02410nas a2200337   4500008004100000022001400041245011600055210006900171260009200240300001200332490000700344520123200351653002701583653002301610653001401633653001601647653002501663653001101688653002701699653001301726100002801739700002201767700002101789700002001810700002401830700002301854700002201877700002001899700002601919856012701945       2008                        eng d  a0958-315700aComparison of Metarhizium isolates for biocontrol of helicoverpa armigera (Lepidoptera : Noctuidae) in chickpea0 aComparison of Metarhizium isolates for biocontrol of helicoverpa  a4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLANDbTAYLOR & FRANCIS LTDcFEB  a809-8280 v183 a<p>Metarhizium isolates from soil (53) and insect hosts (10) were evaluated for extracellular production of cuticle degrading enzyme (CDE) activities such as chitinase, chitin deacetylase (CDA), chitosanase, protease and lipase. Regression analysis demonstrated the relation of CDE activities with Helicoverpa armigera mortality. On basis of this relation, ten isolates were selected for further evaluation. Subsequently, based on LT(50) of the 10 isolates towards H. armigera, five isolates were selected. Out of these five isolates, three were selected on the basis of higher conidia production (60-75 g/kg rice), faster sedimentation time (ST(50)) (2.3-2.65 h in 0.1% (w/v) Tween 80) and lower LC(50) (1.4- 5.7 x 10(3) conidia/mL) against H. armigera. Finally, three Metarhizium isolates were selected for the molecular fingerprinting using ITS sequencing and RAPD patterning. All three isolates, M34412, M34311 and M81123, showed comparable RAPD patterns with a 935G primer. These were further evaluated for their field performance against H. armigera in a chickpea crop. The percent efficacies with the three Metarhizium isolates were from 65 to 72%, which was comparable to the chemical insecticide, endosulfan (74%).</p>10aappressorium formation10achitin deacetylase10achitinase10achitosanase10aHelicoverpa armigera10aLipase10aMetarhizium anisopliae10aProtease1 aKulkarni, Shuklangi, A.1 aGhormade, Vandana1 aKulkarni, Girish1 aKapoor, Manisha1 aChavan, Santosh, B.1 aRajendran, Armugam1 aPatil, Sanjay, K.1 aShouche, Yogesh1 aDeshpande, Mukund, V.  uhttps://library.ncl.res.in/content/comparison-metarhizium-isolates-biocontrol-helicoverpa-armigera-lepidoptera-noctuidae-0