01139nas a2200157 4500008004100000245013700041210006900178260000800247490000600255520049900261100002500760700002000785700002600805700002300831856012700854 2016 eng d00aInvestigation of microbial diversity in geothermal hot springs in Unkeshwar, India, based on 16S rRNA amplicon metagenome sequencing0 aInvestigation of microbial diversity in geothermal hot springs i cFEB0 v43 a

Microbial diversity in geothermal waters of the Unkeshwar hot springs in Maharashtra, India, was studied using 16S rRNA amplicon metagenomic sequencing. Taxonomic analysis revealed the presence of Bacteroidetes , Proteobacteria , Cyanobacteria , Actinobacteria , Archeae , and OD1 phyla. Metabolic function prediction analysis indicated a battery of biological information systems indicating rich and novel microbial diversity, with potential biotechnological applications in this niche.

1 aMehetre, Gajanan, T.1 aParanjpe, Aditi1 aDastager, Syed, Gulam1 aDharne, Mahesh, S. uhttps://library.ncl.res.in/content/investigation-microbial-diversity-geothermal-hot-springs-unkeshwar-india-based-16s-rrna02587nas a2200301 4500008004100000022001400041245014900055210006900204260000800273300001200281490000800293520153000301653001301831653002901844653001401873653002901887100001801916700002201934700001401956700002001970700002001990700002702010700002602037700002902063700002402092700003002116856013902146 2017 eng d a0723-202000aInvestigation into the taxonomy of “ B. aminovorans” and its reclassification to the genus Domibacillus as Domibacillus aminovorans sp. nov.0 aInvestigation into the taxonomy of B aminovorans and its reclass cAUG a458-4670 v40 3 aThe species "Bacillus aminovorans" was categorized as species Incertae Sedis meaning taxa with uncertain taxonomic position because of insufficient description of phenotypic properties and lack of understanding of its phylogenetic relationship (Claus and Berkeley [5]). In this study two strains "B. aminovorans" DSM 1314T and DSM 4337 were phenotypically and phylogenomically analyzed. The strains are Gram-staining-positive, spore forming rods and unable to utilize/ferment most of the sugars tested except glucose. The major fatty acids are anteiso-C15:0 and iso-C15:0. The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol and an aminophosphoglycolipid. The cell wall peptidoglycan is of A1γ type with meso-Dpm as the diamino acid. The menaquinone type present is MK-6. The G+C content of the genomic DNA of the type strain is 40.8mol%. These characteristics were found to be in line with description of the genus Domibacillus. Moreover phylogenetic analysis based on 16S rRNA gene sequence retrieved "B. aminovorans" within the genus Domibacillus with D. antri XD 80T as the closest relative. Additionally genome sequencing of the strains and their comparison with whole genome sequences of other Domibacillus spp. confirmed their distinctiveness and separate species status within the genus based on parameters of genome to genome distance calculator (GGDC) and average nucleotide identity (ANI) values. Therefore a novel species Domibacillus aminovorans sp. nov. (DSM 1314T =LMG 16796T) is proposed.10a16S rRNA10aDomibacillus aminovorans10aMALDI-TOF10a``Bacillus aminovorans''1 aVerma, Ashish1 aOjha, Anup, Kumar1 aPal, Yash1 aKumari, Parveen1 aSchumann, Peter1 aGruber-Vodicka, Harald1 aDastager, Syed, Gulam1 aNatarajan, Ramesh, Kumar1 aMayilraj, Shanmugam1 aKrishnamurthi, Srinivasan uhttps://library.ncl.res.in/content/investigation-taxonomy-%E2%80%9C-b-aminovorans%E2%80%9D-and-its-reclassification-genus-domibacillus04290nas a2200253 4500008004100000245013100041210006900172260000800241490000700249520336900256100002203625700002803647700002003675700002403695700003003719700002003749700001803769700002603787700002303813700001303836700002803849700002703877856013203904 2024 eng d00aIntegrated genomic and functional analysis of Streptomyces sp. UP1A-1 for bacterial wilt control and solanaceae yield increase0 aIntegrated genomic and functional analysis of Streptomyces sp UP cDEC0 v373 a

Ralstonia solanacearum is one of the most destructive soil-borne pathogen, causing bacterial wilt to the solanaceae vegetables. Streptomyces sp. UP1A-1 isolated from healthy solanaceae rhizosphere soil, exhibited the lowest disease incidence and increased fruit yield of solanaceae vegetables. However, the genomic and functional properties of UP1A-1 are unclear. Therefore, we conducted the present study to elucidate the genomic characteristics of UP1A-1 by whole genome sequencing. The results indicate that the genome of Streptomyces sp. UP1A-1 consists of 8,252,902 bp and contains 72.42 % G + C. We identified the genes that confer plant growth promoting (PGP) function, which include those involved in siderophore production, indole-3-acetic acid biosynthesis, phosphate solubilization, nitrogen metabolism, and potassium metabolism. We also identified several other genes, such as chitinase, peroxidase, superoxide dismutase, catalase, proline biosynthesis, and glucose dehydrogenase, which are believed to be involved in the control of wilt disease. These genes revealed that the strain UP1A-1 has physiologically adapted to varied environmental conditions and could potentially control both abiotic and biotic stresses.

1 aKaari, Manigundan1 aManikkam, Radhakrishnan1 aJoseph, Jerrine1 aKrishnan, Sakthivel1 aAnnamalai, Kishore, Kumar1 aKhan, Abujunaid1 aRajput, Vinay1 aDastager, Syed, Gulam1 aDharne, Mahesh, S.1 aUmar, Md1 aVenugopal, Gopikrishnan1 aAlexander, Balamurugan uhttps://library.ncl.res.in/content/integrated-genomic-and-functional-analysis-streptomyces-sp-up1a-1-bacterial-wilt-control-and