02664nas a2200301   4500008004100000022001400041245023700055210006900292260009500361300001000456490000700466520151000473653001101983653001601994653001702010653001002027653001402037653002002051653002202071653002702093653002402120100001602144700001502160700001702175700002802192700001602220856012602236       2006                        eng d  a0048-357500aFlufenoxuron, an acylurea insect growth regulator, alters development of Tribolium castaneum (Herbst) (Coleoptera :tenebrionidae) by modulating levels of chitin, soluble protein content, and HSP70 and p34(cdc2) in the larval tissues0 aFlufenoxuron an acylurea insect growth regulator alters developm  a525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USAbACADEMIC PRESS INC ELSEVIER SCIENCEcJUN  a84-900 v853 a<p>The effect of sublethal concentrations, 0.00141% (LC20), 0.00251% (LC30), and 0.0036% (LC40) of a dispersible formulation of an acylurea insect growth regulator, flufenoxuron (Cascade) on certain biochemical parameters in the larvae of Tribolium castaneum was investigated. When neonates were fed on diet treated with sublethal concentrations for 24 It, it was observed that at all concentrations tested, there was a significant reduction in chitin content on the 15th day of development. Total soluble protein content at LC20 and LC30 decreased with increasing age of the larvae. At LC20 and LC40 concentrations there was a progressive increase in the protein: chitin ratio as a function of increase in age of the larvae. SDS-PAGE analysis of the larval tissue extracts indicated gross quantitative changes in some of the protein bands (MW 50-97 kDa). Western blot analysis revealed significant increase in the level of HSP70 in the extracts of larvae fed on LC30 treated diet, on the 7th and 10th day of development in the decreasing order. Conversely, a significant decrease in the hyper-phosphorylated form of p34(cdc2) kinase due to flufenoxuron treatment indicating modulation of cell cycle regulation was observed. Thus, sublethal concentrations of flufenoxuron alter expression of developmentally regulated proteins, HSP70 and p34(cdc2) and chitin formation in a stage-specific manner thereby resulting developmental abnormalities in T castaneum. (c) 2005 Elsevier Inc. All rights reserved.</p>10achitin10aDevelopment10aflufenoxuron10aHSP7010ap34(cdc2)10aphosphotyrosine10asublethal effects10atotal soluble proteins10aTribolium castaneum1 aSalokhe, S.1 aSarkar, A.1 aKulkarni, A.1 aMukherjee, Samindra, N.1 aPal, J., K.  uhttps://library.ncl.res.in/content/flufenoxuron-acylurea-insect-growth-regulator-alters-development-tribolium-castaneum-0