02379nas a2200253   4500008004100000022001400041245012000055210006900175260009900244300001400343490000700357520141500364653001401779653001901793653005001812653002401862653002001886653002801906100001301934700002001947700001601967700001301983856012901996       2008                        eng d  a0142-961200aProtection of oligo deoxynucleotides against nuclease degradation through association with self-assembling peptides0 aProtection of oligo deoxynucleotides against nuclease degradatio  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcMAR  a1099-11080 v293 a<p>Aggregates of the self-assembling peptide EAK16II or EAK16IV and oligodeoxynucleotides (ODNs) were prepared, and their stability upon diluting the solution was investigated by UV-vis spectroscopy. The aggregates prepared at pH 4 and pH 7 did not dissociate after the solution was diluted 5- and 10-fold. The resistance against Escherichia coli exonuclease I of the ODN located in the EAK-ODN aggregates was studied by fluorescence resonance energy transfer (FRET) after the ODN had aggregated with EAK 16II or EAK 16IV at pH 4 or pH 7. The effect that the peptide sequence, peptide concentration, pH, and centrifugation had on protecting the aggregated ODN against nuclease degradation was investigated. Significant nuclease resistance was obtained after the EAK-ODN aggregates had been prepared at pH 4, with an EAK16IV concentration greater than a threshold value, and ensuring that the solution was not centrifuged immediately after sample preparation. Centrifuging the EAK16IV-ODN solution immediately after sample preparation resulted in the loss of this nuclease protection. However, if the solution of EAK-ODN aggregates was centrifuged 24h after sample preparation, the nuclease protection afforded by the EAK16IV-ODN aggregates to the ODN was maintained even after being subject to a 10-fold dilution and up to 4 rounds of centrifugation over 4 days. (c) 2007 Elsevier Ltd. All rights reserved.</p>10aaggregate10acentrifugation10aFluorescence resonance energy transfer (FRET)10anuclease resistance10aoligonucleotide10aself-assembling peptide1 aWang, M.1 aAdikane, H., V.1 aDuhamel, J.1 aChen, P.  uhttps://library.ncl.res.in/content/protection-oligo-deoxynucleotides-against-nuclease-degradation-through-association-self-0