01680nas a2200229   4500008004100000022001400041245008200055210006900137260008700206300001400293490000700307520086000314653002001174653001601194653001001210653002001220100003301240700002401273700001901297700001801316856011601334       2006                        eng d  a0011-389100aDirect somatic embryogenesis and plant regeneration in Garcinia indica Choiss0 aDirect somatic embryogenesis and plant regeneration in Garcinia   aC V RAMAN AVENUE, PO BOX 8005, BANGALORE 560 080, INDIAbCURRENT SCIENCE ASSNcOCT  a1074-10780 v913 a<p>Direct somatic embryogenesis without an intervening callus phase was induced from immature seeds of Gar- cinia indica Choiss. Woody plant medium supplemented with 6-benzyl amino purine (BAP) (4.44-22.19 mu m) alone or in combination with a-naphthaleneacetic acid (2.69 mu m) produces somatic embryos within a period of 2-3 weeks with 80% frequency. Embryo induction was observed all over the explant surface. Origin of the embryo was confirmed histologically from sub-epidermal layer of the seed. Maturation of these embryos was achieved after 12 weeks of culture on a medium containing BAP (16.08 mu m) in combination with indole-3-acetic acid (2.85-5.71 mu m) and/or kinetin (4.65 mu m). About 75% of the regenerated somatic embryos germinated into complete plantlets. The plantlets were acclimatized successfully with 92% survival in greenhouse.</p>10aGarcinia indica10aguttifereae10aseeds10asomatic embryos1 aThengane, Shubhada, Ratnakar1 aDeodhar, Swapna, R.1 aBhosle, S., V.1 aRawal, S., K.  uhttps://library.ncl.res.in/content/direct-somatic-embryogenesis-and-plant-regeneration-garcinia-indica-choiss-001830nas a2200241   4500008004100000022001400041245008600055210006900141260010200210300001200312490000700324520094700331653002001278653001601298653003001314653001001344653002001354100003301374700002401407700001901431700001801450856012001468       2006                        eng d  a1054-547600aRepetitive somatic embryogenesis and plant regeneration in Garcinia indica Choiss0 aRepetitive somatic embryogenesis and plant regeneration in Garci  aC/O PUBLISHING DIVISION, NOSWORTHY WAY, WALLINGFORD OX10 8DE, OXON, ENGLANDbCABI PUBLISHINGcMAY  a256-2610 v423 a<p>Immature seeds of Garcinia indica Choiss. were excised from immature fruits and cultured on Lloyd and McCown (1980), woody plant medium (WPM) with different combinations of auxins and cytokinins. Somatic embryos were obtained on the media supplemented with 6-benzylaminopurine (BA; 2.2-22.1 mu M) alone or in combination with alpha-naphthalene acetic acid (NAA; 2.6 mu M) with 80% frequency within a period of 2-3 wk. Subculture of embryos on medium containing BA (16.0 mu M) supplemented with indole-3-acetic acid (IAA; 2.8-5.7 mu M) and/or kinetin (4.6 mu M) gave rise to clusters of secondary somatic embryos along with maturation of primary embryos. In subsequent subculture on hormone-free half-strength WPM, the embryo clusters germinated with an increase in the number of secondary somatic embryos. About 70% of somatic embryos germinated into complete plantlets, which were successfully established under greenhouse conditions.</p>10aGarcinia indica10aguttifereae10asecondary somatic embryos10aseeds10asomatic embryos1 aThengane, Shubhada, Ratnakar1 aDeodhar, Swapna, R.1 aBhosle, S., V.1 aRawal, S., K.  uhttps://library.ncl.res.in/content/repetitive-somatic-embryogenesis-and-plant-regeneration-garcinia-indica-choiss-001611nas a2200229   4500008004100000022001400041245007600055210006900131260008900200300001200289490000700301520081500308653002001123653001601143653001801159653001001177653001101187100002401198700002101222700003301243856010501276       2008                        eng d  a0019-518900aDe novo shoot regeneration from root cultures of garcinia indica choiss0 aDe novo shoot regeneration from root cultures of garcinia indica  aDR K S KRISHNAN MARG, NEW DELHI 110 012, INDIAbNATL INST SCIENCE COMMUNICATIONcJUN  a482-4860 v463 a<p>Roots of plantlets of Garcinia indica when cultured for long time on half strength MS medium supplemented with BAP (0.44-2.22 mu M) showed production of de novo shoots. Roots attached to mother plant showed more number of shoots, while excised root segments produced lesser shoots. Shoots (0.5-0.8 cm) were transferred to elongation medium consisting of Woody Plant Medium (WPM) supplemented with BAP (4.44-22.69 mu M), IAA (5.71 mu M and kinetin (4.65 mu M). It was observed that shoot length increased to 1-2 cm. WPM medium supplemented with NAA (2.69-10.74 mu M) and IBA (4.90 mu M) induced rooting within 20-25 days. Using the present protocol, 20-25 plantlets could be regenerated from single root explant within 3 to 4 months. The protocol has potential for large scale production of elite plants.</p>10aGarcinia indica10aguttifereae10aorganogenesis10aroots10ashoots1 aDeodhar, Swapna, R.1 aThengane, R., J.1 aThengane, Shubhada, Ratnakar  uhttps://library.ncl.res.in/content/de-novo-shoot-regeneration-root-cultures-garcinia-indica-choiss-0