01154nas a2200217 4500008004100000022001400041245010200055210006900157260000900226300001600235490000600251520040600257653001700663653002800680100002300708700002000731700002200751700001900773700002400792856012000816 2019 eng d a2365-654900aAcidic handle assemble heterogeneous carbocatalyst for facile aliphatic nucleophilic fluorination0 aAcidic handle assemble heterogeneous carbocatalyst for facile al cSEP  a10960-109640 v43 a

Acidic handle (-SO3H) assemble carbonaceous catalyst synthesized from glucose acts as a modulator for the nucleophilic fluorination reaction. The heterogeneous catalyst works with various alkyl sulfonate, halide and metal fluoride (MF), to offer a high yield of fluorinated products up to 93%. This carbonaceous catalyst is recyclable for more than five times, with no major loss of activity.

10afluorination10aheterogeneous catalysis1 aSaid, Madhukar, S.1 aPandole, Satish1 aSuryavanshi, Amol1 aKumar, Pradeep1 aGajbhiye, Jayan, M. uhttps://library.ncl.res.in/content/acidic-handle-assemble-heterogeneous-carbocatalyst-facile-aliphatic-nucleophilic02270nas a2200241 4500008004100000245007000041210006900111260000900180300001600189490000700205520148900212100002201701700002301723700002401746700001601770700002001786700002801806700002501834700002301859700002101882700002301903856010201926 2020 eng d00aApproach to nigericin derivatives and their therapeutic potential0 aApproach to nigericin derivatives and their therapeutic potentia cNOV  a43085-430910 v103 a

A new nigericin analogue that has been chemically modified was synthesized through a fluorination process from the parent nigericin, produced from a novel Streptomyces strain DASNCL-29. Fermentation strategies were designed for the optimised production of nigericin molecule and subjected for purification and structural analysis. The fermentation process resulted in the highest yield of nigericin (33% (w/w)). Initially, nigericin produced from the strain DASNCL-29 demonstrated polymorphism in its crystal structure, i.e., monoclinic and orthorhombic crystal lattices when crystallised with methanol and hexane, respectively. Furthermore, nigericin produced has been subjected to chemical modification by fluorination to enhance its efficacy. Two fluorinated analogues revealed that they possess a very potent antibacterial activity against Gram positive and Gram negative bacteria. To date, the nigericin molecule has not been reported for any reaction against Gram-negative bacteria, which are increasingly becoming resistant to antibiotics. For the first time, fluorinated analogues of nigericin have shown promising activity. In vitro cytotoxicity analysis of fluorinated analogues demonstrated tenfold lesser toxicity than the parent nigericin. This is the first type of study where the fluorinated analogues of nigericin showed very encouraging activity against Gram-negative organisms; moreover, they can be used as a candidate for treating many serious infections.

1 aSahu, Amit, Kumar1 aSaid, Madhukar, S.1 aHingamire, Tejashri1 aGaur, Megha1 aKhan, Abujunaid1 aShanmugam, Dhanasekaran1 aBarvkar, Vitthal, T.1 aDharne, Mahesh, S.1 aBharde, Atul, A.1 aDastager, Syed, G. uhttps://library.ncl.res.in/content/approach-nigericin-derivatives-and-their-therapeutic-potential01730nas a2200241 4500008004100000022001400041245011000055210006900165260000800234300001200242490000700254520094300261653000601204653000601210653002501216653001601241653001101257653002401268100002301292700002301315700002301338856012701361 2022 eng d a1139-670900aAntifungal compound from marine Serratia marcescens BKACT and its potential activity against Fusarium sp.0 aAntifungal compound from marine Serratia marcescens BKACT and it cNOV a851-8620 v253 a

Ecofriendly biocontrol agents to control pathogenic fungi are in demand globally. The present study evaluated the antifungal potentials of marine bacteria Serratia marcescens BKACT against eight different Fusarium species. A highest 75.5 +/- 0.80% of mycelial inhibition was observed against Fusarium foetens NCIM 1330. Structural characterization of the purified compound was analyzed by GC-MS and NMR techniques; based on the analysis, it is confirmed as 2, 4-di-tert butyl phenol (2, 4-DTBP) with chemical structure C14H22O. At 0.53 mM concentration, purified compound inhibited complete spore germination of F. foetens NCIM 1330. In vitro assay showed complete inhibition of F. foetens NCIM 1330 on the wheat seeds. Tested concentration does not show any toxic effect on germination of the seeds. By this study, we conclude that, 2, 4-DTBP is a suitable candidate to be used as biocontrol agent against Fusarium infection.

10a210a410adi-tert butyl phenol10aFusarium sp10amarine10aSerratia marcescens1 aKharat, Bharat, A.1 aSaid, Madhukar, S.1 aDastager, Syed, G. uhttps://library.ncl.res.in/content/antifungal-compound-marine-serratia-marcescens-bkact-and-its-potential-activity-against02778nas a2200325 4500008004100000245012400041210006900165260000800234300000700242490000700249520169500256653002401951653002001975653002701995653002302022653001702045653001402062653002002076653002802096100002202124700002202146700002202168700002302190700002602213700001902239700002202258700001802280700002202298856013202320 2023 eng d00aAntiviral and ROS scavenging potential of Carica papaya Linn and Psidium guajava leaves extract against HIV-1 infection0 aAntiviral and ROS scavenging potential of Carica papaya Linn and cMAR a820 v233 a

Antiretroviral therapy is the only treatment option for HIV-infected patients; however, it has certain drawbacks in terms of developing multiple toxic side effects. Thus, there is a continuous need to explore safe and efficacious anti-retroviral agents. Carica papaya Linn and Psidium guajava are known for their various biological activities. In this study, we characterized the bioactive fractions of methanolic leaves extract from both plants using the High-resolution electrospray ionization mass spectrometry (HR-ESI-MS) technique, followed by the investigation of their potential as anti-HIV-1 and antioxidant agents through in vitro mechanistic assays. The anti-HIV-1 activity was examined in TZM-bl cells through luciferase gene assay against two different clades of HIV-1 strains, whereas the intracellular ROS generation was analyzed by Fluorescence-Activated Cell Sorting. Additionally, the mechanisms of action of these phyto-extracts were determined through the Time-of-addition assay. The characterization of Carica papaya Linn and Psidium guajava leaves extract through HR-ESI-MS fragmentation showed high enrichment of various alkaloids, glycosides, lipids, phenolic compounds, terpenes, and fatty acids like bioactive constituents. Both the phyto-extracts were found to be less toxic and exhibited potent antiviral activity against HIV-1 strains. Furthermore, the phyto-extracts also showed a decreased intracellular ROS in HIV-1 infected cells due to their high antioxidant potential. Overall, our study suggests the anti-HIV-1 potential of Carica papaya Linn and Psidium guajava leaves extract due to the synergistic action of multiple bioactive constituents.

10aanti-HIV-1 activity10aAnti-retroviral10aBioactive constituents10aCarica papaya Linn10aCytotoxicity10aHR-ESI-MS10aPsidium guajava10areactive oxygen species1 aJadaun, Pratiksha1 aShah, Prachibahen1 aHarshithkumar, R.1 aSaid, Madhukar, S.1 aBhoite, Shubhangi, P.1 aBokuri, Sowmya1 aRavindran, Selvan1 aMishra, Neetu1 aMukherjee, Anupam uhttps://library.ncl.res.in/content/antiviral-and-ros-scavenging-potential-carica-papaya-linn-and-psidium-guajava-leaves-extract