02127nas a2200265 4500008004100000022001400041245012200055210006900177260009900246300001200345490000800357520114100365653002501506653001401531653003701545653002701582653000901609100001901618700001701637700002401654700002201678700001601700700002101716856012401737 2015 eng d a0960-852400aConversion of dried aspergillus candidus mycelia grown on waste whey to biodiesel by in situ acid transesterification0 aConversion of dried aspergillus candidus mycelia grown on waste  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcDEC a502-5070 v1973 a

This study reports optimization of the transesterification reaction step on dried biomass of an oleaginous fungus Aspergillus candidus grown on agro-dairy waste, whey. Acid catalyzed transesterification was performed and variables affecting esterification, viz., catalyst methanol and chloroform concentrations, temperature, time, and biomass were investigated. Statistical optimization of the transesterification reaction using Plackett-Burman Design showed biomass to be the predominant factor with a 12.5-fold increase in total FAME from 25.6 to 320 mg. Studies indicate that the transesterification efficiency in terms of conversion is favored by employing lower biomass loadings. A. candidus exhibited FAME profiles containing desirable saturated (30.2%), monounsaturated (31.5%) and polyunsaturated methyl esters (38.3%). The predicted and experimentally determined biodiesel properties (density, kinematic viscosity, iodine value, cetane number, TAN, water content, total and free glycerol) were in accordance with international (ASTM D6751, EN 14214) and national (IS 15607) standards. (C) 2015 Published by Elsevier Ltd.

10aAspergillus candidus10aBiodiesel10aIn situ acid transesterification10aPlackett-Burman Design10aWhey1 aKakkad, Hardik1 aKhot, Mahesh1 aZinjarde, Smita, S.1 aRaviKumar, Ameeta1 aKumar, Ravi1 aKulkarni, B., D. uhttps://library.ncl.res.in/content/conversion-dried-aspergillus-candidus-mycelia-grown-waste-whey-biodiesel-situ-acid-002462nas a2200277 4500008004100000022001400041245015600055210006900211260000900280300001200289490000800301520151800309653001401827653000901841653003201850653001401882653002201896653002401918100001701942700002001959700002001979700001601999700002102015700002202036856012602058 2018 eng d a0360-544200aOptimization of the in situ transesterification step for biodiesel production using biomass of Yarrowia lipolytica NCIM 3589 grown on waste cooking oil0 aOptimization of the in situ transesterification step for biodies cJAN  a944-9520 v1423 a

The in situ (one-step) acid-catalyzed transesterification step for conversion to biodiesel of biomass from oleaginous yeast Yarrowia lipolytica grown on waste cooking oil (WCO) is studied. The process yield of biodiesel was optimized by investigating effects of various parameters, namely, biomass, methanol, chloroform, catalyst, temperature, time and sonication. A Plackett-Burman statistical design of experiments revealed that biomass is the most significant factor influencing biodiesel (FAME, fatty acid methyl ester) production. Subsequently, a one variable design (OVD) of experiments for increased biomass loadings showed higher yields of FAME with no additional requirement of reactants, solvents or special equipment. The biomass grown on WCO had a lipid productivity of 0.042 g L-1 h(-1) and 4 g of this loading gave a high FAME yield of 0.88 gin 8 hat 50 degrees C with methanol: chloroform (10:1) and acid catalyst (0.2 M H2SO4,1.0 ml g(-1)). The FAME profile had desirable amounts of saturated (32.81%), monounsaturated (36.41%), polyunsaturated (30.59%) methyl esters. The predicted and experimentally determined physico-chemical properties of FAME were found in accordance with specified international standards. Thus, the direct one-pot in situ transesterification reaction using Y. lipolytica biomass grown on WCO provides a high yield of biodiesel with potential applicability while simultaneously addressing the management of this pollutant. (C) 2017 Elsevier Ltd. All rights reserved.

10aBiodiesel10aFAME10aIn situ transesterification10aOne -step10aWaste cooking oil10aYarrowia lipolytica1 aKatre, Gouri1 aRaskar, Shubham1 aZinjarde, Smita1 aKumar, Ravi1 aKulkarni, B., D.1 aRaviKumar, Ameeta uhttps://library.ncl.res.in/content/optimization-situ-transesterification-step-biodiesel-production-using-biomass-yarrowia02517nas a2200241 4500008004100000022001400041245011500055210006900170260000800239300000700247490000700254520170100261653002401962653001401986653003202000653001102032653002902043653001602072100002002088700001602108700002202124856012902146 2025 eng d a2191-085500aAspergillus terreus variant TB21 wet biomass optimized by in-situ transesterification for biodiesel production0 aAspergillus terreus variant TB21 wet biomass optimized by insitu cFEB a230 v153 a

The oleaginous fungus, Aspergillus terreus when subjected to random chemical mutagenesis led to isolation of TB21 variant with improved lipid content (78.1%) as compared to wild type (49.8%). The fungal wet biomass grown on sugarcane bagasse hydrolysate (SCBH) was subjected to one-step in-situ (direct) acid transesterification to optimize its conversion to biodiesel using a 2-level factorial statistical design of experiments. The process optimization revealed that wet biomass and methanol were the most significant factors and in a short reaction time period of 5 min with low methanol: wet biomass ratio (10:1) influenced FAME production Statistical optimization studies showed that TB21 exhibited a higher FAME content of 76.5 and 38.1% (w/w) from wet and dry biomass, respectively when compared to wild type (48.1 and 24.5%). FAME productivity (0.55-1 h-1) and a yield (66 gL-1) were achieved when TB21 was grown on SCBH for 120 h at 30 degrees C. The FAME profile from the wet biomass of TB21 grown on SCBH had desirable amounts of saturated (77.7%), monounsaturated (7.2%), and polyunsaturated (2.4%) methyl esters. Physico-chemical properties of TB21-derived biodiesel were determined, namely, density(0.88 g cm-3), kinematic viscosity (4.1 mm s-2), iodine value (96.82), cetane number (55.31), free fatty acid content (0.15%), total acid number (0.3 NaOH mg g-1), meeting international (ASTM D6751, EN 14214) and Indian (IS 15607) standards. Thus, the direct one-pot in situ transesterification reaction using wet biomass of variant TB21 strain showed improved production and quality of biodiesel with potential large scale application using the low-cost substrate (SCBH).

10aAspergillus Terreus10aBiodiesel10aIn situ transesterification10aMutant10aStatistical optimization10aWet biomass1 aBed, Rashmi, K.1 aKumar, Ravi1 aRaviKumar, Ameeta uhttps://library.ncl.res.in/content/aspergillus-terreus-variant-tb21-wet-biomass-optimized-situ-transesterification-biodiesel