated fruit sets indicating their potential role in various processes, which ultimately might have resulted in delayed ripening of the mango. Overall, this study has demonstrated the beneficial effect of hexanal and identified important metabolites with the enhanced shelf-life in Alphonso that could be useful for farmers and mango-based food/flavour industries.</p>
10aAlphonso10aGC-MS10aMetabolites10aNano-biotechnology10aNano-formulation10aShelf-life10aVolatilomics1 aDar, Saleem1 aDholakia, Bhushan, B.1 aShanmugam, Haripriya1 aGupta, Vidya, S.1 aSubramanian, K., S.1 aSubramanian, J.1 aGiri, Ashok, P.  uhttps://library.ncl.res.in/content/differential-modulation-metabolites-revealed-improvement-shelf-life-alphonso-fruits