01928nas a2200241   4500008004100000022001400041245010600055210006900161260007400230300001200304490000800316520107400324653000901398653002201407653001501429653001301444100001601457700002401473700002401497700002001521700001801541856012701559       2010                        eng d  a0016-670700aUnderstanding the genetic structure of symplocos laurina wall. populations using nuclear gene markers0 aUnderstanding the genetic structure of symplocos laurina wall po  aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcFEB  a197-2100 v1383 a<p>To characterize the genetic diversity of present populations of Symplocos laurina, which grow in the montane forests in India, we analyzed the DNA sequences of a nuclear gene. Using the 881 bp sequence of cytosolic Glyceraldehyde-3-phosphate dehydrogenase gene, we detected 24 haplotypes among 195 individuals sampled from 14 populations. Two dominant haplotypes were distributed over the entire range of this species in India and several private haplotypes were found. Low genetic diversity within population, high differentiation, number of population specific haplotypes and deviation from neutral evolution characterized the present populations of S. laurina. An analysis of molecular variance indicated the presence of geographic structure within the haplotype distribution. The occurrence of S. laurina preglaciation in India is the most parsimonious explanation for the current geographic structure observed. The populations are presumably ancient and might have spread across its extant distribution range in India through a recent range expansion event.</p>10aGapC10aGenetic diversity10aGlaciation10aRefugium1 aBanu, Sofia1 aBhagwat, Rasika, M.1 aKadoo, Narendra, Y.1 aLagu, Meena, D.1 aGupta, V., S.  uhttps://library.ncl.res.in/content/understanding-genetic-structure-symplocos-laurina-wall-populations-using-nuclear-gene-002454nas a2200265   4500008004100000022001400041245010500055210006900160260007400229300001400303490000800317520151000325653002601835653002201861653000901883653001601892653002201908100002401930700001601954700002601970700002401996700002002020700002102040856012702061       2014                        eng d  a0378-269700aEvaluation of genetic variability in Symplocos laurina Wall. from two biodiversity hotspots of India0 aEvaluation of genetic variability in Symplocos laurina Wall from  aSACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIAbSPRINGER WIENcDEC  a2239-22470 v3003 a<p>Symplocos laurina Wall., an important medicinal tree species, is one of the pioneering plants for establishment of shola forest ecosystem. We analyzed 208 accessions of S. laurina from 13 populations belonging to three ecoregions in India namely, Western Ghats, Eastern Ghats and Northeast India, to comment on the genetic diversity and population structure of the species. Of the 100 inter simple sequence repeat primers screened, eight were selected based on their clear and reproducible polymorphic patterns. These eight ISSR primers produced on an average 1,014 bands from 208 accessions, of which 6.58 % were rare bands, 55.96 % were shared bands and 37.46 % were similar bands. The primers, UBC835 and UBC880, amplified the highest number of polymorphic loci (16), while the primer UBC852 amplified the least number of amplicons (9). The values for total genetic diversity (H-T) and the average heterozygosity (H-S) were 0.3407 and 0.2263, respectively; while the coefficient of genetic differentiation (G(ST)) was 0.3358. Within-population variance was higher (68 %) than among-population variance (17 %), indicating high intra-population genetic diversity. These populations were grouped according to their geographical location in cluster analysis. Mantel test also showed significant positive correlation between genetic and geographical distances. The present study revealed the importance of the Palghat gap in South India in shaping the distribution of genetic diversity in S. laurina.</p>10aBiodiversity hotspots10aGenetic diversity10aISSR10aPalghat gap10aSymplocos laurina1 aBhagwat, Rasika, M.1 aBanu, Sofia1 aDholakia, Bhushan, B.1 aKadoo, Narendra, Y.1 aLagu, Meena, D.1 aGupta, Vidya, S.  uhttps://library.ncl.res.in/content/evaluation-genetic-variability-symplocos-laurina-wall-two-biodiversity-hotspots-india-0