01978nas a2200289   4500008004100000022001400041245011000055210006900165260009500234300001200329490000600341520094400347653001401291653002101305653001601326653001101342653002301353653001801376100002501394700001601419700002601435700002901461700002601490700001701516700002601533856012901559       2007                        eng d  a1550-703300aCdS quantum dots: enzyme mediated in vitro synthesis, characterization and conjugation with plant lectins0 aCdS quantum dots enzyme mediated in vitro synthesis characteriza  a25650 NORTH LEWIS WAY, STEVENSON RANCH, CA 91381-1439 USAbAMER SCIENTIFIC PUBLISHERScDEC  a406-4130 v33 a<p>This paper presents the in vitro synthesis of CdS quantum dot nanoparticles using sulphite reductase and capping peptide in the presence of a co-factor alpha-NADPH, their characterization and conjugation with plant lectins, Jacalin and Chick pea lectin. Incubation of sulphite reductase with aqueous CdCl2, Na2SO3, and capping peptide in the presence of a-NADPH, which acts as a co-factor, resulted in the formation of CdS nanoparticles of dimensions 5-20 nm. These CdS nanoparticles were conjugated with plant lectins by EDAC mediated coupling. These observations are of significance, as it will help in the development of eco-friendly process for the formation of CdS nanoparticles and their application as fluorescent biolabels. Moreover the above studies will also help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation.</p>10abio-label10aCdS quantum dots10aconjugation10aEnzyme10ain vitro synthesis10aNanoparticles1 aAnsary, Abu, Ayoobul1 aKumar, Anil1 aKrishnasastry, M., V.1 aAbyaneh, Majid, Kazemian1 aKulkarni, Sulabha, K.1 aAhmad, Absar1 aKhan, Mohammad, Islam  uhttps://library.ncl.res.in/content/cds-quantum-dots-enzyme-mediated-vitro-synthesis-characterization-and-conjugation-plant-002255nas a2200265   4500008004100000022001400041245013400055210006900189260009500258300001200353490000800365520121200373653002101585653002401606653003501630653001901665653002301684100002501707700002401732700002801756700002501784700002601809700003001835856012401865       2011                        eng d  a0003-269700aCarbon nanotube-modified sodium dodecyl sulfate-polyacrylamide gel electrophoresis for molecular weight determination of proteins0 aCarbon nanotubemodified sodium dodecyl sulfatepolyacrylamide gel  a525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USAbACADEMIC PRESS INC ELSEVIER SCIENCEcFEB  a230-2350 v4093 a<p>The effect of incorporating carbon nanotubes (CNTs) in the gel matrix on the electrophoretic mobility of proteins based on their molecular weight differences was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). More specifically, a reduction in standard deviation in the molecular weight calibration plots by 55% in the case of multiwalled carbon nanotubes (MWCNTs) and by 34% in the case of single-walled carbon nanotubes (SWCNTs) compared with that of pristine polyacrylamide gels was achieved after incorporating an insignificant amount of functionalized CNTs into the gel matrix. A mechanism based on a more uniform pore size distribution in CNT modified polyacrylamide gel matrix is proposed. Furthermore, the impact of SWCNTs and MWCNTs on the mobility of proteins in different molecular weight regimes at a given acrylamide concentration offers a tunable gel matrix in terms of the selection of molecular weight ranges of proteins. The robustness and excellent reproducibility of the CNT-PAGE protocol are expected to have a significant impact on the molecular weight determination of newly isolated proteins. (C) 2010 Elsevier Inc. All rights reserved.</p>10aCarbon nanotubes10aGel electrophoresis10aMolecular weight determination10aPolyacrylamide10aProtein separation1 aParthasarathy, Meera1 aDebgupta, Joyashish1 aKakade, Bhalchandra, A.1 aAnsary, Abu, Ayoobul1 aKhan, Mohammad, Islam1 aPillai, Vijayamohanan, K.  uhttps://library.ncl.res.in/content/carbon-nanotube-modified-sodium-dodecyl-sulfate-polyacrylamide-gel-electrophoresis-0