01077nas a2200205 4500008004100000022001400041245017300055210006900228260011000297300001600407490000700423520019400430100002300624700001600647700001500663700001900678700002500697700001700722856013200739 2014 eng d a1359-734500aSpecific probe for Hg2+ to delineate even H+ in pure aqueous buffer/Hct116 colon cancer cells: Hg(II)-eta(2)-arene pi-interaction and a TBET-based fluorescence response0 aSpecific probe for Hg2 to delineate even H in pure aqueous buffe aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcSEP a14421-144240 v503 a

A new molecular probe that demonstrates a distinct TBET process, induced by the Hg(II)-eta(2)-arene pi-interaction, in pure aqueous medium with a large pseudo-Stokes shift of 200 nm.

1 aReddy, Upendar, G.1 aRamu, Vadde1 aRoy, Sovan1 aTaye, Nandaraj1 aChattopadhyay, Samit1 aDas, Amitava uhttps://library.ncl.res.in/content/specific-probe-hg2-delineate-even-h-pure-aqueous-bufferhct116-colon-cancer-cells-hgii-eta2-002773nas a2200241 4500008004100000022001400041245011700055210006900172260007100241300001600312490000800328520190000336100001502236700001702251700001902268700001902287700002202306700001502328700002302343700002502366700001702391856012302408 2015 eng d a1520-610600aSpecific reagent for Cr(III): imaging cellular uptake of Cr(III) in Hct116 cells and theoretical rationalization0 aSpecific reagent for CrIII imaging cellular uptake of CrIII in H a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcOCT a13018-130260 v1193 a

A new rhodamine-based reagent (L-1), trapped inside the micellar structure of biologically benign Triton-X 100, could be used for specific recognition of Cr(III) in aqueous buffer medium having physiological pH. This visible light excitable reagent on selective binding to Cr(III) resulted in a strong fluorescence turn-on response with a maximum at similar to 583 nm and tail of that luminescence band extended until 650 nm, an optical response that is desired for avoiding the cellular autofluorescence. Interference studies confirm that other metal ions do not interfere with the detection process of Cr(III) in aqueous buffer medium having pH 7.2. To examine the nature of binding of Cr(III) to L-1, various spectroscopic studies are performed with the model reagent L-1 which tend to support Cr(III)-eta(2)-olefin pi-interactions involving two olefin bonds in molecular probe L-1. Computational studies are also performed with another model reagent L-M to examine the possibility of such Cr(III)-eta(2)-olefin pi-interactions. Presumably, polar functional groups of the model reagent L-M upon coordination to the Cr(III) center effectively reduce the formal charge on the metal ion and this is further substantiated by results of the theoretical studies. This assembly is found to be cell membrane permeable and shows insignificant toxicity toward live colon cancer cells (Hct116). Confocal laser scanning microscopic studies further revealed that the reagent L-1 could be used as an imaging reagent for detection of cellular uptake of Cr(III) in pure aqueous buffer medium by Hct116 cells. Examples of a specific reagent for paramagnetic Cr(III) with luminescence ON response are scanty in the contemporary literature. This ligand design helped us in achieving the turn on response by utilizing the conversion from spirolactam to an acyclic xanthene form on coordination to Cr(III).

1 aAli, Firoj1 aSaha, Sukdeb1 aMaity, Arunava1 aTaye, Nandaraj1 aSi, Mrinal, Kanti1 aSuresh, E.1 aGanguly, Bishwajit1 aChattopadhyay, Samit1 aDas, Amitava uhttps://library.ncl.res.in/content/specific-reagent-criii-imaging-cellular-uptake-criii-hct116-cells-and-theoretical-001330nas a2200205 4500008004100000022001400041245012700055210006900182260011000251300001400361490000700375520049600382100001500878700001800893700001900911700002300930700002500953700001700978856012900995 2016 eng d a1359-734500aSpecific receptor for hydrazine: mapping the in situ release of hydrazine in live cells and in an in vitro enzymatic assay0 aSpecific receptor for hydrazine mapping the in situ release of h aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcMAR a6166-61690 v523 a

We report a new chemodosimetric reagent capable of detecting hydrazine in the presence of several other competing amine derivatives and ionic analytes of biological relevance. This reagent has been utilized for real time monitoring of in situ N2H4 release during the metabolism of a crucial tuberculosis drug, isoniazid, in live HepG2 cells. The fluorescence response of the reagent based on its specific reaction with N2H4 is used for developing an in vitro assay for aminoacylase-1.

1 aAli, Firoj1 aAnila, H., A.1 aTaye, Nandaraj1 aMogare, Devraj, G.1 aChattopadhyay, Samit1 aDas, Amitava uhttps://library.ncl.res.in/content/specific-receptor-hydrazine-mapping-situ-release-hydrazine-live-cells-and-vitro-enzymatic02218nas a2200217 4500008004100000245009700041210006900138260000800207300001600215490000700231520147200238100002401710700002401734700001901758700001901777700002301796700002401819700002501843700001701868856011501885 2016 eng d00aSwitch-on NIR probe for specific detection of Hg2+ ion in aqueous medium and in mitochondria0 aSwitchon NIR probe for specific detection of Hg2 ion in aqueous  cNOV a12052-120600 v553 aA new 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY)-based probe molecule (L) is synthesized for specific binding to Hg2+ ion in physiological condition with an associated luminescence ON response in the near-IR region of the spectrum. Appropriate functionalization in the 5-position of each of two pyrrole moieties with styryl functionality in a BODIPY core helped us in achieving the extended conjugation and a facile intramolecular charge transfer transition with a narrow energy gap for frontier orbitals. This accounted for a poor emission quantum yield for the probe molecule L. Binding to. Hg2+ helped in interrupting the facile intramolecular charge transfer (ICT) process that was initially operational for L. This resulted in a hypsochromic shift of absorption band and a turn-on luminescence response with lambda(Em)(max) of 650 nm on specific binding to Hg2+. Observed spectral changes are rationalized based on quantum chemical calculations. Interestingly, this reagent is found to be localized preferentially in the mitochondria of the live human colon cancer (Hct116) cells. Mitochondria is one of the major targets for localization of He2+, which actually decreases the mitochondrial membrane potential and modifies various proteins having sulfudryl functionality(ies) to cause cell apoptosis. Considering these, ability of the present reagent to specifically recognize Hg2+] in the mitochondrial region of the live Hct116 cells has significance.1 aAgarwalla, Hrideshg1 aMahajan, Pankaj, S.1 aSahu, Debashis1 aTaye, Nandaraj1 aGanguly, Bishwajit1 aMhaske, Santosh, B.1 aChattopadhyay, Samit1 aDas, Amitava uhttps://library.ncl.res.in/content/switch-nir-probe-specific-detection-hg2-ion-aqueous-medium-and-mitochondria