01609nas a2200229   4500008004100000022001400041245010700055210006900162260011000231300001400341490000600355520070600361100002501067700002101092700002201113700001901135700002601154700002301180700002501203700002401228856012701252       2008                        eng d  a1477-052000aSynthesis and biological evaluation of bile acid dimers linked with 1,2,3-triazole and bis-beta-lactam0 aSynthesis and biological evaluation of bile acid dimers linked w  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcOCT  a3823-38300 v63 a<p>We report herein the synthesis and biological evaluation of bile acid dimers 11-18 linked through 1,2,3-triazole and bis-beta-lactam. The dimers 11-18 were synthesized using 1,3-dipolar cycloaddition reaction of diazido bis-beta-lactams 3, 4 and terminal alkynes 7-10 derived from cholic acid/deoxycholic acid in the presence of Cu(I) catalyst (click chemistry). These novel molecules were evaluated in vitro for their antifungal and antibacterial activity. Most of the compounds exhibited significant antifungal as well as antibacterial activity against all the tested fungal and bacterial strains. Moreover, their in vitro cytotoxicities towards HEK-293 and MCF-7 cells were also established.</p>1 aVatmurge, Namdev, S.1 aHazra, Braja, G.1 aPore, Vandana, S.1 aShirazi, Fazal1 aDeshpande, Mukund, V.1 aKadreppa, Sreenath1 aChattopadhyay, Samit1 aGonnade, Rajesh, G.  uhttps://library.ncl.res.in/content/synthesis-and-biological-evaluation-bile-acid-dimers-linked-123-triazole-and-bis-beta-002034nas a2200313   4500008004100000022001400041245010000055210006900155260010600224300001400330490000700344520092600351653002701277653001601304653001701320653001401337653001701351100002401368700002401392700002101416700002201437700002101459700002101480700001901501700002601520700002301546700002501569856012601594       2008                        eng d  a0960-894X00aSynthesis of chimeric tetrapeptide-linked cholic acid derivatives: impending synergistic agents0 aSynthesis of chimeric tetrapeptidelinked cholic acid derivatives  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcOCT  a5512-55170 v183 a<p>Tetrapeptides derived from glycine and beta-alanine were hooked at the C-3 beta position of the modified cholic acid to realize novel linear tetrapeptide-linked cholic acid derivatives. All the synthesized compounds were tested against a wide variety of microorganisms (Gram-negative bacteria, Gram-positive bacteria and fungi) and their cytotoxicity was evaluated against human embryonic kidney (HEK293) and human mammary adenocarcinoma (MCF-7) cell lines. While relatively inactive by themselves, these compounds interact synergistically with antibiotics such as fluconazole and erythromycin to inhibit growth of fungi and bacteria, respectively, at 1-24 mu g/mL. The synergistic effect shown by our novel compounds is due to their inherent amphiphilicity. The fractional inhibitory concentrations reported are comparable to those reported for Polymyxin B derivatives. (c) 2008 Elsevier Ltd. All rights reserved.</p>10aAntimicrobial activity10aCholic acid10aCytotoxicity10aSynergism10aTetrapeptide1 aBavikar, Sudhir, N.1 aSalunke, Deepak, B.1 aHazra, Braja, G.1 aPore, Vandana, S.1 aDodd, Robert, H.1 aThierry, Josiane1 aShirazi, Fazal1 aDeshpande, Mukund, V.1 aKadreppa, Sreenath1 aChattopadhyay, Samit  uhttps://library.ncl.res.in/content/synthesis-chimeric-tetrapeptide-linked-cholic-acid-derivatives-impending-synergistic-001196nas a2200205   4500008004100000022001400041245014800055210006900203260011000272300001400382490000700396520032900403100002300732700002300755700001900778700002100797700002500818700001700843856013000860       2014                        eng d  a1359-734500aNovel fluorescence probe for estimation of cysteine/histidine in human blood plasma and recognition of endogenous cysteine in live Hct116 cells0 aNovel fluorescence probe for estimation of cysteinehistidine in   aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcSEP  a9899-99020 v503 a<p>A new Cu(II)-complex is used as a ``Turn-On'' luminescence probe for specific detection of endogenous Cys in live Hct116 cells and Cys present in human blood plasma without any interference from other amino acids, especially GSH and Hcy. Difference in the mechanistic pathway for Cys and His recognition is discussed.</p>1 aReddy, Upendar, G.1 aAgarwalla, Hridesh1 aTaye, Nandaraj1 aGhorai, Suvankar1 aChattopadhyay, Samit1 aDas, Amitava  uhttps://library.ncl.res.in/content/novel-fluorescence-probe-estimation-cysteinehistidine-human-blood-plasma-and-recognition-001077nas a2200205   4500008004100000022001400041245017300055210006900228260011000297300001600407490000700423520019400430100002300624700001600647700001500663700001900678700002500697700001700722856013200739       2014                        eng d  a1359-734500aSpecific probe for Hg2+ to delineate even H+ in pure aqueous buffer/Hct116 colon cancer cells: Hg(II)-eta(2)-arene pi-interaction and a TBET-based fluorescence response0 aSpecific probe for Hg2 to delineate even H in pure aqueous buffe  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcSEP  a14421-144240 v503 a<p>A new molecular probe that demonstrates a distinct TBET process, induced by the Hg(II)-eta(2)-arene pi-interaction, in pure aqueous medium with a large pseudo-Stokes shift of 200 nm.</p>1 aReddy, Upendar, G.1 aRamu, Vadde1 aRoy, Sovan1 aTaye, Nandaraj1 aChattopadhyay, Samit1 aDas, Amitava  uhttps://library.ncl.res.in/content/specific-probe-hg2-delineate-even-h-pure-aqueous-bufferhct116-colon-cancer-cells-hgii-eta2-001104nas a2200205   4500008004100000022001400041245009200055210006900147260000800216300001600224490000700240520042300247100001500670700001800685700001900703700002400722700002500746700001700771856011000788       2015                        eng d  a1359-734500aFluorescent probe for specific detection of cysteine in the lipid dense region of cells0 aFluorescent probe for specific detection of cysteine in the lipi  cSEP  a16932-169350 v513 a<p>A new cysteine (Cys) specific chemodosimetric reagent (ER-F) is used in imaging of endogenous Cys localized in the lipid dense region of the live Hct116 cells and the release of Cys within HepG2 cells from a drug following a biochemical transformation. A silica surface, modified with ER-F, could be used for quantitative estimation of Cys present in aqueous solution (pH 7.2) and in a human blood plasma (HBP).</p>1 aAli, Firoj1 aAnila, H., A.1 aTaye, Nandaraj1 aGonnade, Rajesh, G.1 aChattopadhyay, Samit1 aDas, Amitava  uhttps://library.ncl.res.in/content/fluorescent-probe-specific-detection-cysteine-lipid-dense-region-cells01065nas a2200205   4500008004100000022001400041245008500055210006900140260007100209300001400280490000700294520032900301100002300630700001800653700001500671700001900686700002500705700001700730856011200747       2015                        eng d  a1523-706000aFRET-based probe for monitoring pH changes in lipid-dense region of Hct116 cells0 aFRETbased probe for monitoring pH changes in lipiddense region o  a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcNOV  a5532-55350 v173 a<p>A rhodamine conjugate (L) with a pseudo Stokes shift of 165 nm is used for probing changes in solution pH under physiological conditions. This reagent is found to be nontoxic, and the luminescence response could be used for imaging changes in endogenous pH induced by dexamethanose (DMT) in the endoplasmic reticulum.</p>1 aReddy, Upendar, G.1 aAnila, H., A.1 aAli, Firoj1 aTaye, Nandaraj1 aChattopadhyay, Samit1 aDas, Amitava  uhttps://library.ncl.res.in/content/fret-based-probe-monitoring-ph-changes-lipid-dense-region-hct116-cells-002406nas a2200217   4500008004100000022001400041245010000055210006900155260011000224300001400334490000600348520158500354100001601939700001501955700001901970700001801989700001602007700002502023700001702048856012302065       2015                        eng d  a2050-750X00aNew imaging reagents for lipid dense regions in live cells and the nucleus in fixed MCF-7 cells0 aNew imaging reagents for lipid dense regions in live cells and t  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcAUG  a7177-71850 v33 a<p>Two new uracil (U) and 5-flurouracil (5-FU) labeled ruthenium(II)-polypyridyl based cellular imaging reagents are reported. Confocal laser scanning microscopic images with live and paraformaldehyde (PFA) fixed MCF-7 cells are examined using these two low-cytotoxic reagents. Experimental results show that these two complexes, appropriately functionalized with U (1) and 5-FU (2), have specific affinity for the lipid dense regions like the endoplasmic reticulum, cell membrane, and cytoplasmic vacuoles in live MCF-7 cells, and dye internalization in these regions happened following an endocytosis pathway. Interestingly, these two complexes are found to be localized in the nucleus of the PFA fixed cells. For fixed cells, presumably the lipid layer disruption helped in the explicit localization of the complexes 1 and 2 in the cell nucleus through specific interaction with cellular DNA. Poor and non-specific internalization of an analogous model complex 3, without having a U or 5-FU moiety, reveals the definite influence of U or 5-FU as well as the role of lipophilicity of the respective complex 1 and 2 in the cellular internalization process. Apart from these, a large Stokes shift (similar to 160 nm) and an appreciably long lived (MLCT)-M-3 excited state (similar to 320 ns) in aq. buffer medium (pH 7.4) are other key features for complexes 1 and 2. Unlike the common nuclear DNA staining reagents like DAPI, these low-cytotoxic reagents are found to be highly stable towards photo-bleaching upon irradiation with 455 nm at the MLCT band for these complexes.</p>1 aRamu, Vadde1 aAli, Firoj1 aTaye, Nandaraj1 aGarai, Bikash1 aAlam, Aftab1 aChattopadhyay, Samit1 aDas, Amitava  uhttps://library.ncl.res.in/content/new-imaging-reagents-lipid-dense-regions-live-cells-and-nucleus-fixed-mcf-7-cells-001241nas a2200193   4500008004100000022001400041245011900055210006900174260011000243300001400353490000700367520044800374100002300822700001500845700001900860700002500879700001700904856012600921       2015                        eng d  a1359-734500aNew turn on Pd2+-specific fluorescence probe and its use as an imaging reagent for cellular uptake in Hct116 cells0 aNew turn on Pd2specific fluorescence probe and its use as an ima  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcJAN  a3649-36520 v513 a<p>A new coumarin-rhodamine conjugate is used as a specific probe for Pd2+ ions and this could even delineate Pd(II) from Pd(0) or Pd(IV) in aqueous buffer medium (pH similar to 7). Laser confocal microscopic studies reveal that efficient cellular internalization of this reagent helps in imaging the cellular uptake of Pd2+ as low as 0.1 ppm in Hct 116 cells. This reagent could even be used for estimation of Pd2+ in human urine samples.</p>1 aReddy, Upendar, G.1 aAli, Firoj1 aTaye, Nandaraj1 aChattopadhyay, Samit1 aDas, Amitava  uhttps://library.ncl.res.in/content/new-turn-pd2-specific-fluorescence-probe-and-its-use-imaging-reagent-cellular-uptake-001209nas a2200205   4500008004100000022001400041245015300055210006900208260011000277300001600387490000700403520034900410100001800759700002300777700001500800700001900815700002500834700001700859856012700876       2015                        eng d  a1359-734500aReagent for specific recognition of cysteine in aqueous buffer and in natural milk: imaging studies, enzymatic reaction and analysis of whey protein0 aReagent for specific recognition of cysteine in aqueous buffer a  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcAUG  a15592-155950 v513 a<p>We report a new chemodosimetric probe (L) for specific recognition of cysteine (Cys) in aqueous buffer and in whey protein isolated from fresh cow's milk. Using this reagent we could develop a luminescence-based methodology for estimation of Cys released from a commercially available Cys-supplement drug by aminoacylase-1 in live cells.</p>1 aAnila, H., A.1 aReddy, Upendar, G.1 aAli, Firoj1 aTaye, Nandaraj1 aChattopadhyay, Samit1 aDas, Amitava  uhttps://library.ncl.res.in/content/reagent-specific-recognition-cysteine-aqueous-buffer-and-natural-milk-imaging-studies-002773nas a2200241   4500008004100000022001400041245011700055210006900172260007100241300001600312490000800328520190000336100001502236700001702251700001902268700001902287700002202306700001502328700002302343700002502366700001702391856012302408       2015                        eng d  a1520-610600aSpecific reagent for Cr(III): imaging cellular uptake of Cr(III) in Hct116 cells and theoretical rationalization0 aSpecific reagent for CrIII imaging cellular uptake of CrIII in H  a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcOCT  a13018-130260 v1193 a<p>A new rhodamine-based reagent (L-1), trapped inside the micellar structure of biologically benign Triton-X 100, could be used for specific recognition of Cr(III) in aqueous buffer medium having physiological pH. This visible light excitable reagent on selective binding to Cr(III) resulted in a strong fluorescence turn-on response with a maximum at similar to 583 nm and tail of that luminescence band extended until 650 nm, an optical response that is desired for avoiding the cellular autofluorescence. Interference studies confirm that other metal ions do not interfere with the detection process of Cr(III) in aqueous buffer medium having pH 7.2. To examine the nature of binding of Cr(III) to L-1, various spectroscopic studies are performed with the model reagent L-1 which tend to support Cr(III)-eta(2)-olefin pi-interactions involving two olefin bonds in molecular probe L-1. Computational studies are also performed with another model reagent L-M to examine the possibility of such Cr(III)-eta(2)-olefin pi-interactions. Presumably, polar functional groups of the model reagent L-M upon coordination to the Cr(III) center effectively reduce the formal charge on the metal ion and this is further substantiated by results of the theoretical studies. This assembly is found to be cell membrane permeable and shows insignificant toxicity toward live colon cancer cells (Hct116). Confocal laser scanning microscopic studies further revealed that the reagent L-1 could be used as an imaging reagent for detection of cellular uptake of Cr(III) in pure aqueous buffer medium by Hct116 cells. Examples of a specific reagent for paramagnetic Cr(III) with luminescence ON response are scanty in the contemporary literature. This ligand design helped us in achieving the turn on response by utilizing the conversion from spirolactam to an acyclic xanthene form on coordination to Cr(III).</p>1 aAli, Firoj1 aSaha, Sukdeb1 aMaity, Arunava1 aTaye, Nandaraj1 aSi, Mrinal, Kanti1 aSuresh, E.1 aGanguly, Bishwajit1 aChattopadhyay, Samit1 aDas, Amitava  uhttps://library.ncl.res.in/content/specific-reagent-criii-imaging-cellular-uptake-criii-hct116-cells-and-theoretical-000686nas a2200181   4500008004100000245012500041210006900166260000800235300001600243490000700259100001800266700001500284700002100299700001900320700002500339700001700364856012300381       2016                        eng d00aCysteine-specific fluorescent switch for monitoring oxidative stress and quantification of aminoacylase-1 in blood serum0 aCysteinespecific fluorescent switch for monitoring oxidative str  cDEC  a12161-121680 v881 aAnila, H., A.1 aAli, Firoj1 aKushwaha, Shilpi1 aTaye, Nandaraj1 aChattopadhyay, Samit1 aDas, Amitava  uhttps://library.ncl.res.in/content/cysteine-specific-fluorescent-switch-monitoring-oxidative-stress-and-quantification03045nas a2200229   4500008004100000245017800041210006900219260000800288300001400296490000700310520219500317100001502512700001602527700001902543700002302562700002102585700001902606700002102625700002502646700001702671856012702688       2016                        eng d00aGSH Induced controlled release of levofloxacin from a purpose-built prodrug: luminescence response for probing the drug release in escherichia coli and staphylococcus aureus0 aGSH Induced controlled release of levofloxacin from a purposebui  cSEP  a2062-20700 v273 a<p>Fluoroquinolones are third-generation broad spectrum bactericidal antibiotics and work against both Gram-positive and Gram-negative bacteria. Levofloxacin (L), a fluoroquinolone, is widely used in anti-infective chemotherapy and treatment of urinary tract infection and pneumonia. The main pathogen for urinary tract infections is Escherichia colt, and Streptococcus pneumoniae is responsible for pneumonia, predominantly a lower respiratory tract infection. Poor permeability of L leads to the use of higher dose of this drug and excess drug in the outer cellular fluid leads to central nervous system (CNS) abnormality. One way to counter this is to improve the lipophilicity of the drug molecule, and accordingly, we have synthesized two new Levofloxacin derivatives, which participated in the spatiotemporal release of drug via disulfide bond cleavage induced by glutathione (GSH). Recent studies with Streptococcus mutants suggest that it is localized in epithelial lining fluid (ELF) of the normal lower respiratory tract and the effective [GSH] in ELF is similar to 430 mu M. E. coli typically cause urinary tract infections and the concentration of GSH in porcine bladder epithelium is reported as 0.6 mM for a healthy human. Thus, for the present study we have chosen two important bacteria (Gram + ve and Gram - ve), which are operational in regions having high extracellular GSH concentration. Interestingly, this supports our design of new lipophilic Levofloxacin based prodrugs, which released effective drug on reaction with GSH. Higher lipophilicity favored improved uptake of the prodrugs. Site specific release of the drug (L) could be achieved following a glutathione mediated biochemical transformation process through cleavage of a disulfide bond of these purpose-built prodrugs. Further, appropriate design helped us to demonstrate that it is possible also to control the kinetics of the drug release from respective prodrugs. Associated luminescence enhancement helps in probing the release of the drug from the prodrug in bacteria and helps in elucidating the mechanistic pathway of the transformation. Such an example is scarce in the contemporary literature.</p>1 aPal, Suman1 aRamu, Vadde1 aTaye, Nandaraj1 aMogare, Devraj, G.1 aYeware, Amar, M.1 aSarkar, Dhiman1 aReddy, Srinivasa1 aChattopadhyay, Samit1 aDas, Amitava  uhttps://library.ncl.res.in/content/gsh-induced-controlled-release-levofloxacin-purpose-built-prodrug-luminescence-response01330nas a2200205   4500008004100000022001400041245012700055210006900182260011000251300001400361490000700375520049600382100001500878700001800893700001900911700002300930700002500953700001700978856012900995       2016                        eng d  a1359-734500aSpecific receptor for hydrazine: mapping the in situ release of hydrazine in live cells and in an in vitro enzymatic assay0 aSpecific receptor for hydrazine mapping the in situ release of h  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcMAR  a6166-61690 v523 a<p>We report a new chemodosimetric reagent capable of detecting hydrazine in the presence of several other competing amine derivatives and ionic analytes of biological relevance. This reagent has been utilized for real time monitoring of in situ N2H4 release during the metabolism of a crucial tuberculosis drug, isoniazid, in live HepG2 cells. The fluorescence response of the reagent based on its specific reaction with N2H4 is used for developing an in vitro assay for aminoacylase-1.</p>1 aAli, Firoj1 aAnila, H., A.1 aTaye, Nandaraj1 aMogare, Devraj, G.1 aChattopadhyay, Samit1 aDas, Amitava  uhttps://library.ncl.res.in/content/specific-receptor-hydrazine-mapping-situ-release-hydrazine-live-cells-and-vitro-enzymatic02218nas a2200217   4500008004100000245009700041210006900138260000800207300001600215490000700231520147200238100002401710700002401734700001901758700001901777700002301796700002401819700002501843700001701868856011501885       2016                        eng d00aSwitch-on NIR probe for specific detection of Hg2+ ion in aqueous medium and in mitochondria0 aSwitchon NIR probe for specific detection of Hg2 ion in aqueous   cNOV  a12052-120600 v553 aA new 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY)-based probe molecule (L) is synthesized for specific binding to Hg2+ ion in physiological condition with an associated luminescence ON response in the near-IR region of the spectrum. Appropriate functionalization in the 5-position of each of two pyrrole moieties with styryl functionality in a BODIPY core helped us in achieving the extended conjugation and a facile intramolecular charge transfer transition with a narrow energy gap for frontier orbitals. This accounted for a poor emission quantum yield for the probe molecule L. Binding to. Hg2+ helped in interrupting the facile intramolecular charge transfer (ICT) process that was initially operational for L. This resulted in a hypsochromic shift of absorption band and a turn-on luminescence response with lambda(Em)(max) of 650 nm on specific binding to Hg2+. Observed spectral changes are rationalized based on quantum chemical calculations. Interestingly, this reagent is found to be localized preferentially in the mitochondria of the live human colon cancer (Hct116) cells. Mitochondria is one of the major targets for localization of He2+, which actually decreases the mitochondrial membrane potential and modifies various proteins having sulfudryl functionality(ies) to cause cell apoptosis. Considering these, ability of the present reagent to specifically recognize Hg2+] in the mitochondrial region of the live Hct116 cells has significance.1 aAgarwalla, Hrideshg1 aMahajan, Pankaj, S.1 aSahu, Debashis1 aTaye, Nandaraj1 aGanguly, Bishwajit1 aMhaske, Santosh, B.1 aChattopadhyay, Samit1 aDas, Amitava  uhttps://library.ncl.res.in/content/switch-nir-probe-specific-detection-hg2-ion-aqueous-medium-and-mitochondria02812nas a2200217   4500008004100000245016800041210006900209260000800278300001200286490000600298520200000304100001902304700002302323700001402346700002102360700001602381700002102397700002502418700001902443856013202462       2017                        eng d00aOxidant mediated one-step complete conversion of multi-walled carbon nanotubes to graphene quantum dots and their bioactivity against mammalian and bacterial cells0 aOxidant mediated onestep complete conversion of multiwalled carb  cJAN  a785-7960 v53 aIt is essential for any antibacterial agent (for clinical applications) that it should have high and selective toxicity towards bacterial cells only, and should not affect the human cells at the concentration used. Graphene quantum dots (GQDs) have emerged as a potential candidate for biomedical applications. However, a simple, low cost, safe, easy to execute, one-step synthesis of uniform and monodispersed GQDs with selective toxicity towards bacterial cells rather than mammalian cells is difficult to achieve. Herein, we have reported a one-step, low-cost, aqueous-phase, simple approach for the complete conversion of multi-walled carbon nanotubes into water-dispersible GQDs with an average size of similar to 3 nm using sodium bismuthate (NaBiO3) as a strong oxidant. The cyclic voltammetry and X-ray photoelectron spectroscopy results indicated that the as-synthesized GQDs suspension possess almost negligible amounts of metallic impurities. The cytotoxicity studies of GQDs against mammalian NIH 3T3 (mouse embryo fibroblast cells) and HEK 293T (human embryonic kidney cells) cells showed that the as-synthesized GQDs were non-cytotoxic up to the concentration of similar to 200 mu g mL(-1). The antimicrobial study shows that the synthesized GQDs have high and selective toxicity towards bacterial cells with a minimum inhibitory concentration of similar to 256 mu g mL(-1) for E. coli and B. subtilis and similar to 512 mu g mL(-1) for P. aeruginosa and S. aureus. The scanning electron microscopy and atomic force microscopy images show extensive cell damage via the perturbation of bacterial cell walls, which was consistent with the enhancement of reactive oxygen species production by almost two times in the bacterial cells upon incubation with similar to 256 mu g mL(-1) GQDs. Our study suggested that the as-synthesized GQDs can be used as a potential candidate for clinical applications as they possess high toxicity to bacterial cells and low toxicity to mammalian cells.1 aBiswas, Anupam1 aKhandelwal, Puneet1 aDas, Raja1 aSalunke, Gayatri1 aAlam, Aftab1 aGhorai, Suvankar1 aChattopadhyay, Samit1 aPoddar, Pankaj  uhttps://library.ncl.res.in/content/oxidant-mediated-one-step-complete-conversion-multi-walled-carbon-nanotubes-graphene-quantum02261nas a2200241   4500008004100000245014200041210006900183260000800252300001400260490000700274520140700281100001601688700001601704700001901720700002201739700002401761700002001785700002301805700002001828700002501848700001701873856012901890       2017                        eng d00aPhoto-induced cytotoxicity and anti-metastatic activity of ruthenium(II)-polypyridyl complexes functionalized with tyrosine or tryptophan0 aPhotoinduced cytotoxicity and antimetastatic activity of rutheni  cMAY  a6634-66440 v463 aThe synergistic effect of oxygen, light, and photosensitizer (PS) has found applications in medicine for the treatment of cancer through photodynamic therapy (PDT). Induction of apoptosis to cancerous cells will prevent tumor metastasis that spreads cancer cells to the neighboring organs/tissues. Herein, we report the two apoptotic Ru(II)-polypyridyl complexes that are functionalized with pendant amino acid moieties tyrosine (1) and tryptophan (2), respectively. These two water soluble complexes were found to interact strongly (K-a(1) = (1.18 +/- 0.28) x 10(5) M-1 and K-a(2) = (1.57 +/- 0.77) x 10(5) M-1) with CT-DNA. Isothermal titration calorimetry (ITC) studies revealed that these complexes bind to CT-DNA through an entropically driven process. Both the complexes showed photo-induced cytotoxicity and exhibit apoptotic activity under photo-irradiation conditions. The comet assay indicated that these complexes can damage cellular DNA, which is attributed to the significant build-up of O-1(2) level even on irradiation with low intensity light (10 J cm(-2), lambda(Range) 450-480 nm). This photoinduced DNA damage and apoptosis in A549 cells was induced by reactive oxygen species (ROS) and occurred through up-regulation of apoptotic marker caspase-3. Control experiments under dark conditions revealed an insignificant cytotoxicity towards these cells for two photosensitive molecules.1 aRamu, Vadde1 aAute, Sunil1 aTaye, Nandaraj1 aGuha, Rweetuparna1 aWalker, Michael, G.1 aMogare, Devaraj1 aParulekar, Apoorva1 aThomas, Jim, A.1 aChattopadhyay, Samit1 aDas, Amitava  uhttps://library.ncl.res.in/content/photo-induced-cytotoxicity-and-anti-metastatic-activity-rutheniumii-polypyridyl-complexes02421nas a2200157   4500008004100000245014700041210006900188260000800257520176200265100002302027700001602050700002302066700002502089700001902114856013002133       2018                        eng d00aRetention of anticancer activity of curcumin after conjugation with fluorescent gold quantum clusters: an in vitro and in vivo xenograft study0 aRetention of anticancer activity of curcumin after conjugation w  cMAY3 aGold nanoparticles (Au NPs) have been thoroughly investigated for anti-cancer therapy. However, their undesired high gold content remains a problem when injected into the body for drug delivery applications. In this report, we made an effort to conjugate the curcumin molecules on the surface of gold quantum clusters (Au QCs) by a novel in situ synthesis method which provides an alternative route to not only reduce the metallic content but also increase the water solubility of curcumin and the loading efficiency. Here, curcumin itself acts as a reducing and capping agent for the synthesis of Au QCs. The UV−vis absorption, fluorescence, transmission electron microscopy, and electrospray ionization mass spectrometry results confirmed the synthesis of fluorescent Au QCs. Curcumin-conjugated Au NPs (C-Au NPs) and glutathione (GSH)-conjugated Au QCs (GSH-Au QCs) were also synthesized to visualize the effect of particle size and the capping agent, respectively, on the cytotoxicity to normal and cancer cells. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that the curcumin-conjugated Au QCs (C-Au QCs) were less cytotoxic to normal cells while almost the same cytotoxic to cancer cells in comparison to curcumin itself, which indicates that curcumin preserves its anticancer property even after binding to the Au QCs. However, C-Au NPs and GSH-Au QCs did not show any cytotoxicity against the normal and cancer cells at the concentration used. The western blot assay indicated that C-Au QCs promote apoptosis in cancer cells. Further, the in vivo study on severe combined immunodeficiency mice showed that C-Au QCs also inhibited the tumor growth efficiently without showing significant toxicity to internal organs.1 aKhandelwal, Puneet1 aAlam, Aftab1 aChoksi, Arpankumar1 aChattopadhyay, Samit1 aPoddar, Pankaj  uhttps://library.ncl.res.in/content/retention-anticancer-activity-curcumin-after-conjugation-fluorescent-gold-quantum-clusters