02281nas a2200229   4500008004100000022001400041245013300055210006900188260011000257300001400367490000700381520133800388653002501726653001501751653004201766653001701808100002301825700003001848700002601878700001801904856012901922       2014                        eng d  a0019-518900aAnti-fungal potentials of extracellular metabolites of western ghats isolated streptomyces sp NII 1006 against moulds and yeasts0 aAntifungal potentials of extracellular metabolites of western gh  aDR K S KRISHNAN MARG, PUSA CAMPUS, NEW DELHI 110 012, INDIAbNATL INST SCIENCE COMMUNICATION-NISCAIRcNOV  a1138-11460 v523 a<p>Realization of hazardious effects of chemical fungicides has led to an interest in the usage of biocontrol agents. The present study, therefore, evaluates the biocontrol efficacy of Western Ghats (India) soil bacterial isolates. A potential strain NII 1006 was evaluated for its antagonistic property against a diverse range of moulds and yeasts. The strain was characterized morphologically, biochemically and molecularly, which revealed the isolate belonged to Streptomyces genus. Organic solvent extracts of NII 1006 culture filtrates inhibited the growth of the test pathogens indicating that growth suppression was due to extracellular anti-fungal metabolites present in the culture filtrates. The strain produced extracellular chitinase enzyme in addition to some stable partially purified anti-fungal compounds. Morphological changes such as hyphae degradation into debris and abnormal shapes were observed in test fungi and yeast grown on potato dextrose broth that contained the NII 1006 culture filtrate. The cell free supernatant has a tolerance to wide range of pH, temperature and enzymes such as lipase and protease. The biocontrol potential of NII 1006 strain may be correlated significantly with their ability to produce antibiotics as well as extracellular hydrolytic enzymes particularly chitinolytic enzyme.</p>10aAnti-fungal activity10aBiocontrol10aExtracellular anti-fungal metabolites10aStreptomyces1 aJayamurthy, Himani1 aSajna, Kuttavan, Valappil1 aDastager, Syed, Gulam1 aPandey, Ashok  uhttps://library.ncl.res.in/content/anti-fungal-potentials-extracellular-metabolites-western-ghats-isolated-streptomyces-sp-002290nas a2200253   4500008004100000022001400041245012700055210006900182260011000251300001400361490000700375520131500382653001701697653002301714653002101737653001701758653001501775100002701790700002401817700002001841700002601861700002001887856012901907       2014                        eng d  a0019-518900aDevelopment of bioconjugate from streptomyces tyrosinase and gold nanoparticles for rapid detection of phenol constituents0 aDevelopment of bioconjugate from streptomyces tyrosinase and gol  aDR K S KRISHNAN MARG, PUSA CAMPUS, NEW DELHI 110 012, INDIAbNATL INST SCIENCE COMMUNICATION-NISCAIRcNOV  a1071-10810 v523 a<p>Most of the phenol compounds are toxic and have been considered as hazardous pollutants. Several physicochemical and biological methods are available to detect and monitor the phenol pollutants in water and soil. In the present study, phenol constituents of winery, paper and plastic industrial effluents were successfully detected employing tyrosinase-gold nanoparticles bioconjugate. The synthesis of extracellular tyrosinase and gold nanoparticles was achieved by a single isolate of Streptomyces sp. DBZ-39. Enhanced production (369.41 IU) of tyrosinase was produced in submerged bioprocess employing response surface method with central composite design. Extracellular gold nanoparticles synthesized (12-18 nm) by Streptomyces sp. DBZ-39 were characterized with TEM, EDAX and FTIR analysis. A rapid detection (within 10 min) of phenol constituents from winery effluents was achieved by bioconjugate, when compared to tyrosinases and gold nanoparticles independently. Streptomyces tyrosinase could exhibit relatively a better performance than commercially available mushroom tyrosinase in the detection of phenol constituents. Winery effluent has shown much higher content (0.98 O.D) of phenol constituents than paper and plastic effluents based on the intensity of color and U.V absorption spectra.</p>10aBioconjugate10agold nanoparticles10aPhenol detection10aStreptomyces10aTyrosinase1 aZainab, Mazhari, Bi Bi1 aMadhusudhan, D., N.1 aRaghavendra, H.1 aDastager, Syed, Gulam1 aDayanand, Agsar  uhttps://library.ncl.res.in/content/development-bioconjugate-streptomyces-tyrosinase-and-gold-nanoparticles-rapid-detection-0