01941nas a2200241   4500008004100000022001400041245015600055210006900211260007300280300001200353490000700365520100800372653002601380653003301406653002201439653001801461653001701479653001001496100002401506700002901530700001701559856012301576       2008                        eng d  a1369-703X00aIntegrated process for extraction and purification of alcohol dehydrogenase from Baker's yeast involving precipitation and aqueous two phase extraction0 aIntegrated process for extraction and purification of alcohol de  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAR  a414-4200 v383 a<p>The potential of aqueous two phase extraction for the purification of alcohol dehydrogenase (ADH) from Baker's yeast (Saccharomyces cerevisiae) is demonstrated. An integrated process involving precipitation followed by aqueous two phase extraction (ATPE) is used for effective recovery of the enzyme. This is carried out in two schemes. In the first scheme, ammonium sulphate precipitation is carried out followed by ATPE of the precipitate after suspending it in buffer. While the second scheme involves polyethylene glycol precipitation followed by ATPE of the supernatant. The effect of various process parameters such as molecular weight of polymer, tie line length, phase volume ratio and neutral salt is investigated for enhancing the activity recovery of ADH. First scheme resulted in about 85% enzyme activity recovery of ADH with purification factor of 4.2 while the second scheme about 90% enzyme activity recovery with 6.6-fold purification. (C) 2007 Elsevier B.V. All rights reserved.</p>10aAlcohol dehydrogenase10aaqueous two phase extraction10aenzyme extraction10aPrecipitation10aPurification10aYeast1 aMadhusudhan, M., C.1 aRaghavarao, K., S. M. S.1 aNene, Sanjay  uhttps://library.ncl.res.in/content/integrated-process-extraction-and-purification-alcohol-dehydrogenase-bakers-yeast-001677nas a2200265   4500008004100000022001400041245009800055210006900153260008600222300001400308490000700322520074600329653001901075653001801094653001801112653001701130653001001147100002101157700002601178700001601204700001801220700002901238700002601267856011801293       2016                        eng d  a0737-393700aEncapsulation of yeast (Saccharomyces cereviciae) by spray drying for extension of shelf life0 aEncapsulation of yeast Saccharomyces cereviciae by spray drying   a530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USAbTAYLOR & FRANCIS INCcJAN  a1307-13180 v343 a<p>The objective of the present work was to encapsulate yeast using different carrier materials and examine their efficacy in retaining viability of cells after spray drying. Slurry containing yeast cells along with known amount of carrier material (maltodextrin, corn starch, gum arabic, acacia gum, polyethylene glycol 8000, -cyclodextrin, and skimmed milk powder, one at a time) was added and served as feed. Among these carrier materials attempted, corn starch and maltodextrin showed the best results with respect to powder yield (59%, w/w) and cell survival (80.5%), respectively. However, considering both survival and powder yield (67 and 59% w/w, respectively), corn starch was observed to be the most suitable carrier material.</p>10aCell viability10aEncapsulation10afreeze-drying10aspray drying10aYeast1 aChandralekha, A.1 aTavanandi, Hrishikesh1 aAmrutha, N.1 aHebbar, Umesh1 aRaghavarao, K., S. M. S.1 aGadre, Ramchandra, V.  uhttps://library.ncl.res.in/content/encapsulation-yeast-saccharomyces-cereviciae-spray-drying-extension-shelf-life01827nas a2200265   4500008004100000022001400041245010900055210006900164260000800233300001200241490000700253520091500260653001801175653001801193653001801211653004701229653002701276653001701303100001901320700002101339700001801360700002201378700002901400856013201429       2018                        eng d  a0737-393700aNonconventional method for drying of Pseudomonas aeruginosa and its comparison with conventional methods0 aNonconventional method for drying of Pseudomonas aeruginosa and   cMAY  a839-8530 v373 a<p>In this study, preparation of dried cultures of Pseudomonas aeruginosa using nonconventional drying method, namely, low-temperature low-humidity (LTLH) drying was investigated. The effect of carrier materials (whey protein, corn starch, and trehalose) was examined one at a time and also in combinations (to explore the synergistic effect). The results were compared with those obtained using spray drying and freeze drying in terms of cell survival and dry cell powder yield. The powder samples were analyzed also for morphology, flowability, particle size, and moisture content. In LTLH drying, good cell survival was observed along with high powder yield when compared with that in spray drying. Corn starch showed the highest cell survival (91%) and powder yield (94%, w/w) among the carrier materials employed besides resulting in good cell survival (65%) even after a storage period of 6 months.</p>
10acell survival10aEncapsulation10afreeze drying10aLow-temperature low-humidity (LTLH) drying10aPseudomonas aeruginosa10aspray drying1 aUmashankar, K.1 aChandralekha, A.1 aDandavate, T.1 aTavanandi, H., A.1 aRaghavarao, K., S. M. S.  uhttps://library.ncl.res.in/content/nonconventional-method-drying-pseudomonas-aeruginosa-and-its-comparison-conventional-methods