02875nas a2200349   4500008004100000022001400041245015400055210006900209260011000278300001200388490000600400520159700406653002302003653002502026653002902051653002402080100002002104700001702124700001802141700001802159700001802177700001702195700001802212700002402230700001802254700002102272700003002293700002702323700002202350700002402372856012902396       2008                        eng d  a0972-584900aDirect shoot organogenesis and plant regeneration from hypocotyl explants in selected genotypes of Leucaena leucocephala - a leguminous pulpwood tree0 aDirect shoot organogenesis and plant regeneration from hypocotyl  aDR K S KRISHNAN MARG, PUSA CAMPUS, NEW DELHI 110 012, INDIAbNATL INST SCIENCE COMMUNICATION-NISCAIRcJUL  a388-3930 v73 a<p>An efficient in vitro plant regeneration system in subabul (Leucaena leucocephala), a leguminous pulp wood tree species, was established. The induction of shoots was achieved from selected elite clones of subabul K-8, K-636 and also wild type on MS medium supplemented with 2% sucrose and different concentrations (0.88 to 24.6 mu M) of plant growth regulators (BA, Kn, 2iP &amp; TDZ). The best medium for shoot regeneration was MS with 22.2 mu M BA (5 shoots per explant), followed by 22.7 mu M TDZ (4.6 shoots per explant). Addition of putriscine (9.3 mu M) to MS medium containing 22.2 mu M BA enhanced the number of multiple shoots to 7-8 but not the frequency of response. Shoot initials (measuring 1 cm) when separated and transferred on to MS medium containing 1.4 mu M GA(3) elongated to 2-5 cm in 15.20 d with 80% frequency. The per cent frequency of shoot differentiation was almost identical in the genotypes K-8 and K-636 but it differed significantly from the wild type. Leaf yellowing and abscission in all the genotypes was curtailed by supplementing the medium with 685 mu M glutamine or 540 mu M adenine. The excised shoots were transferred to root regeneration media containing 2.46 and 4.98 mu M IBA or 2.6 and 5.3 mu M NAA. Root regeneration was noticed with 100% frequency in all the three genotypes in presence of IBA or NAA. Plantlets were transferred successfully to the pots with 70% survival rate with no visible morphological variations. The protocol can be utilized for mass propagation and genetic transformation studies of this important pulpwood species.</p>10ahypocotyl explants10aLeucaena lucocephala10apulpwood leguminous tree10ashoot organogenesis1 aSirisha, V., L.1 aPrashant, S.1 aRanadheer, D.1 aRamprasad, P.1 aShaik, N., M.1 aArha, Manish1 aGupta, S., K.1 aSrivastava, Sarneer1 aYadav, A., K.1 aKulkarni, P., S.1 aAbhilash, Othalathara, U.1 aKhan, Bashir, Mohammad1 aRawal, Shuban, K.1 aKishor, P., B. Kavi  uhttps://library.ncl.res.in/content/direct-shoot-organogenesis-and-plant-regeneration-hypocotyl-explants-selected-genotypes-002374nas a2200157   4500008004100000245008200041210006900123260002500192300001200217520181200229100001802041700001502059700002002074700001602094856010602110       2016                        eng d00aSalvia miltiorrhiza: A medicinal herb from metabolites to pathway engineering0 aSalvia miltiorrhiza A medicinal herb from metabolites to pathway  aSingaporebSpringer   a215-2383 aThe dried, red-colored roots of Salvia miltiorrhiza (Lamiaceae) also called “danshen” in colloquial language in China have been used in traditional Chinese medicine for thousands of years to treat hypertension and other cardiovascular ailments. The main constituents of danshen are hydrophilic phenolic acids and lipophilic tanshinones. The various uses of danshen in traditional as well as modern medicines have motivated an intensive research on compounds in S. miltiorrhiza. In recent years, more than 110 compounds have been isolated from S. miltiorrhiza and their structure was identified. Tanshinones and their derivatives have been demonstrated to possess properties of slowing down or curing various ailments related to cardiovascular, cerebrovascular, respiratory, liver, nervous system, cancer, Alzheimer’s, and Parkinson’s diseases. With the increasing demand of this herb, an unrestricted collection to supply raw materials and the extraction of its constituents have severely threatened the natural habitats of S miltiorrhiza. This has prompted the researchers to develop alternative strategies for metabolite production. Several in vitro methodologies have been established to generate callus, cell suspension culture, hairy roots, and plant regeneration. Different regulators and elicitors for plant growth have been employed to enhance levels of different constituents. The advent of sequencing technologies, whole genome, and expression data has helped to provide insights and identification of pathway genes involved in the biosynthesis. This book chapter gives a brief description of in vitro methodologies, use of different elicitors, gene functions, genetic modifications, expression profiling for a better understanding, and enhancement of the constituents in S. miltiorrhiza. 1 aGupta, S., K.1 aSharma, P.1 aAgrawal, D., C.1 aTsay, H.-S.  uhttps://library.ncl.res.in/content/salvia-miltiorrhiza-medicinal-herb-metabolites-pathway-engineering01783nas a2200241   4500008004100000022001500041245006600056210006500122260000800187300001400195490000700209520104800216100002501264700001901289700002101308700002001329700001801349700001801367700002401385700001801409700001901427856009501446       2018                        eng d  a 1530-437X00aEvaluation of humidity sensor based on PVP-RGO nanocomposites0 aEvaluation of humidity sensor based on PVPRGO nanocomposites  cNOV  a9097-91040 v183 a<p>his paper describes the humidity sensing properties of one step in situ synthesized poly (N-vinyl pyrrolidone)- reduced graphene oxide (PVP-RGO) nanocomposites. Different PVP-RGO nanocomposites were obtained by varying PVP/GO weight %ratio as x/50 mg, wherein x = 25, 50, and 75 mg, respectively. The real-time adsorption kinetics of the water molecules during the humidity sensing were also discussed using Elovich model, pseudo first and second order equations. The increase in humidity response of 1.5 PVP-RGO nanocomposite is attributed to the presence of large amount of oxygen functionalities. The above observation was corroborated using spectroscopic analysis. The optimized PVP-RGO nanocomposite showed faster response kinetics with response and recovery times of 10 and 20 s, respectively. The present humidity sensor shows remarkable linearity over the range of 20-90%RH. More specifically, sensitivity was correlated with oxygen functionalities created due to variation of PVP during the functionalization of RGO nanosheets.</p>1 aBhangare, Bhagyashri1 aJagtap, Shweta1 aRamgir, Niranjan1 aWaichal, Rupali1 aMuthe, K., P.1 aGupta, S., K.1 aGadkari, Sanjay, C.1 aAswal, D., K.1 aGosavi, Suresh  uhttps://library.ncl.res.in/content/evaluation-humidity-sensor-based-pvp-rgo-nanocomposites