02605nas a2200253   4500008004100000022001400041245018200055210006900237260005700306300001200363490000700375520164900382653001602031653002202047653001602069653001202085653002902097100002402126700001402150700001302164700001602177700002602193856013202219       2013                        eng d  a0175-759800aCombinatorial approach of statistical optimization and mutagenesis for improved production of acidic phytase by Aspergillus niger NCIM 563 under submerged fermentation condition0 aCombinatorial approach of statistical optimization and mutagenes  a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcJAN  a673-6790 v973 a<p>Combination of statistical optimization and mutagenesis to isolate hypersecretory strains is studied to maximize phytase production from Aspergillus niger NCIM 563 under submerged fermentation. The overall results obtained show a remarkable 5.98-fold improvement in phytase production rates when compared to that using basal medium. Optimization of culture conditions from parent strain is studied first by the Plackett-Burman technique to evaluate the effects of 11 variables for phytase production. The results showed that glucose, MgSO4, KCl, incubation period, and MnSO4 are the most significant variables affecting enzyme production. Further optimization in these variables, using a central composite design technique, resulted in 3.74-fold increase in the yield of phytase production to 254,500 U/l when compared with the activity observed with basal media (68,000 U/l) in shake flask. Our experiments show that the phytase from A. niger NCIM 563 exhibits desirable activity in simulated gastric fluid conditions with low pH and also improved thermostability when compared to commercial phytase. The improved yield demonstrates the potential applicability of phytase enzyme as a source of phytase supplement for phosphorus nutrition and environmental protection in animal feed industry. Physical and chemical mutagenesis experiments were carried out in parallel to isolate hypersecretory mutants that could possibly further enhance the enzyme production. Using optimized media conditions of the parent strain, our results show that mutant strain A. niger NCIM 1359 increased the phytase activity by another 1.6-fold to 407,200 U/l.</p>10aAnimal feed10aAspergillus niger10aMutagenesis10aPhytase10aStatistical optimization1 aBhavsar, Kavita, P.1 aGujar, P.1 aShah, P.1 aKumar, Ravi1 aKhire, Jayant, Malhar  uhttps://library.ncl.res.in/content/combinatorial-approach-statistical-optimization-and-mutagenesis-improved-production-acidic-002127nas a2200265   4500008004100000022001400041245012200055210006900177260009900246300001200345490000800357520114100365653002501506653001401531653003701545653002701582653000901609100001901618700001701637700002401654700002201678700001601700700002101716856012401737       2015                        eng d  a0960-852400aConversion of dried aspergillus candidus mycelia grown on waste whey to biodiesel by in situ acid transesterification0 aConversion of dried aspergillus candidus mycelia grown on waste   aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcDEC  a502-5070 v1973 a<p>This study reports optimization of the transesterification reaction step on dried biomass of an oleaginous fungus Aspergillus candidus grown on agro-dairy waste, whey. Acid catalyzed transesterification was performed and variables affecting esterification, viz., catalyst methanol and chloroform concentrations, temperature, time, and biomass were investigated. Statistical optimization of the transesterification reaction using Plackett-Burman Design showed biomass to be the predominant factor with a 12.5-fold increase in total FAME from 25.6 to 320 mg. Studies indicate that the transesterification efficiency in terms of conversion is favored by employing lower biomass loadings. A. candidus exhibited FAME profiles containing desirable saturated (30.2%), monounsaturated (31.5%) and polyunsaturated methyl esters (38.3%). The predicted and experimentally determined biodiesel properties (density, kinematic viscosity, iodine value, cetane number, TAN, water content, total and free glycerol) were in accordance with international (ASTM D6751, EN 14214) and national (IS 15607) standards. (C) 2015 Published by Elsevier Ltd.</p>10aAspergillus candidus10aBiodiesel10aIn situ acid transesterification10aPlackett-Burman Design10aWhey1 aKakkad, Hardik1 aKhot, Mahesh1 aZinjarde, Smita, S.1 aRaviKumar, Ameeta1 aKumar, Ravi1 aKulkarni, B., D.  uhttps://library.ncl.res.in/content/conversion-dried-aspergillus-candidus-mycelia-grown-waste-whey-biodiesel-situ-acid-0