02151nas a2200265   4500008004100000022001400041245013600055210006900191260010200260300001000362490000800372520114400380653002401524653001101548653001501559653002501574653001701599100002401616700002501640700001601665700002601681700002801707700002201735856012801757       2014                        eng d  a0011-389100aCulturable bacterial phylogeny from a shallow water hydrothermal vent of espalamaca (Faial, Azores) reveals a variety of novel taxa0 aCulturable bacterial phylogeny from a shallow water hydrothermal  aC V RAMAN AVENUE, SADASHIVANAGAR, P B \#8005, BANGALORE 560 080, INDIAbINDIAN ACAD SCIENCEScJAN  a58-690 v1063 a<p>Phylogenetic diversity of the 16S rRNA gene associated with the domain bacteria was examined at the level of operational taxonomic units (OTUs) using the rarefaction analysis from a newly identified shallow water hydrothermal vent, Espalamaca in the Azorean Island Faial (Horta), North Atlantic Ocean. Diluted sea water nutrient agar amended with metals manganese, lead, iron and element sulphur, could help in retrieving highest OTUs (95) from the vent and 39 OTUs from nonvent. Molecular tools implemented on bacterial census indicated the occupancy of gamma-Proteobacteria by 55.78%, alpha-Proteobacteria by 21.05% and 12.63% of Bacteroidetes in the total population. Occurrence of novel species maximized with alpha-Proteobacteria (11/20) followed by Bacteroidetes (5/12) signified the necessity of studying these groups to strengthen the biodiversity database. Shannon index (H') and the Chao I richness estimator illustrated a strong bacterial community in the venting area. The current study confers many bacterial genera which were not reported earlier in any of the shallow water vents and adds 33 new taxa to the database.</p>10aBacterial phylogeny10ametals10anovel taxa10ararefaction analysis10ashallow vent1 aRajasabapathy, Raju1 aMohandass, Chellandi1 aColaco, Ana1 aDastager, Syed, Gulam1 aSantos, Ricardo, Serrao1 aMeena, Ram, Murti  uhttps://library.ncl.res.in/content/culturable-bacterial-phylogeny-shallow-water-hydrothermal-vent-espalamaca-faial-azores-002579nas a2200265   4500008004100000022001400041245014800055210006900203260007400272300001400346490000800360520159000368653001101958653003701969653002202006653002402028653001302052100002402065700002502089700002602114700001402140700001602154700001602170856012702186       2015                        eng d  a0003-607200aCitreicella manganoxidans sp nov., a novel manganese oxidizing bacterium isolated from a shallow water hydrothermal vent in Espalamaca (Azores)0 aCitreicella manganoxidans sp nov a novel manganese oxidizing bac  aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcDEC  a1433-14390 v1083 a<p>A Gram-stain negative, non-motile, non-spore forming, aerobic and rod or narrow lemon-shaped bacterial strain, VSW210(T), was isolated from surface seawater in a shallow water hydrothermal vent region in Espalamaca (Azores). Strain VSW210(T) was found to grow optimally at 30 A degrees C, at pH 7 and in the presence of 2-6 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain VSW210(T) clusters with the type strain Citreicella marina CK-I3-6(T) (sequence similarity value of 99.6 %), but DNA-DNA hybridization showed DNA-DNA relatedness between the strain VSW210(T) and C. marina CK-I3-6(T) to be 55.8 +/- A 3.2 %. The DNA G+C content of strain VSW210(T) was determined to be 67.4 mol%. The cellular fatty acid profiles of strain VSW210(T) was found to contain C-18:1 omega 7c (80.1 %) and C-16:0 (9.2 %). The major polar lipids in strain VSW210(T) were identified as phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified phospholipid. Strain VSW210(T) was found to be able to oxidize soluble Mn(II) to insoluble MnO2, which was confirmed with LBB staining. Differential phenotypic properties and genetic uniqueness revealed that this strain VSW210(T) is distinguishable from other species of the genus Citreicella. On the basis of the data presented, strain VSW210(T) is considered to represent a novel species of the genus Citreicella, for which the name Citreicella manganoxidans sp. nov. is proposed. The type strain is VSW210(T) (=KCTC 32497(T) = MCC 2286(T)).</p>10aAzores10aCitreicella manganoxidans sp nov10aHydrothermal vent10aPolyphasic taxonomy10aSeawater1 aRajasabapathy, Raju1 aMohandass, Chellandi1 aDastager, Syed, Gulam1 aLiu, Qing1 aLi, Wen-Jun1 aColaco, Ana  uhttps://library.ncl.res.in/content/citreicella-manganoxidans-sp-nov-novel-manganese-oxidizing-bacterium-isolated-shallow-0